Myosin Drives Retrograde F-Actin Flow in Neuronal Growth Cones [PDF]
, 1996 Actin filaments assembled at the leading edge of neuronal growth cones are centripetally transported via retrograde F-actin flow, a process fundamental to growth cone guidance and other forms of directed cell motility.
Espreafico, E.M +3 more
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Gelation properties of porcine muscle proteins [PDF]
, 1989 The effects of linearly increasing heating rates (17, 38 and 85°C/hr) and protein concentration (10, 20, 30, 40 and 50 mg/mL) on thermally induced gels made of extracted salt-soluble proteins were evaluated. Gel strength analyses indicated that the force
Camou Arriola, Juan Pedro
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Real-Time Measurement of Myosin–Nucleotide Noncovalent Complexes by Electrospray Ionization Mass Spectrometry [PDF]
, 2007 Nanoelectrospray ionization mass spectrometry has been used to measure the binding of ATP and ADP to the active site of rabbit skeletal myosin-S1. Increases in the molecular mass of myosin-S1 of 425±10Da were obtained with the binding of ADP to the ...
Ashcroft, Alison E., White, Howard D.
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Role of Actin DNase-I-Binding Loop in Myosin Subfragment 1-Induced Polymerization of G-actin: Implications for the Mechanism of Polymerization [PDF]
, 2005 Proteolytic cleavage of actin between Gly42 and Val43 within its DNase-I-binding loop (D-loop) abolishes the ability of Ca-G-actin to spontaneously polymerize in the presence of KCl.
Galińska-Rakoczy, Agnieszka +3 more
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Stochastic dynamics and mechanosensitivity of myosin II minifilaments [PDF]
Philipp J Albert et al 2014 New J. Phys. 16 093019, 2014 Tissue cells are in a state of permanent mechanical tension that is maintained mainly by myosin II minifilaments, which are bipolar assemblies of tens of myosin II molecular motors contracting actin networks and bundles. Here we introduce a stochastic model for myosin II minifilaments as two small myosin II motor ensembles engaging in a stochastic tug ...
arxiv +1 more source
Actin reorganization throughout the cell cycle mediated by motor proteins [PDF]
, 2021 Cortical actin networks are highly dynamic and play critical roles in shaping the mechanical properties of cells. The actin cytoskeleton undergoes significant reorganization over the course of the cell cycle, when cortical actin transitions between open patched meshworks, homogeneous distributions, and aligned bundles.
arxiv +1 more source
Fluorescent probes of the orientation of myosin regulatory light chains in relaxed, rigor, and contracting muscle. [PDF]
, 1996 The orientation of the light-chain region of myosin heads in relaxed, rigor, and isometrically contracting fibers from rabbit psoas muscle was studied by fluorescence polarization. Cysteine 108 of chicken gizzard myosin regulatory light chain (cgRLC) was
Irving, M. +4 more
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Proteolysis and the domain organization of myosin subfragment 1. [PDF]
Proceedings of the National Academy of Sciences, 1984 Because the proteolytic cleavage of a folded polypeptide depends not only on the specificity of the protease but on the nature of the folding, we investigated the cleavage of (chymotryptically produced) subfragment 1 (designated "S-1") or "head" segment of myosin by seven proteases with different specificities. All seven produced approximately the same
Dominique Mornet +2 more
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Sensitivity of small myosin II ensembles from different isoforms to mechanical load and ATP concentration [PDF]
Phys. Rev. E 94, 052403 (2016), 2016 Based on a detailed crossbridge model for individual myosin II motors, we systematically study the influence of mechanical load and adenosine triphosphate (ATP) concentration on small myosin II ensembles made from different isoforms. For skeletal and smooth muscle myosin II, which are often used in actomyosin gels that reconstitute cell contractility ...
arxiv +1 more source
On the mechanism of energy transduction in myosin subfragment 1. [PDF]
Proceedings of the National Academy of Sciences, 1984 It is proposed that the myosin subfragment 1 moiety of the muscle contractile apparatus is a self-contained "engine" whose operational plan is based on the interactive nature of ATP (or degradation intermediate) binding and actin binding, made possible by an intersite communication system.
P Torgerson +6 more
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