Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing. [PDF]
Price D +8 more
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Correction to "Reduced Susceptibility to Phytophthora in Non-Transgenic Cacao Progeny Through CRISPR-Cas9 Mediated TcNPR3 Mutagenesis". [PDF]
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PrimerWeaver: an integrated web server for primer design in molecular biology workflows. [PDF]
Jin Z, Kim Y, Ignea C.
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Engineering the L-Arabinose Isomerase from <i>Lactobacillus sakei</i> for Whole-Cell Conversion Production of D-Tagatose Under High Substrate Concentration Conditions. [PDF]
He R, Liu W, Tao B, Liang S, Tang X.
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Effect of ethyl methane sulfonate mutagenesis on phenological, yield-related and yield traits in cowpea (Vigna unguiculata (L.) Walp). [PDF]
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A targeted mutational strategy aiding in generating antisense RNA to knockdown the <i>Ehrlichia chaffeensis</i> P28-outer membrane protein 19 expression. [PDF]
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In Escherichia coli, UV and many chemicals appear to cause mutagenesis by a process of translesion synthesis that requires some form of DNA polymerase III and the SOS-regulated proteins UmuD, UmuC and RecA. An analysis of SOS mutagenesis offers insights into the molecular basis of induced mutagenesis and into mechanisms of DNA damage tolerance.
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Mutagenesis and TILLING to Dissect Gene Function in Plants [PDF]
Mutagenesis can be random or targeted and occur by nature or artificially by humans. However, the bulk of mutagenesis employed in plants are random and caused by physical agents such as x-ray and gamma-ray or chemicals such as ethyl-methane sulfonate ...
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To identify new genes in an organism, a genetic approach can be used to screen for mutations that display a particular phenotype. Genotoxic agents, such as ultraviolet (UV) light, ionizing radiation, or chemicals can be used to randomly induce DNA lesions in the genome.
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