Results 301 to 310 of about 281,013 (338)

Multiplex RT-PCR

2021
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
openaire   +2 more sources

Real-Time Multiplex PCR Assays

Methods, 2001
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra.
C T, Wittwer   +3 more
openaire   +2 more sources

Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic

2012
Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical.
Barletta, Francesca   +2 more
openaire   +3 more sources

Multiplex PCR Design for Scalable Resequencing

2021
While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable solution becoming more routine. Multiplex methods can be applied to virtually any DNA template source (e.g., plant or human DNA, FFPE DNA isolated from clinical samples, bisulfite-converted DNA for DNA ...
Darren, Korbie, Matt, Trau
openaire   +2 more sources

Optimization of Multiplex PCRs

1994
The development of the polymerase chain reaction (PCR) has enabled rapid and efficient analysis of specific DNA sequences (Mullis and Faloona, 1987). Most PCR strategies are designed to amplify one or more target sequences with a single set of oligonucleotide primers.
Jeffrey S. Chamberlain   +1 more
openaire   +1 more source

Home - About - Disclaimer - Privacy