Results 131 to 140 of about 181,791 (317)
The relay-converter interface influences hydrolysis of ATP by skeletal muscle myosin II [PDF]
, 2015 The interface between relay and converter domain of muscle myosin is critical for optimal myosin performance. Using Drosophila melanogaster indirect flight muscle S1 we performed a kinetic analysis of the effect of mutations in the converter and relay ...
Geeves, M. +3 more
core
One must reconstitute the functions of interest from purified proteins
Frontiers in PhysiologyI am often asked by students and younger colleagues and now by the editors of this issue to tell the history of the development of the in vitro motility assay and the dual-beam single-molecule laser trap assay for myosin-driven actin filament movement ...
James A. Spudich
doaj +1 more source
Cytoskeleton, EarlyView.ABSTRACT Elastic tethers connect telomeres of separating chromosomes in anaphase of animal cells. Immunofluorescence staining of titin in crane‐fly spermatocytes, using 4 different antibodies, shows that the giant elastic protein titin seems to be a component of mitotic tethers: titin “strands” extend between separating chromosomes, connecting their ...
Demetra Economopoulos +5 more
wiley +1 more source
Molecular mechanisms of the regulation of ATPase cycle in striated muscle
, 2007 New data on the molecular mechanism of the regulation of ATPase cycle by troponin-tropomyosin system have been obtained in reconstructed muscle fibers by using the polarized fluorescence technique, which allowed us following the azimuthal movements of ...
Yuri S. Borovikov +2 more
core
, 2010 Phosphorylation of myosin by myosin light chain kinase (MLCK) is essential for smooth muscle contraction. In this study we show that caldesmon (CaD) is also phosphorylated in vitro by MLCK.
Bettina Sarg +7 more
core +1 more source
Pathophysiology of human hearing loss associated with variants in myosins
Frontiers in PhysiologyDeleterious variants of more than one hundred genes are associated with hearing loss including MYO3A, MYO6, MYO7A and MYO15A and two conventional myosins MYH9 and MYH14. Variants of MYO7A also manifest as Usher syndrome associated with dysfunction of the
Takushi Miyoshi +4 more
doaj +1 more source
Isoform Specificity of a Compound Targeting Actin Filaments Containing Tropomyosin Tpm1.8/1.9
Cytoskeleton, EarlyView.ABSTRACT The unbranched actin filaments in mammalian cells are usually composed of co‐polymers of a specific tropomyosin isoform with actin. Genetic manipulation has revealed that the tropomyosins largely define the functional properties of actin filaments in an isoform‐specific, non‐redundant manner.
Jeff Hook +2 more
wiley +1 more source
Effect of Catechins on the Physicochemical, Structural and Gel Properties of Myosin in Tilapia
Shipin gongye ke-jiThis study investigated the influence of different mass concentrations of catechin (0~1.00 mg/mL) on the physicochemical characteristics, structures, and gel properties of myosin, through analyses of the turbidity, particle size, Zeta potential, surface ...
Yuyu HANG, Yang ZHANG, Du TAN, Yaqin HU
doaj +1 more source
Cytoskeleton, EarlyView.ABSTRACT Cooperativity between cytoskeletal proteins is crucial for spatiotemporal coordination in biological processes, like oogenesis. In mammalian and Drosophila oogenesis, proper assembly and function of actin networks require coordination between actin assembly factors Spire and formins, as well as actin‐associated proteins like myosins and Rab ...
Joseph Y. Ong +7 more
wiley +1 more source
Early changes in rat diaphragm biology with mechanical ventilation
, 2003 To better characterize the effects of 24-hour mechanical ventilation on diaphragm, the expression of myogenic transcription factors, myosin heavy chains, and sarcoplasmic/endoplasmic reticulum calcium-ATPase pumps was examined in rats.
Decramer Marc +15 more
core +1 more source