Results 271 to 280 of about 664,651 (329)

High-throughput discovery of transmembrane helix dimers from human single-pass membrane proteins with TOXGREEN sort-seq. [PDF]

PNAS Nexus
Anderson SM, Choi J, Cushman EM, Leander M, Raman S, Senes A.   +5 more
europepmc   +1 more source

Single-stranded DNA (ssDNA) donor repair templates and CRISPR/Cas9 enable a high-frequency of targeted insertions in potato. [PDF]

Front Genome Ed
González MN, Salaria N, Friberg M, Liu Y, Alverup J, Andersson M, Hofvander P.   +6 more
europepmc   +1 more source

Mutagenesis of nifE and nifN from Azotobacter vinelandii

, 1988
Mark Steven Michael Wilson
openalex   +1 more source

Identification of determinants of high-fidelity DNA synthesis in Mycobacterium smegmatis DnaE1 through in silico and in vivo approaches. [PDF]

Nucleic Acids Res
Kuin RCM, van Westen GJP, Lamers MH.
europepmc   +1 more source

TILLCANN: a TILLING platform in Cannabis sativa for mutation discovery and crop improvement. [PDF]

Mol Hortic
Duarte-Delgado D, Alexiou KG, Pujol M, Uauy C, Adamski NM, Vidal V, Torres A, Zalewski C, Gaudino R, Monfort A, Argyris J.   +10 more
europepmc   +1 more source

Nonheme Fe Enzyme‐Catalyzed Enantiodivergent Nitrogen Migration: Directed Evolution and Computational Study of Isopenicillin N Synthases for Biocatalytic Synthesis of Arylglycines

Angewandte Chemie, EarlyView.
Nonheme Fe enzyme isopenicillin N synthase was reprogrammed and evolved as an efficient nitrogen migratase IPNSNim, converting diverse azanyl esters to valuable l‐arylglycines with up to 16 000 TTN and 97:3 e.r. IPNSNim and ACCONim allowed enantiodivergent preparation of both l‐ and d‐arylglycines.
Ken Lin, Liu‐Peng Zhao, Shengchun Wang, Huichong Liu, Yu Zhang, Binh Khanh Mai, Peng Liu, Yang Yang   +7 more
wiley   +1 more source

Mutations in ribosomal protein uS5 alter translation fidelity and mutagenesis in <i>Pseudomonas putida</i>. [PDF]

J Bacteriol
Jürgenstein K, Ilves H, Luhaäär C, Brauer A, Remme J, Kivisaar M.   +5 more
europepmc   +1 more source

Discovery of a First‐in‐Class Covalent Allosteric SHP1 Inhibitor with Immunotherapeutic Activity

Angewandte Chemie, EarlyView.
A covalent allosteric inhibitor M029 was discovered for SHP1, a novel target for immunotherapy. M029 binds to a cryptic Cys remote from the active site, exhibits robust target engagement, and blocks tumor progression by stimulating antitumor immune response.
Zihan Qu, Frederick Nguele Meke, Zheng Zhang, Aaron D. Krabill, Christine S. Muli, Brenson A. Jassim, Jiajun Dong, Quyen D. Nguyen, Yunpeng Bai, Jinyue Li, Yiming Miao, Bardia Asadi, Levi M. Johnson, Jinmin Miao, Darci J. Trader, W. Andy Tao, Zhong‐Yin Zhang   +16 more
wiley   +1 more source

Structural Analysis of Missense Mutations on the Stability of APOE3 and APOE4. [PDF]

Genes (Basel)
Anthony M, Xie Y, O'Neil JN, Teng S.
europepmc   +1 more source

PCA‐Based Database Mining Enables the Discovery of Bacterial Carbene Transferases for Stereodivergent Cyclopropanation

Angewandte Chemie, EarlyView.
Database mining using principal component analysis (PCA)‐based clustering enabled discovery of bacterial enzymes capable of catalyzing stereodivergent cyclopropanation of styrene. Statistical analyses of sequence data further revealed characteristic structural properties of these enzymes, highlighting the potential of the bioinformatics tools for ...
Shunsuke Kato, Koki Takeuchi, Kohei Umeda, Hisashi Kudo, Tomohisa Hasunuma, Takashi Hayashi   +5 more
wiley   +1 more source