Results 101 to 110 of about 40,419 (211)

The production of multiprotein complexes in insect cells using the baculovirus expression system

, 2014
The production of a homogeneous protein sample in sufficient quantities is an essential prerequisite not only for structural investigations but represents also a rate-limiting step for many functional studies.
Abdulrahman, Wassim, Olga Kolesnikova, Garzoni, Frederic, Gupta, Kapil, Arnaud Poterszman, Wassim Abdulrahman, Laura Radu, Frederic Garzoni, Berger, Imre, Poterszman, Arnaud, Kolesnikova Karchenkov, Olga, Judit Osz-Papai, Imre Berger, Kapil Gupta, Osz-Papai, Judit, Kolesnikova, Olga, Radu, Laura   +16 more
core   +1 more source

When proteases reshape barriers: Basement membrane remodelling in development, wound healing and tumour progression

The FEBS Journal, EarlyView.
Basement membrane (BM) homeostasis relies on a balance between integrity, controlled remodelling and pathological degradation. Increased protease expression beneath the BM, along with reduced levels of endogenous protease inhibitors, drives the transition from a continuous, protective barrier to a discontinuous interface.
Clara Legendre, Guila Dayan, Patricia Rousselle   +2 more
wiley   +1 more source

Modification of the composition of polycystin-1 multiprotein complexes by calcium and tyrosine phosphorylation

, 2000
Mutations in the PKD1 gene are responsible for >85% of autosomal dominant polycystic kidney disease (ADPKD). The protein product of PKD1, polycystin-1, is a large, modular membrane protein, with putative ligand-binding motifs in the extracelluar N ...
Wilson, Patricia D., Christopher R. Burrow, Burrow, Christopher R., Li, Hsi-Ping, Lin Geng, Geng, Lin, Patricia D. Wilson, Hsi-Ping Li   +7 more
core   +1 more source

Systemwide disassembly and assembly of SCF ubiquitin ligase complexes

, 2023
Cells respond to environmental cues by remodeling their inventories of multiprotein complexes. Cellular repertoires of SCF (SKP1-CUL1-F box protein) ubiquitin ligase complexes, which mediate much protein degradation, require CAND1 to distribute the ...
Henneberg, L., Daniel C. Scott, Lukas T. Henneberg, Matthias Mann, Baek, K., King, M., Mann, M., Kheewoong Baek, Moeko T. King, Scott, D., Brenda A. Schulman, Schulman, B.   +11 more
core   +1 more source

Epigenetic Regulation in the Pathogenesis of Periodontitis

Journal of Periodontal Research, EarlyView.
The aim of this narrative literature review was to identify epigenetic marks associated with periodontitis and to place them in a biological context. The literature was reviewed based on pre‐defined criteria. Cell type specific chromatin and mRNA modifications were included.
Henrik Dommisch, Arne Sönke Schäfer, Denica Kuzmanova, Dominique S. Michaud   +3 more
wiley   +1 more source

Equi‐MOI ratio for rapid baculovirus‐mediated multiprotein co‐expression in insect cells integrating selenomethionine for structural studies

FEBS Open Bio
Proteins often co‐exist as multicomponent assemblies, making their co‐expression essential in recombinant production processes. The baculovirus expression vector system is commonly used to produce recombinant multiprotein complexes mostly for structural ...
Andrej Bitala, Mário Benko, Marek Nemčovič, Ivana Nemčovičová   +3 more
doaj   +1 more source

Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes

Data in Brief, 2016
Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis ...
Sadhna Phanse, Cuihong Wan, Blake Borgeson, Fan Tu, Kevin Drew, Greg Clark, Xuejian Xiong, Olga Kagan, Julian Kwan, Alexandr Bezginov, Kyle Chessman, Swati Pal, Graham Cromar, Ophelia Papoulas, Zuyao Ni, Daniel R. Boutz, Snejana Stoilova, Pierre C. Havugimana, Xinghua Guo, Ramy H. Malty, Mihail Sarov, Jack Greenblatt, Mohan Babu, W. Brent Derry, Elisabeth R. Tillier, John B. Wallingford, John Parkinson, Edward M. Marcotte, Andrew Emili   +28 more
doaj   +1 more source

The role of amyloid precursor proteins : studies using neurons generated from wild-type and mutant embryonic stem cells [PDF]

, 2006
Alzheimer′s Disease (AD) is the most common neurodegenerative disorder in elderly people. It is becoming an ever larger problem due to prolonged life-expectancies in developed countries.
Schrenk, Katrin
core   +1 more source

CNGCs in Marchantia paleacea uncouple arbuscular mycorrhizal symbiosis and rhizoid development

New Phytologist, EarlyView.
Rhizoid growth and AM fungal infection are uncoupled. Summary In Marchantia paleacea, MpaDMI1‐dependent nuclear Ca2+ oscillations are essential for arbuscular mycorrhizal (AM) fungal colonisation, indicating that endosymbiosis‐mediated nuclear Ca2+ signalling is a conserved feature of land plant–AM symbiosis.
Anson Ho Ching Lam, Aisling Cooke, Jake Richardson, Myriam Charpentier   +3 more
wiley   +1 more source

In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2–7 hexamer dimerization [PDF]

, 2013
26.03.14 KB.
Brill, S, Lurz, R, Herrera, MC, Fernández-Cid, A, Speck, C, Clarke, P, Evrin, C, Zech, J, Riera, A   +8 more
core   +1 more source