Results 81 to 90 of about 3,520 (152)

A Luciferase-Based Approach for Functional Screening of 5′ and 3′ Untranslated Regions of the mRNA Component for mRNA Vaccines

Vaccines
Background/Objectives: The recent COVID-19 pandemic caused by SARS-CoV-2 infection has highlighted the need for protocols for rapid development of efficient screening methods to search for the optimal mRNA vaccine structures against mutable viral agents.
Maria Rubtsova, Yuliana Mokrushina, Dmitry Andreev, Maria Poteshnova, Nikita Shepelev, Mariya Koryagina, Ekaterina Moiseeva, Diana Malabuiok, Yury Prokopenko, Stanislav Terekhov, Aleksander Chernov, Elena Vodovozova, Ivan Smirnov, Olga Dontsova, Alexander Gabibov, Yury Rubtsov   +15 more
doaj   +1 more source

Investigating Nanoluciferase as a Potential Proteome Stress Sensor

, 2016
Firefly luciferase (Fluc) is a well-documented proteome stress sensor. Its misfolding upon cellular stress leads to aggregation and loss of enzymatic activity, which can be assayed by oxidizing luciferin, producing bioluminescence. However, Fluc is large
Dong, Heidi, Xu, Sarah
core  

A human H5N1 influenza virus expressing bioluminescence for evaluating viral infection and identifying therapeutic interventions

iScience
Summary: A multistate outbreak of highly pathogenic avian influenza virus (HPAIV) H5N1 in the United States dairy cattle was first reported on March 2024, followed by a zoonotic cattle-to-human virus transmission to a dairy farm worker in Texas.
Ramya S. Barre, Ruby A. Escobedo, Esteban M. Castro, Michal Gazi, Joshua D. Castro, Anastasija Cupic, Mahmoud Bayoumi, Nathaniel Jackson, Chengin Ye, Aitor Nogales, Roy N. Platt, Ricardo Carrion, Jr., Timothy J.C. Anderson, Adolfo García-Sastre, Ahmed Mostafa, Luis Martinez-Sobrido   +15 more
doaj   +1 more source

Poster Sessions

HemaSphere, Volume 10, Issue S1, June 2026.
wiley   +1 more source

ISEV2026 Abstract Book

Journal of Extracellular Vesicles, Volume 15, Issue S1, June 2026.
wiley   +1 more source

A nanoluciferase-encoded bacteriophage illuminates viral infection dynamics of Pseudomonas aeruginosa cells

International audienceAbstract Bacteriophages (phages) are increasingly considered for both treatment and early detection of bacterial pathogens given their specificity and rapid infection kinetics.
Zborowsky, Sophia, Tournebize, Régis, Balacheff, Quentin, Theodorou, Ioanna, Weitz, Joshua, S, Kane, Rokhaya, Debarbieux, Laurent, Delattre, Raphaëlle   +7 more
core   +1 more source

Use of Recombinant Chikungunya Virus expressing Nanoluciferase to Identify Chondrocytes as Target Cells in an Immunocompetent Mouse Model

International audienceChikungunya virus (CHIKV) induces predominantly symptomatic infections, marked by fever, myalgia, rash and polyarthralgia that can last for up to 3 years after infection.
Desprès, Philippe, Belarbi, Essia, Gessain, Antoine, Jeannin, Patricia, Geolier, Virginie, Hardy, David, Choumet, Valérie, Kümmerer, Beate, Roques, Pierre, Ceccaldi, Pierre-Emmanuel, Legros, Vincent   +10 more
core   +1 more source

Reporter assays for HPV16 E1/E1C and E2 mRNA splicing using nanoluciferase and secreted luciferase

We have generated two reporter plasmids that either monitor expression of human papillomavirus type 16 (HPV16) E2 mRNAs using splice site SA2709 or HPV16 E1C mRNAs utilizing splice site SA2582.
Hao, Chengyu, Kajitani, Naoko, Zhao, Yani, Jiang, Zixiao, Schwartz, Stefan, Jönsson, Johanna, Zheng, Yunji, Wu, Chengjun, Cui, Xiaoxu   +8 more
core   +1 more source

Nanoluciferase reporter preserves immunocompetent glioma model fidelity while facilitating longitudinal molecular imaging

Glioblastoma studies employ syngeneic orthotopic models to preserve tumor-immune interactions, but intracranial tumor burden is challenging to monitor longitudinally.
Rasha Msallam, Ann-Marie Chacko, Joanne L Ong, Carla Bianca L Victorio, Wisna Novera, Shantanu Gupta, Sven Petersen, Eng Eong Ooi, Arun Ganasarajah   +8 more
core   +1 more source

How to Perform a Tracer Displacement BRET Assay for the TRPML1 Ion Channel. [PDF]

Bio Protoc
Cunha MR, Catta-Preta CMC, Massirer KB, Couñago RM.   +3 more
europepmc   +1 more source