Results 31 to 40 of about 2,706 (221)

Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin, and heavy meromyosin. [PDF]

, 1991
The substrate specificities of dynein, kinesin, and myosin substrate turnover activity and cytoskeletal filament-driven translocation were examined using 15 ATP analogues.
Furusawa, K, Malik, F, Ohashi, S, Okuno, M, Shimizu, T, Toyoshima, YY, Vale, RD   +6 more
core   +2 more sources

Novel functions of peroxiredoxin Q from Deinococcus radiodurans R1 as a peroxidase and a molecular chaperone

FEBS Letters, Volume 593, Issue 2, Page 219-229, January 2019., 2019
Deinococcus radiodurans R1 is extremely resistant to ionizing radiation and oxidative stress. In this study, we characterized DR0846, a candidate peroxiredoxin in D. radiodurans. DR0846 is a peroxiredoxin Q containing two conserved cysteine residues. DR0846 exists mainly in monomeric form with an intramolecular disulfide bond between the two cysteine ...
Chuloh Cho, Gun Woong Lee, Sung H. Hong, Shubhpreet Kaur, Kwang‐Woo Jung, Jong‐Hyun Jung, Sangyong Lim, Byung Yeoup Chung, Seung Sik Lee   +8 more
wiley   +1 more source

A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence [PDF]

, 1977
Rabbit antibodies against actin and tubulin were used in an indirect immunofluorescence study of the structure of the mitotic spindle of PtK1 cells after lysis under conditions that preserve anaphase chromosome movement. During early prophase there is no
Cande, W. Zacheus, Lazarides, Elias, McIntosh, J. Richard   +2 more
core   +5 more sources

Crosslinked myosin subfragment 1: a stable analogue of the subfragment-1.ATP complex. [PDF]

Proceedings of the National Academy of Sciences, 1983
Myosin subfragment 1 (S-1) with its two reactive cysteine groups crosslinked by N,N'-p-phenylenedimaleimide (pPDM), is shown to be a stable analogue of S-1 X ATP and S-1 X ADP X Pi, the predominant complexes present during the steady-state hydrolysis of ATP by S-1.
Joseph M. Chalovich, Evan Eisenberg, Lois E. Greene   +2 more
openaire   +3 more sources

The central domain of UNC‐45 chaperone inhibits the myosin power stroke

FEBS Open Bio, Volume 8, Issue 1, Page 41-48, January 2018., 2018
The multidomain UNC‐45B chaperone is crucial for the proper folding and function of sarcomeric myosin. Here, we show—using in vitro myosin motility and ATPase assays—that the central domain of UNC‐45B inhibits myosin power stroke through a mechanism that allows ATP turnover. Based on these data, we propose a novel model for the action of this chaperone
Paul J. Bujalowski, Paul Nicholls, Eleno Garza, Andres F. Oberhauser   +3 more
wiley   +1 more source

Direct Regulation of Striated Muscle Myosins by Nitric Oxide and Endogenous Nitrosothiols [PDF]

, 2010
, both through activation of guanylyl cyclase and through modification of cysteines in proteins to yield S-nitrosothiols. While NO affects the contractile apparatus directly, the identities of the target myofibrillar proteins remain unknown.
A Gow, AJ Kanai, Alicia M. Evangelista, Allan Doctor, Ashley R. Filo, B Guo, Benjamin Gaston, CA Gruetter, CK Roberts, David R. Jones, DE Harris, DE Harris, DJ Pinsky, FH Andrade, G Cuda, G Marechal, J Peterson, J Rodriguez, JD Pardee, JH Snook, KT Shiverick, L Liu, L Nogueira, LL Pearce, M Brunori, MW Allersma, Nadzeya V. Marozkina, NK Malavia, PW Han, S Galler, S Takahashi, S Umar, SJ Kron, SM Marino, SR Jaffrey, SS Margossian, Sudha Agarwal, Vijay S. Rao, VS Rao, WH Guilford, William H. Guilford, WJ Perkins, WW Kielley, Y Harada   +43 more
core   +2 more sources

Axon Initial Segment Cytoskeleton: Architecture, Development, and Role in Neuron Polarity

Neural Plasticity, Volume 2016, Issue 1, 2016., 2016
The axon initial segment (AIS) is a specialized structure in neurons that resides in between axonal and somatodendritic domains. The localization of the AIS in neurons is ideal for its two major functions: it serves as the site of action potential firing and helps to maintain neuron polarity.
Steven L. Jones, Tatyana M. Svitkina, Long-Jun Wu   +2 more
wiley   +1 more source

Fluctuation analysis of mechanochemical coupling depending on the type of bio-molecular motor [PDF]

, 2008
Mechanochemical coupling was studied for two different types of myosin motors in cells: myosin V, which carries cargo over long distances by as a single molecule; and myosin II, which generates a contracting force in cooperation with other myosin II molecules.
arxiv   +1 more source

Poorly Understood Aspects of Striated Muscle Contraction

BioMed Research International, Volume 2015, Issue 1, 2015., 2015
Muscle contraction results from cyclic interactions between the contractile proteins myosin and actin, driven by the turnover of adenosine triphosphate (ATP). Despite intense studies, several molecular events in the contraction process are poorly understood, including the relationship between force‐generation and phosphate‐release in the ATP‐turnover ...
Alf Månsson, Dilson Rassier, Georgios Tsiavaliaris, Oleg S. Matusovsky   +3 more
wiley   +1 more source

Twirling of actin by myosins II and V observed via polarized TIRF in a modified gliding assay [PDF]

, 2008
The force generated between actin and myosin acts predominantly along the direction of the actin filament, resulting in relative sliding of the thick and thin filaments in muscle or transport of myosin cargos along actin tracks. Previous studies have also detected lateral forces or torques that are generated between actin and myosin, but the origin and
arxiv   +1 more source