Results 311 to 320 of about 279,791 (361)

Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic

2012
Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical.
Barletta, Francesca, Ochoa Woodell, Theresa Jean, Cleary, Thomas G.   +2 more
openaire   +3 more sources

Multiplex PCR Design for Scalable Resequencing

2021
While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable solution becoming more routine. Multiplex methods can be applied to virtually any DNA template source (e.g., plant or human DNA, FFPE DNA isolated from clinical samples, bisulfite-converted DNA for DNA ...
Darren, Korbie, Matt, Trau
openaire   +2 more sources

Optimization of Multiplex PCRs

1994
The development of the polymerase chain reaction (PCR) has enabled rapid and efficient analysis of specific DNA sequences (Mullis and Faloona, 1987). Most PCR strategies are designed to amplify one or more target sequences with a single set of oligonucleotide primers.
Jeffrey S. Chamberlain, Joel R. Chamberlain   +1 more
openaire   +1 more source

Hydrogels for Efficient Multiplex PCR

Biotechnology and Bioprocess Engineering, 2020
Multiplex PCR simultaneously detects several different DNA targets in a given sample and has, therefore, gained considerable interest for use in examining a genetic target whose number rapidly increases in a single phenotype. Conventional real-time PCR lacks sufficient multiplex capacity, as the limited number of color channels restricts the number of ...
Juny Shin, Cheulhee Jung
openaire   +1 more source

PCR multiplex en réanimation

Médecine Intensive Réanimation
La polymerase chain reaction (PCR) est une technique de biologie moléculaire d’importance fondamentale qui permet de détecter et quantifier des fragments spécifiques d’acides nucléiques. En combinant plusieurs primers dans une même réaction, il est possible de détecter de manière simultanée plusieurs cibles moléculaires distinctes, ce qui défi nit la PCR ...
Louis Kreitmann, Alexandre Gaudet, Guillaume Voiriot   +2 more
openaire   +1 more source

Multiplex-Tandem PCR for Fungal Diagnostics

2012
Multiplex, real-time PCR has become an invaluable tool for the rapid identification of pathogens in clinical specimens enabling earlier and more targeted management of antimicrobial therapy. In this chapter, we describe the methodology behind a novel multiplex-tandem PCR (MT-PCR) platform designed for the rapid identification of up to 18 fungal ...
Anna, Lau, Keith, Stanley, Tania, Sorrell   +2 more
openaire   +2 more sources

Multiplex PCR Amplification of Ancient DNA

2011
Multiplex PCR allows the simultaneous amplification of up to dozens of target fragments in a single PCR. It is therefore a powerful tool to obtain many kilobases of continuous sequence from minute amounts of ancient DNA (aDNA), which usually must be amplified in multiple short and overlapping fragments.
Mathias, Stiller, Tara L, Fulton
openaire   +2 more sources

Multiplex nested PCR for Brazilian Alphavirus diagnosis

Transactions of the Royal Society of Tropical Medicine and Hygiene, 2004
A reverse-transcriptase PCR (RT-PCR) and a multiplex nested PCR were developed for the rapid detection and identification of 14 Brazilian alphaviruses. Using Alphavirus genus-specific primers in a RT-PCR, we obtained amplified products of 434 bp. Species-specific primers were selected and simultaneously tested in a multiplex nested PCR.
Roberta V M, Bronzoni, Marcos L, Moreli, Ana Cecília R, Cruz, Luiz Tadeu M, Figueiredo   +3 more
openaire   +2 more sources

Selecting Degenerate Multiplex PCR Primers

2003
Single Nucleotide Polymorphism (SNP) Genotyping is an important molecular genetics technique in the early stages of producing results that will be useful in the medical field. One of the proposed methods for performing SNP Genotyping requires amplifying regions of DNA surrounding a large number of SNP loci. In order to automate a portion of this method
Richard Souvenir, Jeremy Buhler, Gary Stormo, Weixiong Zhang   +3 more
openaire   +1 more source

Monochrome Multiplex Quantitative PCR Telomere Length Measurement

Journal of Visualized Experiments
Telomeres are ribonucleoprotein structures at the end of all eukaryotic chromosomes that protect DNA from damage and preserve chromosome stability. Telomere length (TL) has been associated with various exposures, biological processes, and health outcomes.
Noelle A, Martin, Lauren W Y, McLester-Davis, Trevor R, Roy, Madeline G, Magruder, Waylon J, Hastings, Stacy S, Drury   +5 more
openaire   +2 more sources