Results 211 to 220 of about 62,427 (259)

Distribution of phenotypically disparate myocyte subpopulations in airway smooth muscle

Canadian Journal of Physiology and Pharmacology, 2005
Phenotype and functional heterogeneity of airway smooth muscle (ASM) cells in vitro is well known, but there is limited understanding of these features in vivo. We tested whether ASM is composed of myocyte subsets differing in contractile phenotype marker expression. We used flow cytometry to compare smooth muscle myosin heavy chain (smMHC) and smooth
Halayko, AJ, Stelmack, GL, Yamasaki, A, McNeill, K, Unruh, H, Rector, E   +5 more
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The contractile strength of vascular smooth muscle myocytes is shape dependent

Integrative Biology, 2014
Vascular smooth muscle cells in muscular arteries are more elongated than those in elastic arteries. Previously, we reported changes in the contractility of engineered vascular smooth muscle tissue that appeared to be correlated with the shape of the constituent cells, supporting the commonly held belief that elongated muscle geometry may allow for the
Ye G. J. C., Aratyn-Schaus Y., Nesmith A. P., Pasqualini F., Alford P. W., Parker K. K.   +5 more
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Adrenergic stimulation of diacylglycerol production in genital tract smooth muscle myocytes

In Vitro Cellular & Developmental Biology - Animal, 1993
Diacylglycerol (DAG) production has not been reported in previous studies that have characterized inositol phosphate production during alpha-1 adrenergic receptor signal transduction in the DDT1 MF-2 genital tract myocytes. The current study sought to measure norepinephrine (NE)-stimulated DAG production in these transformed myocytes utilizing thin ...
R J, Austin, L, DiGiovonni, M, Phillippe
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Use of cultured airway myocytes for study of airway smooth muscle

American Journal of Physiology-Lung Cellular and Molecular Physiology, 1995
Cultured airway smooth muscle cells provide a convenient model system for studying the regulation of a wide range of airway responses at the cellular level. This review describes the characteristics of cultured airway smooth muscle cells and differences that exist between cultured cells and acutely dissociated cells or muscle strips.
I P, Hall, M, Kotlikoff
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Three-Dimensional Culture of Human Uterine Smooth Muscle Myocytes on a Resorbable Scaffolding

Tissue Engineering, 2003
The objective of this study was to develop a three-dimensional culture system for the study of human myometrial physiology. Primary cell lines were initiated from human myometrium obtained at the time of term cesarean delivery. After several passages, cells were seeded onto a polyglactin-910 (Vicryl) mesh and maintained in culture.
Roger C, Young, Ralph, Schumann, Peisheng, Zhang   +2 more
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Altered Ion Channel Activity in Murine Colonic Smooth Muscle Myocytes in an Experimental Colitis Model

Biochemical and Biophysical Research Communications, 2000
We have investigated the activity of calcium and potassium channels in a murine model of experimental colitis. Colonic myocytes from dextran sulphate sodium (DSS)-treated mice were examined by whole cell patch clamp techniques. Myeloperoxidase activity was enhanced 3. 5-fold in DSS-treated mouse colon.
AKBARALI HI, POTHOULAKIS C, CASTAGLIUOLO, IGNAZIO   +2 more
openaire   +3 more sources

Responses of subcultured rat aortic smooth muscle myocytes to vasoactive agents and KCl-induced depolarization

American Journal of Physiology-Cell Physiology, 1991
We have developed a culture system in which a single-mass primary culture can be used for as long as 6 wk as a source of subcultured smooth muscle myocytes for the study of the changes of their shape upon addition of vasoactive agents (angiotensin, vasopressin, norepinephrine, and serotonin) and KCl depolarization.
P, Bodin, S, Richard, C, Travo, P, Berta, J C, Stoclet, S, Papin, P, Travo   +6 more
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Vesnarinone Inhibits Adenosine Uptake in Endothelial Cells, Smooth Muscle Cells and Myocytes, and Mediates Cytoprotection

Journal of Molecular and Cellular Cardiology, 1997
Vesnarinone is a novel synthetic inotropic agent. Recently, it has been reported that vesnarinone inhibits adenosine uptake in the B-lymphocytoid cell line. Since extracellular adenosine is cardioprotective, we examined whether vesnarinone inhibits adenosine uptake in cells constituting the cardiovascular system. 1 microCi of -3H-adenosine was added to
M, Kitakaze, M, Fong, M, Yoshitake, T, Minamino, K, Node, Y, Okuyama, N, Terada, T, Kambayashi, M, Hori   +8 more
openaire   +2 more sources

Myocyte Enhancer Binding Factor-2 Expression and Activity in Vascular Smooth Muscle Cells

Circulation Research, 1996
Abstract Proliferation and phenotypic modulation of smooth muscle cells (SMCs) are major components of the vessel’s response to injury in experimental models of restenosis. Some of the growth factors involved in restenosis have been identified, but to date little is known about the transcription factors that ultimately regulate
A B, Firulli, J M, Miano, W, Bi, A D, Johnson, W, Casscells, E N, Olson, J J, Schwarz   +6 more
openaire   +2 more sources

High-efficiency somatic mutagenesis in smooth muscle cells and cardiac myocytes in SM22?-Cre transgenic mice

genesis, 2005
The cytoskeletal protein SM22alpha is expressed in visceral and vascular smooth muscle cells (SMCs), in cardiac myocytes, and in the myotomal components of the somites during murine embryonic development. In this report, we describe the generation and characterization of transgenic mice expressing Cre-recombinase under the transcriptional control of ...
John J, Lepore, Lan, Cheng, Min, Min Lu, Patricia A, Mericko, Edward E, Morrisey, Michael S, Parmacek   +5 more
openaire   +2 more sources