A Mammalian Mediator Subunit that Shares Properties with Saccharomyces cerevisiae Mediator Subunit Cse2 [PDF]
, 2004 The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. We previously identified and partially purified a mammalian Mediator complex from rat liver nuclei (Brower ...
Banks, Charles A. S. +10 more
core +1 more source
Structure of a bacterial type IV secretion core complex at subnanometre resolution [PDF]
, 2013 Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4.
Chandran, Vidya +10 more
core +1 more source
Multiprotein complex production in insect cells by using polyproteins. [PDF]
Methods Mol Biol, 2014 A powerful approach utilizing polyproteins for balancing stoichiometry of recombinant multiprotein complexes overproduced in baculovirus expression vector systems (BEVS) is described. This procedure has been implemented here in the MultiBac system but can also be directly adapted to all commonly used BEVS.
Nie Y +4 more
europepmc +4 more sources
Lighting up multiprotein complexes: lessons from GPCR oligomerization [PDF]
Trends in Biotechnology, 2010 Spatiotemporal characterization of protein-protein interactions (PPIs) is essential in determining the molecular mechanisms of intracellular signaling processes. In this review, we discuss how new methodological strategies derived from non-invasive fluorescence- and luminescence-based approaches (FRET, BRET, BiFC and BiLC), when applied to the study of
Ciruela Alférez, Francisco +2 more
openaire +3 more sources
Identification of Mammalian Mediator Subunits with Similarities to Yeast Mediator Subunits Srb5, Srb6, Med11, and Rox3 [PDF]
, 2003 The Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transactivators. We recently identified a mammalian homologue of yeast Mediator subunit Med8 and partially purified a Med8-containing ...
Banks, Charles A. S. +11 more
core +2 more sources
Inflammasomes driven inflammation in lung cancer revisited: a short review
Exploration of Immunology, 2023 Lung cancer is the leading cause of cancer-related deaths worldwide. The main risk factor for lung cancer is exposure to chemicals present in cigarettes and atmospheric pollutants, which, among other mechanisms, can increase the risk of cancer by ...
Vitor Rodrigues da Costa +5 more
doaj +1 more source
DNA recognition by the herpes simplex virus transactivator VP16: a novel DNA-Binding structure [PDF]
, 2001 Upon infection, the herpes simplex virus (HSV) transcriptional activator VP16 directs the formation of a multiprotein-DNA complex-the VP16-induced complex-with two cellular proteins, the host cell factor HCF-1 and the POU domain transcription factor Oct ...
Babb, R. +3 more
core +1 more source
Towards molecular systems biology of gene transcription and regulation [PDF]
, 2010 Ten years after the determination of the RNA polymerase 11 structure, the basic mechanism of mRNA synthesis during gene transcription is known. In the future, the initiation and regulation of transcription must be studied with a combination of structural
Cramer, Patrick
core +1 more source
A Mammalian Homolog of Drosophila melanogaster Transcriptional Coactivator Intersex Is a Subunit of the Mammalian Mediator Complex [PDF]
, 2003 The multiprotein Mediator complex is a coactivator required for transcriptional activation of RNA polymerase II transcribed genes by DNA binding transcription factors.
Banks, Charles A. S. +7 more
core +1 more source
The Stoichiometric Divisome: A Hypothesis
Frontiers in Microbiology, 2015 Dividing Escherichia coli cells simultaneously constrict the inner membrane, peptidoglycan layer and outer membrane to synthesize the new poles of the daughter cells. For this, more than 30 proteins localize to mid-cell where they form a large, ring-like
Waldemar eVollmer +1 more
doaj +1 more source