Results 161 to 170 of about 5,131 (212)
Life Sciences, 1993 Knowledge of the distributions and functions of native m1-m5 muscarinic acetylcholine receptors in tissues is limited. To characterize the family of m1-m5 proteins directly, a panel of subtype-selective antibodies was generated against divergent i3 loop-fusion proteins. Each antibody was shown to bind a single cloned receptor specifically.
Allan I. Levey
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Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes
Neuron, 1988 The human and rat genes for a fifth muscarinic receptor have been cloned and expressed in mammalian cells. The 532 amino acid human protein has 89% sequence identity to the 531 amino acid rat protein and is most closely related to the m3 receptor. Both proteins are encoded by single exons.
Tom I. Bonner +3 more
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Characterization of methanthelinium binding and function at human M1–M5 muscarinic acetylcholine receptors [PDF]
Naunyn-Schmiedeberg's Archives of Pharmacology, 2018 Firstly, it was determined whether methanthelinium bromide (MB) binds to human M1-M5 (hM1-hM5) muscarinic acetylcholine receptors in comparison to the classical muscarinic antagonist N-methylscopolamine (NMS). [3H]NMS dissociation binding experiments revealed an allosteric retardation of dissociation at 100 μM of MB ranging from none in hM3 to 4.6-fold
Matthias Irmen +3 more
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Life Sciences, 2003 Until recently, little was known about the possible physiological functions of the M(5) muscarinic acetylcholine receptor subtype, the last member of the muscarinic receptor family (M(1)-M(5)) to be cloned. To learn more about the potential physiological roles of this receptor subtype, we generated and analyzed M(5) receptor-deficient mice (M5 -/- mice)
Masahisa Yamada +9 more
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Human skin fibroblasts express m2, m4, and m5 subtypes of muscarinic acetylcholine receptors
Journal of Cellular Biochemistry, 1999 Previous studies have demonstrated that muscarinic acetylcholine receptors (mAChRs) are expressed by human skin fibroblasts (HSF). We have identified the molecular subtypes of these receptors by reverse transcription-polymerase chain reaction (RT-PCR), using m1-m5 subtype-specific primers.
Rico Buchli +5 more
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Efficient coupling of m5 muscarinic acetylcholine receptors to activation of nitric oxide synthase.
The Journal of Pharmacology and Experimental Therapeutics, 1994 The coupling of m5 muscarinic acetylcholine receptors to the generation and release of nitric oxide (NO) was investigated. Chinese hamster ovary cells, which stably express m5 receptors, were transiently transfected with the gene encoding neuronal NO synthase and used as a model system. Increased generation of NO upon stimulation of cells by muscarinic
Shou Zhen Wang +2 more
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Molecular Pharmacology, 1994 Current models of the three-dimensional structures of muscarinic acetylcholine receptors and other G protein-coupled receptors are based primarily on high-resolution electron diffraction data obtained with bacteriorhodopsin, the molecular structure of which is characterized by the presence of seven alpha-helical transmembrane domains (TM I-VII ...
Zipora Pittel, J. Wess
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Neurochemical Research, 2003 Lymphocytes possess an independent, non-neuronal cholinergic system. Moreover, both T- and B-lymphocytes express multiple muscarinic acetylcholine receptors (mAChR). To obtain a better understanding of the regulatory mechanisms governing mAChR gene expression in the lymphocytic cholinergic system, we examined the effects of lymphocyte activation on ...
Takeshi Fujii +3 more
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