Results 51 to 60 of about 73,884 (236)
Visualization of Multiprotein Complexes by Flow Cytometry [PDF]
Current Protocols in Immunology, 2009 AbstractMultiprotein complexes and other protein‐protein interactions play important roles in virtually all cellular processes. Analysis of coimmunoprecipitation of protein complexes by flow cytometry (IP‐FCM, or “the fly‐p” method) provides a sensitive means to measure these interactions in the native/nondenatured state.
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Polyomavirus T Antigens: Molecular Chaperones for Multiprotein Complexes [PDF]
Journal of Virology, 1998 Simian virus 40 (SV40) and murine polyomavirus (PyV) serve as powerful tools to identify key cellular regulatory proteins and to discern the mechanisms by which these proteins exert their biological effects. Like all members of the polyomavirus group, SV40 and PyV have simple genomes (Fig.
J L, Brodsky, J M, Pipas
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Partial Purification of a Megadalton DNA Replication Complex by Free Flow Electrophoresis.
PLoS ONE, 2016 We describe a gentle and rapid method to purify the intact multiprotein DNA replication complex using free flow electrophoresis (FFE). In particular, we applied FFE to purify the human cell DNA synthesome, which is a multiprotein complex that is fully ...
Caroline M Li +4 more
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Selective endocytosis controls slit diaphragm maintenance and dynamics in Drosophila nephrocytes
eLife, 2022 The kidneys generate about 180 l of primary urine per day by filtration of plasma. An essential part of the filtration barrier is the slit diaphragm, a multiprotein complex containing nephrin as major component.
Konrad Lang +12 more
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Hsp90-binding immunophilins link p53 to dynein during p53 transport to the nucleus [PDF]
, 2004 The tumor suppressor protein p53 is known to be transported to the nucleus along microtubular tracks by cytoplasmic dynein. However, the connection between p53 and the dynein motor protein complex has not been established.
Galigniana, Mario Daniel +4 more
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Multiprotein complex formation on the c‐myc promoter [PDF]
IUBMB Life, 1997 AbstractThe E2F element is a cis‐acting DNA sequence within the P2 promoter of the c‐myc proto‐oncogene. While it is required for optimal transcription, the multiprotein complexes formed on this site have not been well characterized. We show that in extracts of human glioblastoma cells and NIH3T3 fibroblasts, significant E2F transcription factor ...
T J, Logan, K H, Moberg, D J, Hall
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Cell Death Discovery, 2022 Colorectal cancer is a major contributor to the worldwide prevalence of cancer-related deaths. Metastasis and chemoresistance are the two main causes for colorectal cancer treatment failure, and thus, high mortality.
Ruijun Pan +8 more
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Multiprotein Expression Strategy for Structural Biology of Eukaryotic Complexes [PDF]
Structure, 2007 The concept of the cell as a collection of multisubunit protein machines is emerging as a cornerstone of modern biology, and molecular-level study of these machines in most cases will require recombinant production. Here, we present and validate a strategy to rapidly produce, permutate, and posttranslationally modify large, eukaryotic multiprotein ...
Fitzgerald, Daniel J +6 more
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Editosome Accessory Factors KREPB9 and KREPB10 in Trypanosoma brucei [PDF]
, 2012 Multiprotein complexes, called editosomes, catalyze the uridine insertion and deletion RNA editing that forms translatable mitochondrial mRNAs in kinetoplastid parasites.
Acestor +58 more
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Ion channels as part of macromolecular multiprotein complexes [PDF]
Herzschrittmachertherapie + Elektrophysiologie, 2017 Ion channels and Ca2+-handling proteins involved in the regulation of cardiac electrophysiology and contractility are organized in macromolecular multiprotein complexes. Recent molecular and cellular studies have significantly enhanced our understanding of the composition of these macromolecular complexes and have helped to elucidate their role in the ...
Heijman, Jordi, Dobrev, Dobromir
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