Results 171 to 180 of about 5,131 (212)
Functional expression of rat M5 muscarinic acetylcholine receptor in yeast
Biochemical and Biophysical Research Communications, 1992 We have produced the rat M5 muscarinic acetylcholine receptor, an integral membrane protein, in the yeast Saccharomyces cerevisiae. This was achieved by placing an M5 gene in the yeast vector under the control of the yeast alpha-factor promoter and leader sequence.
Hao‐Jen Huang +3 more
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Psychopharmacology, 2007 Schizophrenic patients show decreased measures of sensorimotor gating, such as prepulse inhibition of startle (PPI). In preclinical models, these measures may be used to predict antipsychotic activity. While current antipsychotic drugs act largely at dopamine receptors, the muscarinic acetylcholine receptors offer promising novel pharmacotherapy ...
Morgane Thomsen +5 more
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The Journal of Pharmacology and Experimental Therapeutics, 1995 Five subtypes of muscarinic acetylcholine receptors (m1-m5) have been expressed in insect cells (Spodoptera frugiperda, Sf9) using the baculovirus system. Up to 6 nmol of muscarinic acetylcholine receptors were produced by 1 liter culture; 0.3 to 0.6 (human m1), 3 to 6 (human m2), 2 to 4 (rat m3), 1 to 2 (rat m4) and 0.5 to 1 (human m5) nmol ...
Guanzhong Dong +3 more
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European Journal of Pharmacology, 1996 Based on the ability of many receptors to amplify NIH 3T3 cells, we developed a high throughput assay of cloned receptor pharmacology. In this assay, receptors are transiently co-expressed with the marker enzyme beta-galactosidase. Receptors that induce cellular proliferation select and amplify the cells that also express the marker, thus the ability ...
Hans Bräuner‐Osborne, Mark R. Brann
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The Journal of Pharmacology and Experimental Therapeutics, 1995 The neuromuscular blocking drug alcuronium was found earlier to increase the affinity of muscarinic receptors for methyl-N-scopolamine (NMS). This effect could be observed in some but not in other tissues. Subtype selectivity of the positive allosteric action of alcuronium was now investigated in radioligand binding experiments in Chinese hamster ovary
Ján Jakubík +3 more
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Journal of Cellular Physiology, 2018 AbstractBasal forebrain neurons control cerebral blood flow (CBF) by releasing acetylcholine (Ach), which binds to endothelial muscarinic receptors to induce nitric (NO) release and vasodilation in intraparenchymal arterioles. Nevertheless, the mechanism whereby Ach stimulates human brain microvascular endothelial cells to produce NO is still unknown ...
Estella Zuccolo +10 more
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A Molecular Mechanism for Endocytic Recycling of the M5 Muscarinic Acetylcholine Receptor
, 2010 A thesis presented to the faculty of The Rockefeller University in partial fulfillment of the requirements for the degree of Doctor of ...
Jacob Bendor
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Life Sciences, 1992 The coding sequence of the rat m3, m4 and m5 subtypes of muscarinic acetylcholine receptor (mAChR) genes was amplified by the polymerase chain reaction (PCR), cloned, and expressed in the murine fibroblast (B82) cell line. Sequencing of the cloned genes revealed some nucleotide differences when compared with the DNA sequence published in the literature.
Kenichi Kashihara +4 more
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An Improved Orthosteric Antagonist of the M5 Muscarinic Acetylcholine Receptor
Synfacts, 2022 Dirk Trauner, Christopher J. Arp
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