Results 21 to 30 of about 6,296 (200)

Breaking barriers: pCF10 type 4 secretion system relies on a self-regulating muramidase to modulate the cell wall. [PDF]

mBio
Conjugative type 4 secretion systems (T4SSs) are the main driver for the spread of antibiotic resistance genes and virulence factors in bacteria.
Sun W-S, Torrens G, Ter Beek J, Cava F, Berntsson RP-A.   +4 more
europepmc   +2 more sources

Structural insights into thrombolytic activity of destabilase from medicinal leech

Scientific Reports, 2023
Destabilase from the medical leech Hirudo medicinalis belongs to the family of i-type lysozymes. It has two different enzymatic activities: microbial cell walls destruction (muramidase activity), and dissolution of the stabilized fibrin (isopeptidase ...
Egor Marin, Daniil A. Kornilov, Sergey S. Bukhdruker, Vladimir A. Aleksenko, Valentin A. Manuvera, Egor V. Zinovev, Kirill V. Kovalev, Mikhail B. Shevtsov, Anna A. Talyzina, Pavel A. Bobrovsky, Pavel K. Kuzmichev, Alexey V. Mishin, Ivan Y. Gushchin, Vassili N. Lazarev, Valentin I. Borshchevskiy   +14 more
doaj   +1 more source

Biopsy-proven sarcoidosis lesion of the levator palpebrae superioris causing myogenic blepharoptosis. [PDF]

Am J Ophthalmol Case Rep
Purpose: We describe a case of myogenic blepharoptosis due to sarcoidosis directly involving the levator palpebrae superioris, confirmed with biopsy.
Firn KA, Hawy E, Van Putten DJ.
europepmc   +2 more sources

A probiotic bi-functional peptidoglycan hydrolase sheds NOD2 ligands to regulate gut homeostasis in female mice

Nature Communications, 2023
Secreted proteins are one of the direct molecular mechanisms by which microbiota influence the host, thus constituting a promising field for drug discovery.
Jie Gao, Lei Wang, Jing Jiang, Qian Xu, Nianyi Zeng, Bingyun Lu, Peibo Yuan, Kai Sun, Hongwei Zhou, Xiaolong He   +9 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Safety and efficacy of muramidase from Trichoderma reesei DSM 32338 as a feed additive for turkeys for fattening, turkeys reared for breeding, chickens reared for breeding and other poultry species reared for breeding

EFSA Journal, 2019
Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of muramidase produced by Trichoderma reesei DSM 32338 ...
EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), Vasileios Bampidis, Giovanna Azimonti, Maria de Lourdes Bastos, Henrik Christensen, Birgit Dusemund, Maryline Kouba, Mojca Kos Durjava, Marta López‐Alonso, Secundino López Puente, Francesca Marcon, Baltasar Mayo, Alena Pechová, Mariana Petkova, Fernando Ramos, Yolanda Sanz, Roberto Edoardo Villa, Ruud Woutersen, Montserrat Anguita, Jaume Galobart, Paola Manini, Fabiola Pizzo, Jordi Tarrés‐Call, Orsolya Holczknecht   +23 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

VAGINAL LACTOBACILLI REGULATE THE ACTIVITY OF MURAMIDASE VIA HYDROGEN PEROXIDE AND SURFACTANTS

Журнал микробиологии, эпидемиологии и иммунобиологии, 2018
Aim. Evaluation of the effect of metabolites of vaginal lactobacilli on the enzymatic and bactericidal activity of muramidase. Materials and methods. We investigated how the enzymatic and bactericidal activity of muramidase changes after treatment with ...
A. V. Sgibnev, E. A. Kremleva
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source