Results 31 to 40 of about 3,459 (156)

ADDITIONAL CHARACTERIZATION OF ADENOSINE NUCLEOSIDASE FROM ALASKA PEA SEEDS [PDF]

, 2017
Adenosine nucleosidase was purified from Alaska pea seeds six days after germination. A 3-fold purification has been reached with a 0.56 % recovery. The purification scheme involved ammonium sulfate precipitation between 30% and 60% saturation, followed ...
Alruwaili, Awatif Hanif
core   +7 more sources

N‐ to C‐Glycoside Rearrangement of Uridine 5′‐Phosphate in Two Enzymatic Steps for the Production of Pseudouridine 5′‐Phosphate

Biotechnology and Bioengineering, Volume 122, Issue 9, Page 2456-2464, September 2025.
The authors present a streamlined cascade reaction for one‐pot N‐C rearrangement of uridine‐5′‐phosphate (UMP) to pseudouridine‐5′‐phosphate (ΨMP; a key mRNA vaccine building block), in two enzymatic steps catalyzed by UMP nucleosidase (EC 3.2.2.10) and ΨMP C‐glycosidase (EC 4.2.1.70).
Martin Pfeiffer, Franziska Guld, Bernd Nidetzky   +2 more
wiley   +1 more source

Shoot Nitrate Status Regulates Arabidopsis Shoot Growth and Systemic Transcriptional Responses via Shoot Adenosine Phosphate‐Isopentenyltransferase 3

Physiologia Plantarum, Volume 177, Issue 5, September/October 2025.
ABSTRACT Plants systemically regulate growth and gene expression according to their internal nitrate status. Our previous study reported that shoot nitrate accumulation increases shoot expression of adenosine phosphate‐ISOPENTENYLTRANSFERASE 3 (IPT3) and shoot levels of N6‐(Δ2‐isopentenyl) adenine (iP)‐type cytokinins (CKs).
Kota Monden, Takamasa Suzuki, Mikiko Kojima, Yumiko Takebayashi, Daisuke Sugiura, Tsuyoshi Nakagawa, Hitoshi Sakakibara, Takushi Hachiya   +7 more
wiley   +1 more source

Structural and Biochemical Characterization of Chlamydia trachomatis Hypothetical Protein CT263 Supports That Menaquinone Synthesis Occurs through the Futalosine Pathway [PDF]

, 2014
BACKGROUND: Specific pathways and components for respiration in Chlamydia are poorly understood. RESULTS: The C. trachomatis hypothetical protein CT263 crystal structure displays strong structural similarity with 5′-methylthioadenosine nucleosidase ...
Barta, Michael L., Battaile, Kevin P., Hefty, P. Scott, Lovell, Scott, Schramm, Vern L., Thomas, Keisha, Yuan, Hongling   +6 more
core   +1 more source

Co‐Expression of MHC‐II and ANXA1: Mediators of PD‐1/PD‐L1 Therapy Resistance in Breast Cancer

Cancer Reports, Volume 8, Issue 8, August 2025.
ABSTRACT Background Triple‐negative breast cancer (TNBC) presents significant treatment challenges and poor prognosis. While immune checkpoint blockade (ICB) therapy shows promise, patient responses vary widely, highlighting the urgent need for reliable biomarkers to predict efficacy and guide treatment decisions.
Hao Wang, Ji‐Feng Sun, Chen Wang, Zhan‐Sheng Jiang, Zhong‐Sheng Tong   +4 more
wiley   +1 more source

Modeling and Optimization of Microwave Vacuum Drying for Pinelliae Rhizoma: Integrating Drying Kinetics, Artificial Neural Networks, and Quality Preservation

Food Science &Nutrition, Volume 13, Issue 8, August 2025.
The peeled Pinellia ternata (Thunb.) Breit tubers were dried with the microwave vacuum drying oven. Then, its drying characteristics, contents of nucleosides, and surface microstructural alterations of dried samples were determined, respectively. Drying parameters were predicted and optimized using four different mathematical models and artificial ...
Pan Wang, Xiaoping Yang, Xiaopeng Huang, Guojun Ma, Shidong Zhang, Siqi Wang, Ming Wang, Zewen Zhu, Yanrui Xu, ZePeng Zang, Xu Liu   +10 more
wiley   +1 more source

Utilization of tryptophan, nicotinamide and nicotinic acid as precursors for nicotinamide nucleotide synthesis in isolated rat liver cells [PDF]

, 2005
1. Incubation of isolated rat hepatocytes with nicotinamide or nicotinic acid showed that while both vitamers were taken up from the incubation medium, neither was utilized to any significant extent as a precursor of the nicotinamide nucleotide coenzymes,
Bender, DA, Olufunwa, R
core   +1 more source

Disruption of mc1r Disturbs Skin Pigmentation in Xenopus tropicalis

Pigment Cell &Melanoma Research, Volume 38, Issue 4, July 2025.
ABSTRACT The melanocortin 1 receptor (MC1R) is well‐established as a pivotal regulator of pigmentation in various species. Despite a wealth of research focused on mammals and fish, the role of Mc1r in amphibians has remained largely unexplored. This study was designed to elucidate the contribution of Mc1r in Xenopus tropicalis.
Lanxin Li, Jixuan Huang, Yonglong Chen, Rensen Ran   +3 more
wiley   +1 more source

In Vitro Psilocybin Synthesis by Co‐Immobilized Enzymes

Chemistry – A European Journal, Volume 31, Issue 29, May 22, 2025.
The drug candidate and psychedelic mushroom product psilocybin was produced by multienzyme‐charged beads in a biocatalytic cell‐free approach. Using immobilized fungal indolethylamine biosynthetic and additional bacterial enzymes, these beads turned over 4‐hydroxy‐l‐tryptophan to psilocybin.
Tim Schäfer, Alexander Sherwood, Thomas Kirkland, Thomas Krüger, Jakob Worbs, Olaf Kniemeyer, Markus Gressler, Dirk Hoffmeister   +7 more
wiley   +1 more source

Reconstitution of Methionine Cycle With ATP Regeneration for Whole‐Cell Catalysis of Creatine Production in Engineered Escherichia coli

Microbial Biotechnology, Volume 18, Issue 5, May 2025.
In this study, we constructed a CR biosynthesis pathway in E. coli by introducing exogenous AGAT and GAMT. We integrated the reconstitution methionine cycle with a polyphosphate kinase‐based ATP regeneration system, achieving a CR titre of 5.27 g/L with a productivity of 0.22 g/L/h.
Yuhua Sheng, Yaokang Wu, Linpei Zhang, Xueqin Lv, Jianghua Li, Long Liu, Guocheng Du, Jian Chen, Yanfeng Liu   +8 more
wiley   +1 more source