NADP(H)-dependent biocatalysis without adding NADP(H)
Proceedings of the National Academy of Sciences, 2022 Isocitrate dehydrogenase 1 (IDH1) naturally copurifies and crystallizes in a resting state with a molecule of its exchangeable cofactor, NADP + /NADPH, bound in each monomer of the homodimer. We report electrochemical studies with IDH1 that exploit this property to reveal the massive advantage of nanoconfinement to ...
Herold, RA +3 more
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Preferential utilization of NADPH as the endogenous electron donor for NAD(P)H:quinone oxidoreductase 1 (NQO1) in intact pulmonary arterial endothelial cells [PDF]
, 2009 The goal was to determine whether endogenous cytosolic NAD(P)H:quinone oxidoreductase 1 (NQO1) preferentially uses NADPH or NADH in intact pulmonary arterial endothelial cells in culture.
Bongard, Robert D +3 more
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Whole blood NAD and NADP concentrations are not depressed in subjects with clinical pellagra [PDF]
, 2007 Population surveys for niacin deficiency are normally based on clinical signs or on biochemical measurements of urinary niacin metabolites. Status may also be determined by measurement of whole blood NAD and NADP concentrations. To compare these methods,
Cheung, E +5 more
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Cofactor regeneration by a soluble pyridine nucleotide transhydrogenase for biological production of hydromorphone [PDF]
, 2000 We have applied the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens to a cell-free system for the regeneration of the nicotinamide cofactors NAD and NADP in the biological production of the important semisynthetic opiate drug ...
Boonstra, B +4 more
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Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development [PDF]
, 2011 <p>Background: Plasmodium falciparum contains three genes encoding potential glutamate dehydrogenases. The protein encoded by gdha has previously been biochemically and structurally characterized.
Aparicio, I. +7 more
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The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves [PDF]
, 2009 The non-photosynthetic NADP-malic enzyme EC 1.1.1.40 (NADP-ME), which catalyzes the oxidative decarboxylation of L-malate and NADP+ to produce pyruvate and NADPH, respectively, and which could be involved in plant defense responses, was isolated from ...
HELENA RYŠLAVÁ +3 more
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Structural studies of glucose-6-phosphate and NADP+ binding to human glucose-6-phosphate dehydrogenase [PDF]
, 2005 Human glucose-6-phosphate dehydrogenase (G6PD) is NADP(+)-dependent and catalyses the first and rate-limiting step of the pentose phosphate shunt. Binary complexes of the human deletion mutant, DeltaG6PD, with glucose-6-phosphate and NADP(+) have been ...
Adams, MJ +5 more
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NADP(H) allosterically regulates the interaction between ferredoxin and ferredoxin‐NADP+ reductase [PDF]
FEBS Open Bio, 2019 Ferredoxin‐NADP+ reductase (FNR) in plants receives electrons from ferredoxin (Fd) at the end of the photosynthetic electron transfer chain and converts NADP+ to NADPH. The interaction between Fd and FNR in plants was previously shown to be attenuated by NADP(H).
Yoko Kimata‐Ariga +6 more
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NADP(+)-isocitrate dehydrogenase from the cyanobacterium Anabaena sp. strain PCC 7120: purification and characterization of the enzyme and cloning, sequencing, and disruption of the icd gene [PDF]
, 1994 NADP(+)-isocitrate dehydrogenase (NADP(+)-IDH) from the dinitrogen-fixing filamentous cyanobacterium Anabaena sp. strain PCC 7120 was purified to homogeneity.
Florencio Bellido, Francisco Javier +1 more
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Divergent evolution of protein conformational dynamics in dihydrofolate reductase. [PDF]
, 2013 Molecular evolution is driven by mutations, which may affect the fitness of an organism and are then subject to natural selection or genetic drift. Analysis of primary protein sequences and tertiary structures has yielded valuable insights into the ...
Bhabha, Gira +9 more
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