Results 81 to 90 of about 462,704 (302)

A Microcapsule‐Integrated smRandom‐seq Platform Enables Fixation‐Free Single Microbe RNA Sequencing

Advanced Science, EarlyView.
We developed a microcapsule‐integrated high‐throughput fixation‐free single‐microbe RNA‐seq workflow in which microbes are encapsulated in aqueous two‐phase microcapsules for physical confinement of nucleic acids. This platform enables co‐encapsulation with barcoded beads for sequencing and reveals that fixation‐free processing enhances transcriptomic ...
Mengdi Song, Yuting Wang, Zhaolun Wang, Shunji Zhang, Zhengmin Tang, Yongcheng Wang   +5 more
wiley   +1 more source

Improved recovery of Mycobacterium tuberculosis from children using the microscopic observation drug susceptibility method.

, 2006
OBJECTIVE: The diagnosis of pulmonary tuberculosis presents challenges in children, because symptoms are nonspecific, sputa are not accessible, and Mycobacterium tuberculosis cultures and smears often are negative.
Luz Caviedes, Oberhelman, Richard A, Gilman, Robert H, Giselle Soto-Castellares, Carlton Evans, Castillo, Maria E, Robert H. Gilman, Moore, David AJ, David A.J. Moore, Maria E. Castillo, Patricia Kissinger, Richard A. Oberhelman, Caviedes, Luz, Evans, Carlton, Soto-Castellares, Giselle, Kissinger, Patricia   +15 more
core   +1 more source

Distribution and transmission of mycobacterium tuberculosis complex lineages among children in peri-urban Kampala, Uganda [PDF]

, 2015
To gain insight into the transmission of tuberculosis (TB) in peri-urban Kampala-Uganda, we performed a household contact study using children as a surrogate for recent transmission of Mycobacterium tuberculosis (MTB).
Eddie M. Wampande, Kathleen Eisenach, Mary Nsereko, Wampande, Eddie M., W. Henry Boom, Mayanja, Harriet K., Mupere, Ezekiel, Gagneux, Sebastien, Eisenach, Kathleen, Joloba, Moses L., Moses L. Joloba, Ezekiel Mupere, Nsereko, Mary, Sebastien Gagneux, Harriet K. Mayanja, null null, Devan Jaganath, Boom, W. Henry, Jaganath, Devan, Tuberculosis Research Unit,   +19 more
core   +2 more sources

Upadacitinib Restrains the Pathogenic Fitness of CD4+ T Cells and Aberrant B Cell Programming in Optic Neuritis

Advanced Science, EarlyView.
Single‐cell profiling and functional perturbation reveal coordinated JAK1‐pSTAT3 downstream programs in optic neuritis, including MCL1‐dependent fitness of pathogenic CD4+ Tem cells and glycolysis‐linked, cholesterol‐sensitive B‐cell responses associated with RORA. Upadacitinib disrupts this reciprocal T‐B‐cell circuit and alleviates neuroinflammation,
Gengchen Jiang, Qi Jiang, Lian Han, Yue Peng, Xingyu Liu, Tong Yang, Hao Huang, Jiaying Liu, Yuan Gao, Hui Yang, Wenjun Zou, Zhaohuai Li, Wenru Su   +12 more
wiley   +1 more source

Tetrahydroisoquinolines affect the whole-cell phenotype of Mycobacterium tuberculosis by inhibiting the ATP-dependent MurE ligase. [PDF]

, 2015
(S)-Leucoxine, isolated from the Colombian Lauraceae tree Rhodostemonodaphne crenaticupula Madriñan, was found to inhibit the growth of Mycobacterium tuberculosis H37Rv.
Mortazavi, Parisa Nakhostin, Munshi, T, Bhakta, S, Maitra, A, Cuca, L.E., Pesnot, T, Pesnot, T., Hailes, HC, Angell, R, Barrera, DA, Needham, D, Hailes, H.C., Bhakta, Sanjib, Davies, H.M., Gershater, MC, Davies, HM, Barrera, D.A., Evangelopoulos, Dimitrios, Cuca, LE, McMahon, E, Maitra, Arundhati, Redmond, J.M., Ward, J.M., Evangelopoulos, D, Mortazavi, PN, Munshi, Tulika, Gershater, M.C., Ward, JM, Lamming, E.D., Redmond, JM, McMahon, E., Angell, R., Guzman, JD, Guzman, Juan D., Needham, D., Lamming, ED   +35 more
core   +1 more source

