Results 111 to 120 of about 28,635 (297)

Co-occurrence analysis of insertional mutagenesis data reveals cooperating oncogenes

, 2007
Motivation: Cancers are caused by an accumulation of multiple independent mutations that collectively deregulate cellular pathways, e.g. such as those regulating cell division and cell-death. The publicly available Retroviral Tagged Cancer Gene Database (
Uren, A., Bot, J., Anthony Uren, Uren, A. (author), Kool, J., Jaap Kool, Reinders, M., Kool, J. (author), Bot, J. (author), Jan Bot, De Ridder, J., Wessels, L. (author), Jeroen de Ridder, De Ridder, J. (author), Wessels, L., Reinders, M. (author), Marcel Reinders, Lodewyk Wessels   +17 more
core   +1 more source

Mouse genetic corneal disease resulting from transgenic insertional mutagenesis [PDF]

, 2004
Background/aims: To report the generation of a new mouse model for a genetically determined corneal abnormality that occurred in transgenesis experiments.
Seabra, M. C., Huxley, C., Ramalho, J. S., Gregory-Evans, K.   +3 more
core   +1 more source

Machine Learning‐Enhanced Random Matrix Theory Design for Human Immunodeficiency Virus Vaccine Development

Advanced Intelligent Discovery, EarlyView.
This study integrates random matrix theory (RMT) and principal component analysis (PCA) to improve the identification of correlated regions in HIV protein sequences for vaccine design. PCA validation enhances the reliability of RMT‐derived correlations, particularly in small‐sample, high‐dimensional datasets, enabling more accurate detection of ...
Mariyam Siddiqah, Muhammad Zulqarnain Zeb, Muhammad Husnain Zeb, Babar Shabbir   +3 more
wiley   +1 more source

High-throughput trapping of secretory pathway genes in mouse embryonic stem cells

, 2006
High-throughput gene trapping is a random approach for inducing insertional mutations across the mouse genome. This approach uses gene trap vectors that simultaneously inactivate and report the expression of the trapped gene at the insertion site, and ...
Hansen, J., von Melchner, H., Wurst, W., Schnütgen, Frank, Seisenberger, C., Floss, Thomas, Seisenberger, Claudia, Hansen, Jens, Ruiz, Patricia, Ruiz, P., Schnütgen, F., Hollatz, M., De-Zolt, Silke, De-Zolt, S., Floss, T., Hollatz, Melanie, Wurst, Wolfgang, Melchner, Harald von (Prof. Dr.)   +17 more
core   +1 more source

Zebrafish and CRISPR—A synergistic approach to decipher and cure human diseases

Animal Models and Experimental Medicine, EarlyView.
Zebrafish, with high genetic homology to humans, serves as a powerful vertebrate model for disease modeling and drug discovery. Integration of CRISPR/Cas9 technology enables precise genome editing, facilitating the development of translational models for human diseases.
Manikandan Sivaprakasam, Aashika Raagavi Jeanpierre, Salma Mohammed, Rajesh Srinivasan, Aman Kumar Mohanty   +4 more
wiley   +1 more source

Mechanisms of insertional mutagenesis in human genes causing genetic disease

, 1991
Examples of the insertion of < 10 bp of DNA sequence into human gene-coding regions causing genetic disease were collated in order to study the underlying causative mechanisms.
Cooper, David Neil, Krawczak, Michael
core   +1 more source

Precise targeted integration by a chimaeric transposase zinc-finger fusion protein [PDF]

, 2010
Transposons of the Tc1/mariner family have been used to integrate foreign DNA stably into the genome of a large variety of different cell types and organisms.
Amy L. Bednarz, Colloms, S.D., Xiaofeng Feng, Bednarz, A.L., Sean D. Colloms, Feng, X.   +5 more
core   +1 more source

Animal research in the UK: Regulation, implementation, welfare and development of new approach methodologies

Animal Models and Experimental Medicine, EarlyView.
Scientific research with animals in the UK is regulated by the Animals (Scientific Procedures) Act 1986 with the National Centre for the Replacement, Refinement and Reduction of Animals in Research providing support for best practice and facilitating development of new approach methodologies.
Ewan St. John Smith, James Bussell, Maggie Gentry, Elliot Lilley, Cathy Merry, Judy MacArthur Clark, William Reynolds   +6 more
wiley   +1 more source

Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex

Advanced NanoBiomed Research, EarlyView.
We developed an assay to distinguish cellular binding from internalization. Compatible with microscopy and high‐throughput screening, the method identifies ligand‐mediated uptake. Applying top candidates to a protein‐based DNA carrier enhanced transfection efficiency, providing a rational strategy to improve non‐viral gene delivery systems.
David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean‐Christophe Leroux   +5 more
wiley   +1 more source

Overexpression of kermit/dGIPC is associated with lethality in Drosophila melanogaster

Brazilian Journal of Medical and Biological Research, 2011
Insertional mutagenesis is an important tool for functional genomics in Drosophila melanogaster. The insertion site in the KG00562 mutant fly line has been mapped to the CG8709 (herein named DmLpin) locus and to the 3’ of kermit (also called dGIPC). This
G.B. Pereira, V. Valente, M.S. de Queiroz, M.C.B. Vianna, M.L. Paçó-Larson   +4 more
doaj   +2 more sources