Results 251 to 260 of about 470,712 (310)
Some of the next articles are maybe not open access.
Annual Review of Biochemistry, 1999
▪ Abstract Enzymatic hydrolysis of glycosides can occur by one of two elementary mechanisms identified by the stereochemical outcome of the reaction, inversion or retention. The key active-site residues involved are a pair of carboxylic acids in each case, and strategies for their identification and for probing the details of their roles in catalysis
H D, Ly, S G, Withers
openaire +2 more sources
▪ Abstract Enzymatic hydrolysis of glycosides can occur by one of two elementary mechanisms identified by the stereochemical outcome of the reaction, inversion or retention. The key active-site residues involved are a pair of carboxylic acids in each case, and strategies for their identification and for probing the details of their roles in catalysis
H D, Ly, S G, Withers
openaire +2 more sources
Biological Reviews, 1949
SummaryThe general methodological requirements for work with chemical mutagens are the same as for general mutation work, with special emphasis on questions of concentration, penetration, possible indirect or delayed effect, differences in susceptibility between individuals, strains and species.
openaire +2 more sources
SummaryThe general methodological requirements for work with chemical mutagens are the same as for general mutation work, with special emphasis on questions of concentration, penetration, possible indirect or delayed effect, differences in susceptibility between individuals, strains and species.
openaire +2 more sources
Methods, 2004
Mutagenesis represents a powerful methodology for the analysis of protein structural and functional relationships and dissection of complex protein-protein interactions. The suicide substrate-like inhibitory mechanism of the proteins of the serpin superfamily offers unique challenges for the design of mutagenesis studies.
Toni M, Antalis, Daniel A, Lawrence
openaire +2 more sources
Mutagenesis represents a powerful methodology for the analysis of protein structural and functional relationships and dissection of complex protein-protein interactions. The suicide substrate-like inhibitory mechanism of the proteins of the serpin superfamily offers unique challenges for the design of mutagenesis studies.
Toni M, Antalis, Daniel A, Lawrence
openaire +2 more sources
Annual Review of Nutrition, 2008
Diet-related mutagenesis plays an etiologic role in chronic diseases, including cardiovascular disease and cancer. Many dietary mutagens are DNA reactive, leading to distinct spectra of base-pair substitution mutations and structural chromosome changes.
Lynnette R, Ferguson, Martin, Philpott
openaire +2 more sources
Diet-related mutagenesis plays an etiologic role in chronic diseases, including cardiovascular disease and cancer. Many dietary mutagens are DNA reactive, leading to distinct spectra of base-pair substitution mutations and structural chromosome changes.
Lynnette R, Ferguson, Martin, Philpott
openaire +2 more sources
2014
Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we
openaire +2 more sources
Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we
openaire +2 more sources
2007
Ethyl methane sulfonate (EMS) mutagenesis in Arabidopsis is the most widely used mutagenesis technique. EMS has high mutagenicity and low mortality and can be used in any laboratory with a fume hood. The chemical principle of EMS mutagenesis is simple; it is based on the ability of EMS to alkylate guanine bases, which results in base mispairing.
Jodi, Maple, Simon G, Møller
openaire +2 more sources
Ethyl methane sulfonate (EMS) mutagenesis in Arabidopsis is the most widely used mutagenesis technique. EMS has high mutagenicity and low mortality and can be used in any laboratory with a fume hood. The chemical principle of EMS mutagenesis is simple; it is based on the ability of EMS to alkylate guanine bases, which results in base mispairing.
Jodi, Maple, Simon G, Møller
openaire +2 more sources
2007
Forward genetics in Drosophila has profoundly affected our understanding of circadian rhythms in this organism and, more generally, in the animal kingdom. Most Drosophila pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies.
openaire +3 more sources
Forward genetics in Drosophila has profoundly affected our understanding of circadian rhythms in this organism and, more generally, in the animal kingdom. Most Drosophila pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies.
openaire +3 more sources
Environmental Mutagenesis, 1981
AbstractI would first like to thank the Environmental Mutagen Society for honoring me with its 1980 award; I also would like to take this opportunity to acknowledge all the people who have worked with me and helped me since I started my work in mutagenesis in 1953.
openaire +2 more sources
AbstractI would first like to thank the Environmental Mutagen Society for honoring me with its 1980 award; I also would like to take this opportunity to acknowledge all the people who have worked with me and helped me since I started my work in mutagenesis in 1953.
openaire +2 more sources
Programmed Mutagenesis Is Universal
Theory of Computing Systems, 2002zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Julia Khodor, David K. Gifford
openaire +2 more sources
2007
Localized mutagenesis can be used to obtain mutants in genes of interest based on linkage to selectable markers. Mutagens diethylsulfate and hydroxylamine are used to obtain predominantly transition mutations in the DNA either by whole chromosomal mutagenesis or mutagenesis of DNA isolated as purified plasmid or packaged in transducing phage particles.
openaire +2 more sources
Localized mutagenesis can be used to obtain mutants in genes of interest based on linkage to selectable markers. Mutagens diethylsulfate and hydroxylamine are used to obtain predominantly transition mutations in the DNA either by whole chromosomal mutagenesis or mutagenesis of DNA isolated as purified plasmid or packaged in transducing phage particles.
openaire +2 more sources