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Development and Validation of In-House Conventional and Multiplex PCR Methods for the Detection and Identification of Lophomonas spp.: An Innovative Approach. [PDF]
Nakhaei M +6 more
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Prevalence and antifungal susceptibility profiles of <i>Candida</i> isolates among patients with candiduria: a multiplex PCR assay. [PDF]
Darabian S +5 more
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Development and clinical validation of a novel multiplex PCR test for detection of respiratory pathogens via fluorescence melting curve analysis. [PDF]
Jiang Y +8 more
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2021
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
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Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
openaire +2 more sources
Real-Time Multiplex PCR Assays
Methods, 2001The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra.
C T, Wittwer +3 more
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Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic
2012Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical.
Barletta, Francesca +2 more
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Multiplex PCR Design for Scalable Resequencing
2021While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable solution becoming more routine. Multiplex methods can be applied to virtually any DNA template source (e.g., plant or human DNA, FFPE DNA isolated from clinical samples, bisulfite-converted DNA for DNA ...
Darren, Korbie, Matt, Trau
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Optimization of Multiplex PCRs
1994The development of the polymerase chain reaction (PCR) has enabled rapid and efficient analysis of specific DNA sequences (Mullis and Faloona, 1987). Most PCR strategies are designed to amplify one or more target sequences with a single set of oligonucleotide primers.
Jeffrey S. Chamberlain +1 more
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