Results 71 to 80 of about 832 (217)

Synthese und aktivitätsbasierte Proteinprofilierung identifizieren die Aldo‐Keto‐Reduktase 1C3 als Zielprotein der Myxoglucamide

Angewandte Chemie, EarlyView.
Myxoglucamid A – ein neuartiges, aus Myxobakterien isoliertes Glykolipopeptid – wurde erstmals totalsynthetisch hergestellt. Mittels aktivitätsbasierter Proteinprofilierung (ABPP) wurde die Aldo‐Keto‐Reduktase 1C3 (AKR1C3) als primäres Zielprotein in menschlichen Zellen identifiziert.
Thomas Siemon, Vivek K. Mishra, Mingming Zhao, Konrad Büssow, Alexander Popoff, Chengzhang Fu, Rolf Müller, Lothar Jänsch, Wulf Blankenfeldt, Mark Brönstrup   +9 more
wiley   +1 more source

Dihydropyrimidine dehydrogenase. Kinetic mechanism for reduction of uracil by NADPH.

Journal of Biological Chemistry, 1993
Steady-state and pre-steady-state kinetic data were used to determine the kinetic mechanism for bovine liver dihydropyrimidine dehydrogenase (DPDase). Steady-state kinetic data suggested a random rapid-equilibrium mechanism with Km values for NADPH and uracil of 0.12 microM and 0.8 microM, respectively, and a kcat of 1.6 s-1 in Tris buffer at pH 8.0 ...
D J, Porter, T, Spector
openaire   +2 more sources

Synthesis and Activity‐Based Protein Profiling Identifies Aldo‐Keto Reductase 1C3 as Target Protein of Myxoglucamides

Angewandte Chemie International Edition, EarlyView.
Myxoglucamide A—a novel glycolipopeptide isolated from myxobacteria—was synthesized, and activity‐based protein profiling identified aldo‐keto reductase 1C3 (AKR1C3) as its main target in human cells. Structure‐activity relationship studies, together with biophysical characterization and X‐ray crystallography, elucidated the structural basis of AKR1C3 ...
Thomas Siemon, Vivek K. Mishra, Mingming Zhao, Konrad Büssow, Alexander Popoff, Chengzhang Fu, Rolf Müller, Lothar Jänsch, Wulf Blankenfeldt, Mark Brönstrup   +9 more
wiley   +1 more source

NADPH‐related processes studied with a SoxR‐based biosensor in Escherichia coli

MicrobiologyOpen, 2019
NADPH plays a crucial role in cellular metabolism for biosynthesis and oxidative stress responses. We previously developed the genetically encoded NADPH biosensor pSenSox based on the transcriptional regulator SoxR of Escherichia coli, its target ...
Alina Spielmann, Meike Baumgart, Michael Bott   +2 more
doaj   +1 more source

Dehalogenating and NADPH-modifying activities of dihydropyrimidine dehydrogenase.

Journal of Biological Chemistry, 1994
Dihydropyrimidine dehydrogenase (DPDase) catalyzed the debromination of 5-bromo-5,6-dihydrouracil (BrUH2) to uracil at pH 7.7 and 37 degrees C. The debrominating activity of DPDase was increased 5-fold by treatment with H2O2, whereas the dehydrogenating activity was inhibited by this treatment.
openaire   +2 more sources

Quantum‐Level Influences on Enzyme Reactions and Their Ripple Effects on Biological Phenomena

Advanced Physics Research, EarlyView.
Because living organisms are ultimately composed of atoms and molecules, quantum‐mechanical effects are expected to contribute to biological phenomena. Quantum biology seeks to elucidate life processes from a quantum‐mechanical perspective. This review discusses how quantum‐level effects, including quantum tunneling, may propagate from enzymatic proton‐
Takeshi Yasuda
wiley   +1 more source

Consumption of NADPH for 2-HG Synthesis Increases Pentose Phosphate Pathway Flux and Sensitizes Cells to Oxidative Stress

Cell Reports, 2018
Summary: Gain-of-function mutations in isocitrate dehydrogenase 1 (IDH1) occur in multiple types of human cancer. Here, we show that these mutations significantly disrupt NADPH homeostasis by consuming NADPH for 2-hydroxyglutarate (2-HG) synthesis. Cells
Susan J. Gelman, Fuad Naser, Nathaniel G. Mahieu, Lisa D. McKenzie, Gavin P. Dunn, Milan G. Chheda, Gary J. Patti   +6 more
doaj   +1 more source

TWEAK/Fn14 Signaling Drives Oxidative Cardiac Injury in Systemic Lupus Erythematosus: Evidence From Patient Biomarker Studies, Lupus Mouse Models, and Cardiomyocyte Assays

Arthritis &Rheumatology, EarlyView.
Objective Cardiac involvement is a major cause of morbidity in systemic lupus erythematosus (SLE). Tumor necrosis factor–like weak inducer of apoptosis (TWEAK) is elevated in SLE, but its contribution to lupus‐associated cardiac injury is unclear. We investigated the role of TWEAK/fibroblast growth factor–inducible 14 (Fn14) signaling in SLE‐related ...
Yale Liu, Zhu Yan, Xueting Peng, Meixuan Li, Juan Wang, Yan Zhang, Xiaoqian Hu, Mingzhu Zhou, Kaixuan Ren, Dan Zhang, Xingyi Guo, Yumin Xia, Huanhuan Huo   +12 more
wiley   +1 more source

Spray‐Dried Engineered Escherichia coli Whole‐Cell Biocatalysts Enable Cell Recycling and In Vivo α‐Ketoglutarate and NADPH Regeneration for Efficient Codeine Manufacture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A recombinant whole‐cell biocatalytic approach provides several advantages over isolated enzymes, such as improved enzyme stability, in vivo cofactor regeneration, and the facilitation of cascade reactions within a single cell. Whole‐cell biocatalysis has gained attention in medicinal opioid production, due to its technical and economic ...
Ali Jahanian, Carlos Horacio Luna‐Flores, Xu Li, Sally L. Gras, Robert E. Speight   +4 more
wiley   +1 more source

Artificial Cell Catalysts for Biotransformation: Design Principles and Process Integration

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Artificial cell catalysts are emerging as programmable biocatalytic platforms for sustainable molecular conversion. By reconstituting enzymes and functional modules within synthetic compartments, they combine the selectivity of biological catalysis with the design flexibility of synthetic materials.
Iori Kobayashi, Shogo Yoshimoto, Katsutoshi Hori   +2 more
wiley   +1 more source