Results 211 to 220 of about 664,651 (329)

Development of New Antimicrobial Peptides by Directional Selection. [PDF]

Antibiotics (Basel)
Grafskaia E, Bobrovsky P, Kharlampieva D, Brovina K, Serebrennikova M, Alieva S, Selezneva O, Bessonova E, Lazarev V, Manuvera V.   +9 more
europepmc   +1 more source

USP35 Acts as a Deubiquitinating Enzyme for ID3 to Promote Immune Escape in Colorectal Cancer

Advanced Science, EarlyView.
USP35 stabilizes ID3 expression by deubiquitinating the K2/K30 site, thereby upregulating PD‐L1 and promoting immune escape in colorectal cancer. IU1, an inhibitor of USP35 enzyme activity, has been shown to inhibit USP35, thereby accelerating ID3 degradation, enhancing CD8+ T cell killing, and reversing the immunosuppressive microenvironment ...
Wenxin Chen, Ling Wang, Hongmei Fan, Lisheng Li, Lingyu Zhang, Ruoxin Li, Fang Liu, Fuli Wen, Yunbin Ye, Chuanzhong Huang   +9 more
wiley   +1 more source

Revisiting classical <i>Escherichia coli</i> cell division mutants by whole-genome sequencing. [PDF]

Microb Genom
Dahdouh E, García-Pérez I, Reyes-Zuñagua DS, Mingorance J, Vicente M.   +4 more
europepmc   +1 more source

T-DNA mutagenesis in Brachypodium distachyon [PDF]

, 2011
Vera Thole, Antoine Peraldi, B. Worland, P. Nicholson, John H. Doonan, Philippe Vain   +5 more
openalex   +1 more source

From Natural Discovery to AI‐Guided Design: A Curated Collection of Compact Enhancers for Crop Engineering

Advanced Science, EarlyView.
ABSTRACT Precise transgene‐free gene upregulation remains a challenge in crop biotechnology, as conventional enhancers often exceed CRISPR‐mediated knock‐in size constraints and face regulatory hurdles. Here we establish a foundational cross‐species resource of compact transcriptional enhancers developed via STEM‐seq, a high‐throughput screening ...
Qi Yao, Jin Gao, Keling Wang, Yufeng Liu, Peijin Han, Hong Pan, Xiaofeng Yang, Qianlan Yin, Dating Zhong, Lu Ye, Qi Deng, Lingling Gao, Xiaoyu Tu, Dequan Wang, Yuming Lu   +14 more
wiley   +1 more source

Genome-wide strand-specific UV mutagenesis in <i><i>Escherichia coli</i></i> is directed by the Mfd translocase. [PDF]

Proc Natl Acad Sci U S A
Adebali O, Mieczkowski PA, Sancar A, Selby CP.   +3 more
europepmc   +1 more source

2P282 Comparative analyses of activation mechanisms of rhodopsin using site-directed mutagenesis

, 2005
Hisao Tsukamoto, Akihisa Terakita, Y. Shichida   +2 more
openalex   +2 more sources

Structure‐Guided Engineering of a Cas12i Nuclease Unlocks Near‐PAMless Genome Editing

Advanced Science, EarlyView.
CRISPR‐Cas nucleases are limited by PAM requirements, restricting genome accessibility. Structure‐guided engineering of the compact Cas12i nuclease SF01 produced three variants with near‐PAMless, enabling efficient editing at diverse 5'‐NNTN‐3' sites. These nucleases expand the editable portion of the human genome more than fourfold, enabling efficient
Qitong Chen, Hanlin Gou, Chao Xu, Sihan Wang, Huitao Zhang, Minglei Song, Mengge Wang, Xingkun Ji, Xiaofei Wei, Yuanyan Tan, Hehua Quan, Pengyu Luo, Hanyu Shou, Pengpeng Liu, Yafeng Liang, Jian‐Kang Zhu   +15 more
wiley   +1 more source

SPIN-guided engineering of a novel (<i>R</i>)-amine transaminase from <i>Fusarium albosuccineum</i> for enantioselective synthesis of chiral piperidyl amines. [PDF]

Synth Syst Biotechnol
Tang R, Li J, Yang X, Tian X, Wang Z, Zhang X, Li T.   +6 more
europepmc   +1 more source

GPCRs in CAR‐T Cell Immunotherapy: Expanding the Target Landscape and Enhancing Therapeutic Efficacy

Advanced Science, EarlyView.
Chimeric antigen receptor T cell therapy faces dual challenges of target scarcity and an immunosuppressive microenvironment in solid tumors. This review highlights how G protein‐coupled receptors can serve as both novel targets to expand the therapeutic scope and functional modules to enhance CAR‐T cell efficacy.
Zhuoqun Liu, Yuchen Xiao, Yamin Zhao, Lihe Dai, David J.H. Shih, Shikang Liang, Honglei Tian, Liu Liu, Feng Tian, Bing Liu, Lei Chang, Chao Zhang   +11 more
wiley   +1 more source