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Optimizing multiplex and LA-PCR with betaine
Trends in Biochemical Sciences, 1997Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and 'long and accurate' polymerase chain reaction (LA-PCR).
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Selecting Degenerate Multiplex PCR Primers
2003Single Nucleotide Polymorphism (SNP) Genotyping is an important molecular genetics technique in the early stages of producing results that will be useful in the medical field. One of the proposed methods for performing SNP Genotyping requires amplifying regions of DNA surrounding a large number of SNP loci. In order to automate a portion of this method
Richard Souvenir +3 more
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Médecine Intensive Réanimation
La polymerase chain reaction (PCR) est une technique de biologie moléculaire d’importance fondamentale qui permet de détecter et quantifier des fragments spécifiques d’acides nucléiques. En combinant plusieurs primers dans une même réaction, il est possible de détecter de manière simultanée plusieurs cibles moléculaires distinctes, ce qui défi nit la PCR ...
Louis Kreitmann +2 more
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La polymerase chain reaction (PCR) est une technique de biologie moléculaire d’importance fondamentale qui permet de détecter et quantifier des fragments spécifiques d’acides nucléiques. En combinant plusieurs primers dans une même réaction, il est possible de détecter de manière simultanée plusieurs cibles moléculaires distinctes, ce qui défi nit la PCR ...
Louis Kreitmann +2 more
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Anchored multiplex PCR for targeted next-generation sequencing
Nature Medicine, 2014Zongli Zheng +13 more
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Diagnostic microbiology and infectious disease, 2018
C. Morgenstern +4 more
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C. Morgenstern +4 more
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[Multiplex PCR in clinical microbiology].
Enfermedades infecciosas y microbiologia clinica, 2004The introduction of molecular biology methods in clinical microbiology laboratories brings important insights to obtain sensitive and specific diagnoses as fast as possible. These methods are not intended for replacement but for complement of the already applied microbiologic methods.
Sebastián, Méndez-Alvarez +1 more
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