Results 261 to 270 of about 664,651 (329)

Opposing fates: a bipolar cellular model for FMDV replication shaped by ¹²C⁶⁺heavy-ion mutagenesis. [PDF]

Front Cell Infect Microbiol
Song X, Cui Y, An F, Li Y, Liang J, Tao S, Liu X.   +6 more
europepmc   +1 more source

An Expanded Toolbox for Versatile Chemical Editing of Adeno‐Associated Virus

Angewandte Chemie, EarlyView.
We describe technology to introduce diverse non‐natural chemical functionalities site‐specifically into the capsid of adeno‐associated virus through genetic code expansion, and using them to engineer this leading vector for human gene therapy for enhanced tissue specificity and reduced immunogenicity Abstract Site‐specific incorporation of noncanonical
Quan Pham, Jake Glicksman, Boyang Han, David Koo, Conor Loynd, Soumya Jyoti Singha Roy, Abhishek Chatterjee   +6 more
wiley   +2 more sources

Genetic analysis of pigment production in the fungus Exophiala dermatitidis mutant strains obtained via nontargeted UV mutagenesis. [PDF]

G3 (Bethesda)
Chhoker K, Hausner G, Harris SD.
europepmc   +1 more source

Optimization of gamma irradiation doses (60Co) for mutagenesis in strawberry (Fragaria × annanasa Duch) cv. Winter Dawn

Rahul R. Rodge, Rajni Rajan, Kuldeep Pandey, Harjinder Kaur, Khan Jabroot, Madhubanti Chaudhuri, Jyoti Sharma, Sunny Sharma, N. M. Patil, AP Wagh, D. N. Rank, A. A. Kardak   +11 more
openalex   +2 more sources

Co‐Opting MBNL‐Dependent Alternative Splicing Cassette Exons to Control Gene Therapy in Myotonic Dystrophy

Annals of Neurology, EarlyView.
Objective Myotonic dystrophy type 1 (DM1) is a highly variable, multisystemic genetic disorder caused by a CTG repeat expansion in the 3′ untranslated region of DMPK. Toxicity is exerted by repeat‐containing DMPK transcripts that sequester muscleblind‐like (MBNL) proteins and lead to deleterious yet predictable changes in alternative splicing.
Samuel T. Carrell, Ellie M. Carrell, Ryan Giovenco, Beverly L. Davidson   +3 more
wiley   +1 more source

Unnatural Amino Acid and Emerging Chemistry Approaches to Map RNA–Protein Interactions

Angewandte Chemie, EarlyView.
This review highlights emerging chemistries for mapping RNA–protein interactions, including genetically encoded unnatural amino acids, novel photocrosslinkers, and non‐photoactivatable crosslinking systems. We compare their mechanisms, reactivity and applications, outlining how these next‐generation tools enable higher‐resolution, site‐specific ...
Eryn Lundrigan, Parrish Evers, Elexa Scott, John Paul Pezacki   +3 more
wiley   +2 more sources

Callus and endosperm green fluorescence reporter-assisted selection system in maize CRISPR/Cas9 gene editing. [PDF]

BMC Plant Biol
Ge L, Li W, Dou Y, Ma Y, Sun M, Chen X, Feng X, Li Y, Yu Q.   +8 more
europepmc   +1 more source

In vivo characterization of the lysine-methyltransferases Set7/9 and G9a by conditional mutagenesis in the mouse

, 2009
Bernhard Lehnertz
openalex   +1 more source

Enhanced Sensitivity of a Modified Quaking‐Induced Conversion Diagnostic Test for the Broad Detection of Sporadic and Inherited Prion Diseases: A Retrospective Study

Annals of Neurology, EarlyView.
Objective Quaking‐induced conversion (QuIC) tests, which detect prion‐seeding activity in cerebrospinal fluid (CSF), have markedly advanced the antemortem diagnosis of prion diseases such as Creutzfeldt‐Jakob disease (CJD). These tests provide high diagnostic accuracy and enable timely differentiation from other rapidly progressive neurodegenerative ...
Jennifer Myskiw, Rebecca Fox, Dominic M.S. Kielich, Lise Lamoureux, Melanie Leonhardt, Olivia Nykvist, Jessy A. Slota, Kristen Avery, Clark Phillipson, Kathy Frost, Sharon Simon, Brian S. Appleby, Ben A. Bailey‐Elkin, Stephanie A. Booth   +13 more
wiley   +1 more source

Engineering Fluoroacetate Dehalogenase by Growth‐Based Selections on Non‐Natural Organofluorides

Angewandte Chemie, EarlyView.
We present a high‐throughput selection system to engineer fluoroacetate dehalogenases (FAcDs). By challenging E. coli populations that produce diverse FAcD libraries to grow on non‐natural organofluorides as their sole carbon source, we isolated a panel of FAcD variants with improved activity and altered substrate specificity.
Suzanne C. Jansen, Pauline van Beers, Clemens Mayer   +2 more
wiley   +2 more sources