Fine-Tuning Multiprotein Complexes Using Small Molecules [PDF]
ACS Chemical Biology, 2012 Multiprotein complexes such as the transcriptional machinery, signaling hubs, and protein folding machines are typically composed of at least one enzyme combined with multiple non-enzymes. Often the components of these complexes are incorporated in a combinatorial manner, in which the ultimate composition of the system helps dictate the type, location,
Thompson, Andrea D +3 more
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Baculovirus expression: tackling the complexity challenge [PDF]
, 2013 This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
Barford, David +3 more
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Structure-function mapping of a heptameric module in the nuclear pore complex. [PDF]
, 2012 The nuclear pore complex (NPC) is a multiprotein assembly that serves as the sole mediator of nucleocytoplasmic exchange in eukaryotic cells. In this paper, we use an integrative approach to determine the structure of an essential component of the yeast ...
Chait, Brian T +10 more
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Purifi cation of Multiprotein Histone Acetyltransferase Complexes [PDF]
, 2011 The reversible acetylation of specific lysine residues on core histones regulates gene transcription in eukaryotes. Since the discovery of GCN5 as the first transcription-regulating histone acetyltransferase (HAT), a variety of HATs have now been identified and shown to acetylate different sites on histones as well as on non-histone proteins, including
Yuan-Liang, Wang +2 more
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Distinct and additive effects of calorie restriction and rapamycin in aging skeletal muscle
Nature Communications, 2022 The anti-aging intervention calorie restriction (CR) is thought to act via the nutrient-sensing multiprotein complex mTORC1. Here the authors show that the mTORC1-inhibitor rapamycin and CR use largely distinct mechanisms to slow mouse muscle aging.
Daniel J. Ham +15 more
doaj +1 more source
Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato [PDF]
, 2008 Transcriptional co-activators of the Multiprotein Bridging Factor1 (MBF1) type belong to a multigenic family that encode key components of the machinery controlling gene expression by communicating between transcription factors and the basal ...
Bouzayen, Mondher +8 more
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MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells
Viruses, 2019 The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryotic proteins for many applications, ranging from structure analysis to drug screening and the provision of protein biologics and therapeutics.
Kapil Gupta +5 more
doaj +1 more source
The eEF1 family of mammalian translation elongation factors
BBA Advances, 2023 The eEF1 family of mammalian translation elongation factors is comprised of the two variants of eEF1A (eEF1A1 and eEF1A2), and the eEF1B complex. The latter consists of eEF1Bα, eEF1Bβ, and eEF1Bγ subunits.
B.S. Negrutskii +5 more
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The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics [PDF]
, 2016 Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology.
Berger, Imre, Gupta, Kapil
core +2 more sources
Xyloglucan and its biosynthesis
Frontiers in Plant Science, 2012 The hemicellulosic polysaccharide xyloglucan (XyG), found in the primary cell walls of most plant tissues, is important for structural organization of the cell wall and regulation of growth and development.
Olga A Zabotina
doaj +1 more source