Accelerating Primary Screening of USP8 Inhibitors from Drug Repurposing Databases with Tree‐Based Machine Learning

Advanced Intelligent Discovery, EarlyView.
This study introduces a tree‐based machine learning approach to accelerate USP8 inhibitor discovery. The best‐performing model identified 100 high‐confidence repurposable compounds, half already approved or in clinical trials, and uncovered novel scaffolds not previously studied. These findings offer a solid foundation for rapid experimental follow‐up,
Yik Kwong Ng, Sharon Ai Er Lam, Wei Chun Lim, Hannah Hui Min Toh, Shi Jun Ang   +4 more
wiley   +1 more source

Two Years of Ocrelizumab Treatment in Black and Hispanic People with Multiple Sclerosis in CHIMES: A Single‐Arm Clinical Trial

Annals of Neurology, EarlyView.
Objective To evaluate the effectiveness and safety of ocrelizumab in self‐identified black and Hispanic people with relapsing multiple sclerosis. Methods The Characterization of Ocrelizumab in Minorities with Multiple Sclerosis (CHIMES) trial, a prospective, open‐label, single‐arm, phase 4 study, intentionally recruited underrepresented populations in ...
Lilyana Amezcua, Anthony T. Reder, Evanthia Bernitsas, Nancy L. Monson, Timothy Vartanian, Gregory F. Wu, Mansoor Saleh, Annette Okai, Ahmed Z. Obeidat, Dilraj Sokhi, Rikisha Parekh, Jinglan Pei, Christopher Harp, David Clayton, Ibraheem Abioye, Mitzi J. Williams, for the CHIMES Study Group   +16 more
wiley   +1 more source

Rewriting Tumor Immunity With Bacterial Membrane Vesicles: A Versatile and Potent Therapeutic Strategy

Advanced NanoBiomed Research, EarlyView.
Bacterial membrane vesicles (BMVs) emerge as promising platforms for cancer immunotherapy owing to their intrinsic adjuvant properties and tunable cargo delivery capabilities. Their ability to modulate the tumor microenvironment, enhance antitumor immune responses, and support personalized therapeutic strategies highlights their growing potential as ...
Md Sifat Rahi, Md Mijanur Rahman, Asma Talukder, Urooj Yousaf Virk, Jennifer C. Wilson, Nigel McMillan, Ming Q. Wei   +6 more
wiley   +1 more source

Acute and persistent Mycobacterium tuberculosis infections depend on the thiol peroxidase TpX. [PDF]

, 2009
The macrophage is the natural niche of Mycobacterium tuberculosis infection. In order to combat oxidative and nitrosative stresses and persist in macrophages successfully, M. tuberculosis is endowed with a very efficient antioxidant complex.
Hu, Y, Yanmin Hu, Anthony R M Coates, Anthony R. M. Coates, Coates, AR   +4 more
core   +2 more sources

Untersuchung von Anti‐Glykan‐Antikörpern und Erforschung ihres medizinischen Potenzials mithilfe synthetischer Glykane

Angewandte Chemie, EarlyView.
Anti‐Glykan‐Antikörper spielen sowohl bei Gesundheit als auch bei Krankheit eine wesentliche Rolle. Der Zugang zu neuartigen Glykanstrukturen ermöglicht Fortschritte bei der Antikörpercharakterisierung für die Entdeckung von Biomarkern, die Antikörperentwicklung und das Impfstoffdesign.
Fabienne Weber, Aina Valenti, Jiří Ledvinka, Oren Moscovitz, Peter H. Seeberger   +4 more
wiley   +1 more source