Results 191 to 200 of about 465,278 (302)

Directed mutagenesis of large multi-subunit protein complexes by plasmid sub-fragmentation. [PDF]

Sci Rep
Beghiah A, Kaila VRI.
europepmc   +1 more source

Nanoparticle‐Mediated TIPE1 mRNA Delivery Enhances Paclitaxel Sensitivity in Triple‐Negative Breast Cancer by Modulating RAB7A Ubiquitination‐Associated Stability and Autophagy

Advanced Science, EarlyView.
TIPE1m NPs nanoparticles restore TIPE1 expression, promote RAB7A ubiquitination and degradation, suppress autophagic flux, and resensitize paclitaxel‐resistant triple‐negative breast cancer to therapy. ABSTRACT Acquired paclitaxel (PTX) resistance remains a major obstacle in triple‐negative breast cancer (TNBC) treatment.
Wei Hu, Qishuai Chen, Yan Ma, Yang Liu, Xianing Dong, Wenjuan Wei, Jianxin Du, Shusheng Qiu, Maojin Tian, Peiqing Zhao   +9 more
wiley   +1 more source

Validated CRISPR/Cas9 guide RNAs targeting neurodevelopmental genes in the tunicate Ciona robusta. [PDF]

Differentiation
Popsuj S, Kalsang T, Kim K, Drummond E, Manekar P, Munagapati P, Oleti M, Sato H, Vickery I, Gigante ED, Stolfi A.   +10 more
europepmc   +1 more source

Sonoselective Transfection of Glioma Endothelium

Advanced Science, EarlyView.
Delivering non‐viral, gene‐bearing nanoparticles with thiol‐decorated surfaces to gliomas with focused ultrasound yields transfection tropism for endothelial cells. From this platform, “sonoselective” transfection of glioma endothelium is achieved via incorporation of an endothelial cell‐specific promoter.
Anna C. Debski, Catherine M. Gorick, Victoria R. Breza, Jackson Tirrell, Krishan Perumal, Katherine M. Nowak, Ji Song, Natasha D. Sheybani, Richard J. Price   +8 more
wiley   +1 more source

<i>Sleeping Beauty</i> mutagenesis identifies <i>BACH2</i> and other regulators of CD8⁺ T-cell exhaustion, persistence in vivo, and CAR-T cell function under tumor-associated chronic antigen stimulation. [PDF]

J Immunother Cancer
Lee CJ, Larsson AT, Jubenville TA, Hudson WA, Seeman ZJ, Stewart C, Tsai AK, Burrack A, Kimball BL, Siegler EL, Vianzon VV, Nolan EE, Yang YL, Stehn CM, Gohl DM, Donovan M, Temiz NA, Popescu FE, Warming S, Seshagiri S, You Y, Riordan JD, Dupuy A, Moriarity B, Rogers L, Stromnes I, Kenderian S, Largaespada D.   +27 more
europepmc   +1 more source

Maternal Exercise Rescues Embryonic Osteogenesis Impaired due to POLG Mutation Through a Potential Apelin‐ATF4 Axis

Advanced Science, EarlyView.
Maternal exercise (ME) increases apelin abundance across maternal and fetal tissues and is associated with improved fetal osteogenesis under POLG mutation‐induced mitochondrial dysfunction. Apelin‐APJ signaling is linked to enhanced mitochondrial function, Akt phosphorylation, and ATF4‐RUNX2 association, supporting coordinated fetal bone remodeling ...
Song Ah Chae, Ryan C. Riddle, Zhihua Jiang, Chaeyoung Shin, Min Du, Jun Seok Son   +5 more
wiley   +1 more source

EMS mutation and SNP detection in intracellular <i>Wolbachia</i> genomes. [PDF]

mSystems
Penunuri G, Pepper-Tunick E, McBroome J, Corbett-Detig R, Russell SL.   +4 more
europepmc   +1 more source

circZNF148 Drives Glucose Metabolism Reprogramming to Enhance Metastasis and Immune Evasion via HK1 Stabilization in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
CircZNF148 stabilizes HK1 through deubiquitinase recruitment, thereby enhancing glycolysis and lactate production. Elevated lactate promotes PD‑L1 lactylation and membrane accumulation while suppressing CD8+ T‐cell cytotoxicity, collectively facilitating immune evasion and malignant progression in TNBC.
Yuhan Jin, Wenjing Zhao, Lei Wang, Jianing Wang, Keying Zhao, Dan Luo, Yifei Wang, Yuxia Yang, Jingze Yang, Yaming Li, Tong Chen, Dianwen Han, Zekun Wang, Xiaoyan Li, Bing Chen, Lijuan Wang, Yiran Liang, Qifeng Yang   +17 more
wiley   +1 more source

High-throughput mutational analysis of F₁-ATPase by integrated cell-free protein synthesis and single-molecule rotation assay. [PDF]

Protein Sci
Taguchi M, Oya T, Ueno H, Noji H.
europepmc   +1 more source

Metabolic Reprogramming in Glioblastoma Stem Cells Promotes Radiation Resistance Through a H3K18la/USP30/MBOAT2 Axis

Advanced Science, EarlyView.
LDHA‐driven lactate production in mesenchymal glioma stem cells promotes p300‐dependent H3K18 lactylation, activates USP30 transcription, and stabilizes MBOAT2 to remodel phosphatidylethanolamines toward ferroptosis‐resistant PE‐MUFA species. Disrupting this metabolic–epigenetic–lipid circuit enhances ferroptosis and sensitizes glioblastoma to ...
Zong Miao, Wei Gu, Yimin Ren, Chenfei Lu, Wanzhi Cai, Junnan Lu, Zhongyuan Bao, Yanan Zhou, Rong Li, Ke Jin, Chao Chen, Hongxiang Wang, Lei Xu, Juxiang Chen   +13 more
wiley   +1 more source