Results 11 to 20 of about 1,581 (100)

Differential effects of lipid composition on the thermal and functional properties of membrane associated CYP2J2. [PDF]

Protein Sci
Abstract CYP2J2 is a membrane‐bound cytochrome P450 that is expressed in cardiomyocytes, where it is known to metabolize arachidonic acid into cardioprotective epoxyeicosatrienoic acids (EETs). It consists of transmembrane domains embedded in the hydrophobic segments of the cell membrane and surrounded by various lipids.
Das R, Mastrion HM, Vassar HB, Das A.
europepmc   +2 more sources

Reductive Methylation: An Alternative to Lysine → Arginine Mutagenesis. [PDF]

J Pept Sci
Replacing lysine with arginine, the standard genetic approach to eliminate amine reactivity, alters physicochemical properties and leaves the N terminus unprotected. Here, reductive methylation is characterized as a conservative chemical alternative. Complete dimethylation of human ribonucleases preserves thermostability, protein–protein interaction ...
Molina OJ, Gutierrez CS, Yang J, Wralstad EC, Raines RT.   +4 more
europepmc   +2 more sources

Oxidative Implications of Substituting a Conserved Cysteine Residue in Sugar Beet Phytoglobin BvPgb 1.2

Antioxidants, 2022
Phytoglobins (Pgbs) are plant-originating heme proteins of the globin superfamily with varying degrees of hexacoordination. Pgbs have a conserved cysteine residue, the role of which is poorly understood.
Simon Christensen, Leonard Groth, Nélida Leiva-Eriksson, Maria Nyblom, Leif Bülow   +4 more
doaj   +1 more source

Caprin-1 binding to the critical stress granule protein G3BP1 is influenced by pH

Open Biology, 2023
G3BP is the central node within stress-induced protein–RNA interaction networks known as stress granules (SGs). The SG-associated proteins Caprin-1 and USP10 bind mutually exclusively to the NTF2 domain of G3BP1, promoting and inhibiting SG formation ...
Tim Schulte, Marc D. Panas, Xiao Han, Lucy Williams, Nancy Kedersha, Jonas Simon Fleck, Timothy J. C. Tan, Xaquin Castro Dopico, Anders Olsson, Ainhoa Moliner Morro, Leo Hanke, Johan Nilvebrant, Kim Anh Giang, Per-Åke Nygren, Paul Anderson, Adnane Achour, Gerald M. McInerney   +16 more
doaj   +1 more source

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project [PDF]

Journal of Visualized Experiments, 2019
The Horizon2020 Virus-X project was established in 2015 to explore the virosphere of selected extreme biotopes and discover novel viral proteins. To evaluate the potential biotechnical value of these proteins, the analysis of protein structures and functions is a central challenge in this program. The stability of protein sample is essential to provide
Bruce, D, Cardew, E, Freitag-Pohl, S, Pohl, E   +3 more
openaire   +4 more sources

ATP modulates holdase activity of fungal and human 110-kilodalton heat shock proteins to promote protein folding. [PDF]

Protein Sci
Abstract Heat shock proteins of the 70‐kDa family (Hsp70s) are highly abundant and conserved molecular chaperones that help preserve proteostasis primarily by facilitating proper protein folding. Heat shock protein of the 110‐kDa family (Hsp110s), a specialized branch of the Hsp70/Hsp110 superfamily, function both as nucleotide exchange factor (NEF ...
Kidd JM, Sun C, Garay A, Keplinger M, Richter C, May AE, Liu Q.   +6 more
europepmc   +2 more sources

Monitoring of Metabolic Disorders Through Electro-Sensing of Riboflavin by Novel Super-Conductive Silver Ink Modified Paper. [PDF]

Anal Sci Adv
ABSTRACT Riboflavin (RIB), or vitamin B2, plays a key role in cellular protection against oxidative stress, and its deficiency can cause severe physiological complications, including metabolic disorders, photosensitivity, anaemia, growth delay and gastrointestinal problems.
Ebrahimi R, Hasanzadeh M, Shadjou N.
europepmc   +2 more sources

Comprehensive Biophysical Profiling Evidences Self-Oligomerization of Bacterially Expressed Pc Protein. [PDF]

Chembiochem
Polycomb (Pc), a core component of Polycomb Repressive Complex 1 (PRC1), is largely intrinsically disordered yet forms buffer‐ and concentration‐dependent higher‐order assemblies. Integrative biophysical analysis and cryo‐electron microscopy reveal dynamic, heterogeneous oligomers, whereas AlphaFold modeling suggests that structural organization is ...
Zahid M, Prajapati S, Alamdari G, Tittmann K, Kara S.   +4 more
europepmc   +2 more sources

SAXS and stability studies of iron-induced oligomers of bacterial frataxin CyaY. [PDF]

PLoS ONE, 2017
Frataxin is a highly conserved protein found in both prokaryotes and eukaryotes. It is involved in several central functions in cells, which include iron delivery to biochemical processes, such as heme synthesis, assembly of iron-sulfur clusters (ISC ...
Mostafa Fekry, Wessen Alshokry, Przemysław Grela, Marek Tchórzewski, Eva-Christina Ahlgren, Christopher A Söderberg, Oleksandr Gakh, Grazia Isaya, Salam Al-Karadaghi   +8 more
doaj   +1 more source

Synthesis and Activity‐Based Protein Profiling Identifies Aldo‐Keto Reductase 1C3 as Target Protein of Myxoglucamides

Angewandte Chemie International Edition, EarlyView.
Myxoglucamide A—a novel glycolipopeptide isolated from myxobacteria—was synthesized, and activity‐based protein profiling identified aldo‐keto reductase 1C3 (AKR1C3) as its main target in human cells. Structure‐activity relationship studies, together with biophysical characterization and X‐ray crystallography, elucidated the structural basis of AKR1C3 ...
Thomas Siemon, Vivek K. Mishra, Mingming Zhao, Konrad Büssow, Alexander Popoff, Chengzhang Fu, Rolf Müller, Lothar Jänsch, Wulf Blankenfeldt, Mark Brönstrup   +9 more
wiley   +1 more source