Results 301 to 310 of about 1,719,427 (359)

A multiplex PCR assay combined with capillary electrophoresis for the simultaneous detection of tropomyosin allergens from oyster, mussel, abalone, and clam mollusk species.

Food Chemistry, 2020
Tropomyosin present in mollusk species is the most common allergen in humans and causes excessive immune responses. To simultaneously detect tropomyosin allergens in mollusk species, a multiplex PCR assay combined with capillary electrophoresis was ...
Seung-Man Suh, Mi-Ju Kim, Hee-In Kim, Hyun-Joong Kim, Hae-Yeong Kim   +4 more
semanticscholar   +1 more source

Fast multiplex bacterial PCR of bronchoalveolar lavage for antibiotic stewardship in hospitalised patients with pneumonia at risk of Gram-negative bacterial infection (Flagship II): a multicentre, randomised controlled trial.

The Lancet Respiratory Medicine, 2022
BACKGROUND PCR-based testing has transformed the management of suspected respiratory viral infections. We aimed to determine whether multiplex bacterial PCR of bronchoalveolar lavage fluid aids antibiotic stewardship in patients with pneumonia ...
A. Darie, N. Khanna, K. Jahn, Michael Osthoff, S. Bassetti, Mirjam Osthoff, D. Schumann, W. Albrich, H. Hirsch, M. Brutsche, L. Grize, M. Tamm, D. Stolz   +12 more
semanticscholar   +1 more source

Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic

2012
Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical.
Barletta, Francesca, Ochoa Woodell, Theresa Jean, Cleary, Thomas G.   +2 more
openaire   +3 more sources

Multiplex PCR for detection of Enterobacteriaceae in blood

Transfusion, 2001
BACKGROUND: Rapid and sensitive methods are needed to detect the small numbers of bacteria that may sometimes contaminate units of blood during collection. A multiplex 5′‐nuclease TaqMan PCR assay (PE Applied Biosystems) was used to detect several bacterial species that may contaminate blood.STUDY DESIGN AND METHODS: Oligonucleotide primers were made ...
K, Sen, D M, Asher
openaire   +2 more sources

Real-Time Multiplex PCR Assays

Methods, 2001
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra.
C T, Wittwer, M G, Herrmann, C N, Gundry, K S, Elenitoba-Johnson   +3 more
openaire   +2 more sources

Development of a touch-down multiplex PCR method for simultaneously rapidly detecting three novel insertion/deletions (indels) within one gene: an example for goat GHR gene

Animal Biotechnology, 2018
The multiplex polymerase chain reaction (PCR) method is fast and accuracy for screening several polymorphism loci in a single reaction in large samples.
Qing Yang, Shaoli Zhang, Jie Li, Xinyu Wang, Kun Peng, X. Lan, C. Pan   +6 more
semanticscholar   +1 more source

Optimization of Multiplex PCRs

1994
The development of the polymerase chain reaction (PCR) has enabled rapid and efficient analysis of specific DNA sequences (Mullis and Faloona, 1987). Most PCR strategies are designed to amplify one or more target sequences with a single set of oligonucleotide primers.
Jeffrey S. Chamberlain, Joel R. Chamberlain   +1 more
openaire   +1 more source

Multiplex-Tandem PCR for Fungal Diagnostics

2012
Multiplex, real-time PCR has become an invaluable tool for the rapid identification of pathogens in clinical specimens enabling earlier and more targeted management of antimicrobial therapy. In this chapter, we describe the methodology behind a novel multiplex-tandem PCR (MT-PCR) platform designed for the rapid identification of up to 18 fungal ...
Anna, Lau, Keith, Stanley, Tania, Sorrell   +2 more
openaire   +2 more sources

Hydrogels for Efficient Multiplex PCR

Biotechnology and Bioprocess Engineering, 2020
Multiplex PCR simultaneously detects several different DNA targets in a given sample and has, therefore, gained considerable interest for use in examining a genetic target whose number rapidly increases in a single phenotype. Conventional real-time PCR lacks sufficient multiplex capacity, as the limited number of color channels restricts the number of ...
Juny Shin, Cheulhee Jung
openaire   +1 more source

Multiplex PCR Amplification of Ancient DNA

2011
Multiplex PCR allows the simultaneous amplification of up to dozens of target fragments in a single PCR. It is therefore a powerful tool to obtain many kilobases of continuous sequence from minute amounts of ancient DNA (aDNA), which usually must be amplified in multiple short and overlapping fragments.
Mathias, Stiller, Tara L, Fulton
openaire   +2 more sources