Results 11 to 20 of about 18,173,972 (253)

Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples [PDF]

BMC Microbiology, 2008
Background A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, Kentucky and Dublin.
Whyte Paul, Duffy Geraldine, Barry Thomas, McGuinness Sheila, Burgess Catherine, McCabe Evonne, O'Regan Edel, Fanning Séamus   +7 more
doaj   +2 more sources

Diagnosis of Rickettsial infection by Multiplex Real-time PCR in Febrile Pediatric Patients Admitted in a Tertiary Care Hospital at Chennai, South India [PDF]

Journal of Pure and Applied Microbiology
Rickettsioses caused by Rickettsia and Orientia spp. are the re-emerging diseases in India, which are grossly underdiagnosed, particularly among children.
Rajagopal Murali, Sivasambo Kalpana, Karthick Shanmugam, Prabu Dhandapani   +3 more
doaj   +2 more sources

Evaluating an MRNA based body fluid identification test using sybr green fluorescent dye and real-time PCR [PDF]

, 2011
The requirement to have more definitive and wider ranging body fluid identification (BFI) tests has resulted in a range of mRNA based real-time PCR BFI assays utilising Taqman fluorescent dye.
Connolly, Jo-Ann, Williams, Graham
core   +3 more sources

Simplified development of multiplex real-time PCR through master mix augmented by universal fluorogenic reporters

BioTechniques, 2016
Mediator probe (MP) PCR is a real-time PCR approach that uses standardized universal fluorogenic reporter oligonucleotides (UR) in conjunction with label-free sequence-specific probes.
Simon Wadle, Michael Lehnert, Friedrich Schuler, René Köppel, Annerose Serr, Roland Zengerle, Felix von Stetten   +6 more
doaj   +1 more source

Rapid detection and molecular survey of blaVIM, blaIMP and blaNDM genes among clinical isolates of Acinetobacter baumannii using new multiplex real-time PCR and melting curve analysis

BMC Microbiology, 2019
Background Acinetobacter baumannii is a cosmopolitan bacterium that is frequently reported from hospitalized patients, especially those patients who admitted in the intensive care unit.
Hossein Goudarzi, Elnaz Sadat Mirsamadi, Zohreh Ghalavand, Mojdeh Hakemi Vala, Hamed Mirjalali, Ali Hashemi   +5 more
doaj   +1 more source

Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis

Canadian Journal of Infectious Diseases and Medical Microbiology, 2020
Background and Objective. Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Pseudomonas aeruginosa, and Mycobacterium tuberculosis are primary respiratory bacterial ...
Liuyang Hu, Bing Han, Qin Tong, Hui xiao, Donglin Cao   +4 more
doaj   +1 more source

Molecular detection of human Plasmodium species using a multiplex real time PCR

Scientific Reports, 2023
Molecular detection methods have revealed higher sensitivity and specificity than conventional microscopy or rapid diagnostic tests for malaria diagnosis.
Yassamine Lazrek, Célia Florimond, Béatrice Volney, Manon Discours, Emilie Mosnier, Sandrine Houzé, Stéphane Pelleau, Lise Musset   +7 more
doaj   +1 more source

Rapid and Sensitive Qualitative Duoplex Real-Time PCR Method for Discriminatory and Confirmatory Diagnosis of HTLV-1 and HTLV-2 Infections: Brazilian Multicentric Study

Frontiers in Medicine, 2022
Human T cell lymphotropic virus (HTLV) is the caustive agent of two main conditions i. e., the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and the adult T-cell leukemia/lymphoma (ATLL).
Mauricio Cristiano Rocha-Junior, Mauricio Cristiano Rocha-Junior, Evandra Strazza Rodrigues, Evandra Strazza Rodrigues, Svetoslav Nanev Slavov, Svetoslav Nanev Slavov, Tatiane Assone, Maíra Pedreschi, Debora Glenda Lima de La Roque, Debora Glenda Lima de La Roque, Maisa Sousa, Viviana Olavarria, Bernardo Galvão-Castro, Benedito Antonio Lopes da Fonseca, Augusto César Penalva de Oliveira, Jerusa Smid, Oswaldo Massaiti Takayanagui, Jorge Casseb, Dimas Tadeu Covas, Dimas Tadeu Covas, Dimas Tadeu Covas, Simone Kashima, Simone Kashima, Simone Kashima   +23 more
doaj   +1 more source

EVALUATION OF MULTIPLEX REAL-TIME PCR IN DETECTION OF ENTERIC PATHOGENIC PROTOZOA AMONG DIARRHEIC CHILDREN [PDF]

Journal of the Egyptian Society of Parasitology, 2022
Gastrointestinal protozoa are one of the global health problems, particularly in the Eastern MediterraneanCountries. Urgent parasitological diagnosis is indicated to start proper treatment.The present study evaluated multiplex real-time (RT) PCR and ...
HEBA IBRAHIM, OMAR HAMEED, TALAL SAEED, MOHAMMAD ABDELRAHMAN, MONA EL-SAYAD, NAGLAA ABD EL-LATIF   +5 more
doaj   +1 more source

Successful gene expression analysis by multiplex, real-time, one-step RT-PCR, irrespective of the targets amplified

BioTechniques, 2007
Multiplex, real-time, one-step RT-PCR enables simultaneous quantification of several transcripts in one reaction vessel. This saves time and conserves precious sample material when analyzing the expression of reference and target genes in a particular ...
Holger Engel, Corinna Kueppers, Margaretha Koenig, Dirk Loeffert   +3 more
doaj   +1 more source