Revisiting the Endocytosis of the M2 Muscarinic Acetylcholine Receptor [PDF]
Membranes, 2015 The agonist-induced endocytosis of the muscarinic acetylcholine receptor M2 is different from that of the other members of the muscarinic receptor family.
Wymke Ockenga, Ritva Tikkanen
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Acetylcholine muscarinic M2 receptor maintains human Schwann-like adipose-derived phenotype in the absence of differentiating medium. [PDF]
Cell Death DiscovHuman adipose-derived stem cells (ASCs), differentiated in vitro towards Schwann-like phenotype (hdASCs), have been suggested as an alternative source of Schwann cells (SCs).
Piovesana R, Faroni A, Tata AM, Reid AJ.
europepmc +3 more sources
Trafficking of M2 Muscarinic Acetylcholine Receptors [PDF]
Journal of Biological Chemistry, 1999 Internalization is an important mechanism regulating the agonist-dependent responses of G-protein-coupled receptors. The internalization of the M(2) muscarinic cholinergic receptors (mAChR) in HEK293 cells has been demonstrated to occur by an unknown mechanism that is independent of arrestins and dynamin.
Aaron G. Roseberry, M. Marlene Hosey
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Expression of Functional M2 Muscarinic Acetylcholine Receptor in Escherichia coli [PDF]
Journal of Biochemistry, 2000 The M2 muscarinic acetylcholine receptor mutant (M2 mutant), with a lack of glycosylation sites, a deletion in the central part of the third inner loop, and the addition of a six histidine tag at the C-terminus, was fused to maltose binding protein (MBP) at its N-terminus and expressed in Escherichia coli. The expression level was 0.2 nmol receptor per
Hiroyasu Furukawa, Tatsuya Haga
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Notch Signal Mediates the Cross-Interaction between M2 Muscarinic Acetylcholine Receptor and Neuregulin/ErbB Pathway: Effects on Schwann Cell Proliferation [PDF]
Biomolecules, 2022 The cross-talk between axon and glial cells during development and in adulthood is mediated by several molecules. Among them are neurotransmitters and their receptors, which are involved in the control of myelinating and non-myelinating glial cell ...
Roberta Piovesana +5 more
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Modulation of the M2 Muscarinic Acetylcholine Receptor Activity with Monoclonal Anti-M2 Receptor Antibody Fragments [PDF]
Journal of Biological Chemistry, 2004 Antibodies directed against the second extracellular loop of G protein-coupled receptors are known to have functional activities. From a partial agonist monoclonal antibody directed against the M2 muscarinic receptor, we constructed and produced a single chain variable fragment with high affinity for its target epitope.
Jean-Paul Briand +6 more
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Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist [PDF]
Nature, 2012 The parasympathetic branch of the autonomic nervous system regulates the activity of multiple organ systems. Muscarinic receptors are G-protein-coupled receptors that mediate the response to acetylcholine released from parasympathetic nerves. Their role in the unconscious regulation of organ and central nervous system function makes them potential ...
Haga, Kazuko +10 more
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Pronounced pharmacologic deficits in M2 muscarinic acetylcholine receptor knockout mice [PDF]
Proceedings of the National Academy of Sciences, 1999 Members of the muscarinic acetylcholine receptor family (M1–M5) are known to be involved in a great number of important central and peripheral physiological and pathophysiological processes. Because of the overlapping expression patterns of the M1–M5 muscarinic receptor subtypes and the lack of ligands endowed with sufficient subtype selectivity ...
Jesus Gomeza +8 more
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Desensitization and Internalization of the m2 Muscarinic Acetylcholine Receptor Are Directed by Independent Mechanisms [PDF]
Journal of Biological Chemistry, 1995 The phenomenon of acute desensitization of G-protein-coupled receptors has been associated with several events, including receptor phosphorylation, loss of high affinity agonist binding, receptor:G-protein uncoupling, and receptor internalization.
M. Marlene Hosey +4 more
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Tracking Voltage-Sensitive Movements of the M2 Muscarinic Acetylcholine Receptor [PDF]
Biophysical Journal, 2015 Numerous G protein-coupled receptors (GPCRs) have been shown to change their activity levels in response to changes in membrane potential. More recently, it has been directly demonstrated that these changes in activity reflect underlying changes in conformational state.
Francisco Bezanilla +4 more
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