Results 271 to 280 of about 10,628,077 (304)

N-Glycan processing and glycoprotein folding

2001
Publisher Summary This chapter describes how N-glycan processing participates in the folding and quality control mechanisms operating in the ER. Glycosylation of asparagine residues (N-glycosylation) in the endoplasmic reticulum (ER) presents two basic differences with respect to glycosylation of Ser/Thr residues (Ooglycosylation) in either the ...
E S, Trombetta, A J, Parodi
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Chemical and enzymatic N-glycan release comparison for N-glycan profiling of monoclonal antibodies expressed in plants

Analytical Biochemistry, 2010
Plants synthesize N-glycans containing the antigenic sugars alpha(1,3)-fucose and beta(1,2)-xylose. Therefore it is important to monitor these N-glycans in monoclonal antibodies produced in plants (plantibodies). We evaluated several techniques to characterize the N-glycosylation of a plantibody produced in tobacco plants with and without the KDEL ...
Triguero, Ada, Cabrera, Gleysin, Royle, Louise, Harvey, David, Rudd, Pauline, Dwek, Raymond, Bardor, Muriel, Lerouge, Patrice, Cremata, José   +8 more
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N-glycan production in the endoplasmic reticulum of plants

Trends in Plant Science, 2009
N-glycosylation is a complex process that encompasses the biosynthesis and modification of sugar moieties in the endoplasmic reticulum (ER) and Golgi. The ER-localized steps of N-glycan production in plants have received relatively little attention, despite their emerging roles in stress responses.
Richard J, Pattison, Anna, Amtmann
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Methods for the absolute quantification of N-glycan biomarkers

Biochimica et Biophysica Acta (BBA) - General Subjects, 2016
Many treatment options especially for cancer show a low efficacy for the majority of patients demanding improved biomarker panels for patient stratification. Changes in glycosylation are a hallmark of many cancers and inflammatory diseases and show great potential as clinical disease markers.
Juan, Etxebarria, Niels-Christian, Reichardt   +1 more
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Genetic model organisms in the study of N-glycans

Biochimie, 2001
Recently the genomic sequences of three multicellular eukaryotes, Caenorhabditis elegans, Drosophila melanogaster and Arabidopsis thaliana, have been elucidated. A number of cDNAs encoding glycosyltransferases demonstrated to have a role in N-linked glycosylation have already been cloned from these organisms, e.g., GlcNAc transferases and alpha 1,3 ...
F, Altmann, G, Fabini, H, Ahorn, I B, Wilson   +3 more
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N-glycan structures: recognition and processing in the ER

Trends in Biochemical Sciences, 2010
The processing of N-linked glycans determines secretory protein homeostasis in the eukaryotic cell. Folding and degradation of glycoproteins in the endoplasmic reticulum (ER) are regulated by molecular chaperones and enzymes recruited by specific oligosaccharide structures.
Aebi Markus, Bernasconi Riccardo, Clerc Simone, Molinari Maurizio   +3 more
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Synthesis of homogeneous glycoproteins with diverse N-glycans

2022
In the post-genomic era, post- and co-translational modifications (P/C-TM) of proteins are known as the more essential elements for the activation of protein function. Among these protein modifications, glycosylation is one of the most abundant modifications in eukaryotic cells. The synthesis of glycoproteins with uniform glycan structures is essential
Kota, Nomura, Yanbo, Liu, Yasuhiro, Kajihara   +2 more
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N-glycans in Toxicodendron vernicifluum lacquer laccase

Carbohydrate Research, 2019
The N-glycans in Toxicodendron vernicifluum (Rhus vernicifera) lacquer laccase was elucidated for the first time through a combination of enzymatic digestion and subsequent mass spectrometry measurements using LC-MS/MS and MALDI-TOF MS. Lacquer laccase was isolated from a Japanese lacquer acetone powder from consecutive Sephadex C-50 and DEAE A-50 ...
Oyunjargal Tumurbaatar, Surina Bo, Oyundelger Ganzorig, Kensuke Miyazaki, Takashi Yoshida   +4 more
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N-glycan terocessing in the ER

1994
Abstract N-linked oligosaccharide synthesis begins with the transfer of a precursor oligosaccharide from a dolichol-P-P-linked intermediate to Asn-X-Ser/Thr. This oligosaccharide, Glc3Man9GlcNAc2, in most cells is then trimmed by a-glucosidases and a,-mannosidases, beginning in the ER.
R Trimbe, K Morcrnen,, A Herscovics
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Role of N-glycans in growth factor signaling

Glycoconjugate Journal, 2003
Secreted proteins and membrane proteins are frequently post-translationally modified by oligosaccharides. Therefore, many glycoproteins are involved in signal transduction. One example is growth factor receptors, which are membrane proteins that often contain oligosaccharides.
Motoko, Takahashi, Takeo, Tsuda, Yoshitaka, Ikeda, Koichi, Honke, Naoyuki, Taniguchi   +4 more
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