Results 161 to 170 of about 5,170 (209)
Life Sciences, 1993 Knowledge of the distributions and functions of native m1-m5 muscarinic acetylcholine receptors in tissues is limited. To characterize the family of m1-m5 proteins directly, a panel of subtype-selective antibodies was generated against divergent i3 loop-fusion proteins. Each antibody was shown to bind a single cloned receptor specifically.
Allan I. Levey
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Life Sciences, 2003 Until recently, little was known about the possible physiological functions of the M(5) muscarinic acetylcholine receptor subtype, the last member of the muscarinic receptor family (M(1)-M(5)) to be cloned. To learn more about the potential physiological roles of this receptor subtype, we generated and analyzed M(5) receptor-deficient mice (M5 -/- mice)
Masahisa Yamada +9 more
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Naunyn-Schmiedeberg's Archives of Pharmacology, 2018 Firstly, it was determined whether methanthelinium bromide (MB) binds to human M1-M5 (hM1-hM5) muscarinic acetylcholine receptors in comparison to the classical muscarinic antagonist N-methylscopolamine (NMS). [3H]NMS dissociation binding experiments revealed an allosteric retardation of dissociation at 100 μM of MB ranging from none in hM3 to 4.6-fold
Matthias Irmen +3 more
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P2‐207: Discovery of a highly M5‐preferring muscarinic acetylcholine receptor allosteric potentiator [PDF]
Alzheimer's & Dementia, 2009 Thomas M. Bridges +6 more
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Molecular Pharmacology, 1994 Current models of the three-dimensional structures of muscarinic acetylcholine receptors and other G protein-coupled receptors are based primarily on high-resolution electron diffraction data obtained with bacteriorhodopsin, the molecular structure of which is characterized by the presence of seven alpha-helical transmembrane domains (TM I-VII ...
Zipora Pittel, J. Wess
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Efficient coupling of m5 muscarinic acetylcholine receptors to activation of nitric oxide synthase.
The Journal of Pharmacology and Experimental Therapeutics, 1994 The coupling of m5 muscarinic acetylcholine receptors to the generation and release of nitric oxide (NO) was investigated. Chinese hamster ovary cells, which stably express m5 receptors, were transiently transfected with the gene encoding neuronal NO synthase and used as a model system. Increased generation of NO upon stimulation of cells by muscarinic
Shou Zhen Wang +2 more
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Psychopharmacology, 2007 Schizophrenic patients show decreased measures of sensorimotor gating, such as prepulse inhibition of startle (PPI). In preclinical models, these measures may be used to predict antipsychotic activity. While current antipsychotic drugs act largely at dopamine receptors, the muscarinic acetylcholine receptors offer promising novel pharmacotherapy ...
Morgane Thomsen +5 more
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Functional expression of rat M5 muscarinic acetylcholine receptor in yeast
Biochemical and Biophysical Research Communications, 1992 We have produced the rat M5 muscarinic acetylcholine receptor, an integral membrane protein, in the yeast Saccharomyces cerevisiae. This was achieved by placing an M5 gene in the yeast vector under the control of the yeast alpha-factor promoter and leader sequence.
Hao‐Jen Huang +3 more
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Neurochemical Research, 2003 Lymphocytes possess an independent, non-neuronal cholinergic system. Moreover, both T- and B-lymphocytes express multiple muscarinic acetylcholine receptors (mAChR). To obtain a better understanding of the regulatory mechanisms governing mAChR gene expression in the lymphocytic cholinergic system, we examined the effects of lymphocyte activation on ...
Takeshi Fujii +3 more
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Human skin fibroblasts express m2, m4, and m5 subtypes of muscarinic acetylcholine receptors
Journal of Cellular Biochemistry, 1999 Previous studies have demonstrated that muscarinic acetylcholine receptors (mAChRs) are expressed by human skin fibroblasts (HSF). We have identified the molecular subtypes of these receptors by reverse transcription-polymerase chain reaction (RT-PCR), using m1-m5 subtype-specific primers.
Rico Buchli +5 more
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