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The Biochemistry of Mutagenesis
Annual Review of Biochemistry, 1976Mutagenesis has remained an intriguing aspect of genetics since the beginning of this century, and its analysis has proceeded hand in hand with the elucidation of gene replication and expression. Interest in this area has further heightened with the growing awareness that numerous environmental agents may cause mutations in humans.
J W, Drake, R H, Baltz
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Annual Review of Genetics, 1985
TOTAL SYNTHESIS OF MUTANTS . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 Solid Phase Chemical Synthesis of Oligodeoxyribonucleotides .. . ..... 424 Assembly of Oligodeoxyribonucleotides into Double-Strand DNA 427 Mutation by Partial Gene Synthesis (Cassette Mutagenesis)
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TOTAL SYNTHESIS OF MUTANTS . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424 Solid Phase Chemical Synthesis of Oligodeoxyribonucleotides .. . ..... 424 Assembly of Oligodeoxyribonucleotides into Double-Strand DNA 427 Mutation by Partial Gene Synthesis (Cassette Mutagenesis)
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Environmental and Molecular Mutagenesis, 1989
Our understanding of mechanisms of mutation, which has expanded greatly in the past few decades, served as the original impetus to the formation of the Environmental Mutagen Society. The advances in genetics and chemistry that have conditioned our present degree of knowledge are here catalogued and the future is predicted.
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Our understanding of mechanisms of mutation, which has expanded greatly in the past few decades, served as the original impetus to the formation of the Environmental Mutagen Society. The advances in genetics and chemistry that have conditioned our present degree of knowledge are here catalogued and the future is predicted.
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Annual Review of Biochemistry, 1999
▪ Abstract Enzymatic hydrolysis of glycosides can occur by one of two elementary mechanisms identified by the stereochemical outcome of the reaction, inversion or retention. The key active-site residues involved are a pair of carboxylic acids in each case, and strategies for their identification and for probing the details of their roles in catalysis
H D, Ly, S G, Withers
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▪ Abstract Enzymatic hydrolysis of glycosides can occur by one of two elementary mechanisms identified by the stereochemical outcome of the reaction, inversion or retention. The key active-site residues involved are a pair of carboxylic acids in each case, and strategies for their identification and for probing the details of their roles in catalysis
H D, Ly, S G, Withers
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Biological Reviews, 1949
SummaryThe general methodological requirements for work with chemical mutagens are the same as for general mutation work, with special emphasis on questions of concentration, penetration, possible indirect or delayed effect, differences in susceptibility between individuals, strains and species.
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SummaryThe general methodological requirements for work with chemical mutagens are the same as for general mutation work, with special emphasis on questions of concentration, penetration, possible indirect or delayed effect, differences in susceptibility between individuals, strains and species.
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Methods, 2004
Mutagenesis represents a powerful methodology for the analysis of protein structural and functional relationships and dissection of complex protein-protein interactions. The suicide substrate-like inhibitory mechanism of the proteins of the serpin superfamily offers unique challenges for the design of mutagenesis studies.
Toni M, Antalis, Daniel A, Lawrence
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Mutagenesis represents a powerful methodology for the analysis of protein structural and functional relationships and dissection of complex protein-protein interactions. The suicide substrate-like inhibitory mechanism of the proteins of the serpin superfamily offers unique challenges for the design of mutagenesis studies.
Toni M, Antalis, Daniel A, Lawrence
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Annual Review of Nutrition, 2008
Diet-related mutagenesis plays an etiologic role in chronic diseases, including cardiovascular disease and cancer. Many dietary mutagens are DNA reactive, leading to distinct spectra of base-pair substitution mutations and structural chromosome changes.
Lynnette R, Ferguson, Martin, Philpott
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Diet-related mutagenesis plays an etiologic role in chronic diseases, including cardiovascular disease and cancer. Many dietary mutagens are DNA reactive, leading to distinct spectra of base-pair substitution mutations and structural chromosome changes.
Lynnette R, Ferguson, Martin, Philpott
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2014
Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we
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Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we
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2007
Ethyl methane sulfonate (EMS) mutagenesis in Arabidopsis is the most widely used mutagenesis technique. EMS has high mutagenicity and low mortality and can be used in any laboratory with a fume hood. The chemical principle of EMS mutagenesis is simple; it is based on the ability of EMS to alkylate guanine bases, which results in base mispairing.
Jodi, Maple, Simon G, Møller
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Ethyl methane sulfonate (EMS) mutagenesis in Arabidopsis is the most widely used mutagenesis technique. EMS has high mutagenicity and low mortality and can be used in any laboratory with a fume hood. The chemical principle of EMS mutagenesis is simple; it is based on the ability of EMS to alkylate guanine bases, which results in base mispairing.
Jodi, Maple, Simon G, Møller
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2007
Forward genetics in Drosophila has profoundly affected our understanding of circadian rhythms in this organism and, more generally, in the animal kingdom. Most Drosophila pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies.
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Forward genetics in Drosophila has profoundly affected our understanding of circadian rhythms in this organism and, more generally, in the animal kingdom. Most Drosophila pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies.
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