Results 61 to 70 of about 3,520 (152)

Nanobody-Nanoluciferase Fusion Protein-Enabled Immunoassay for Ochratoxin A in Coffee with Enhanced Specificity and Sensitivity

, 2022
Ochratoxin A (OTA), one of the best-known mycotoxins, causes problems concerning food safety with potential toxic effects in humans and animals. So, it is crucial to develop simple and sensitive methods for the detection of OTA.
Hongmei Cao, Long Wu, Liu, Zilong, Cao, Hongmei, Zilong Liu, Xing Liu, Chen, Qi, Yujing Liao, Bao, Kunlu, Liu, Xing, Kunlu Bao, Liao, Yujing, Wu, Long, Qi Chen   +13 more
core   +1 more source

A nano-luciferase expressing human coronavirus OC43 for countermeasure development

Virus Research
The genetic diversity of the coronavirus (CoV) family poses a significant challenge for drug discovery and development. Traditional antiviral drugs often target specific viral proteins from specific viruses which limits their use, especially against ...
Meghan V. Diefenbacher, Thomas J. Baric, David R. Martinez, Ralph S. Baric, Nicholas J. Catanzaro, Timothy P. Sheahan   +5 more
doaj   +1 more source

Revisiting Hydrazine Moiety as a Scaffold for the Synthesis of Potential Antiviral Pyrazole Ribonucleoside Analogues

ChemMedChem, Volume 21, Issue 11, 15 June 2026.
Three series of pyrazole ribonucleoside analogues were designed and synthesized. They were evaluated, along with some of their pronucleotides, for their antiviral activity against RNA viruses. Pyrazole ribonucleoside analogues were prepared via a linear approach starting with the reaction of D‐ribosylhydrazine with 1,3‐dicarbonyl or acrylonitrile ...
Anaïs Guillou, Nathalie Gros, Delphine Muriaux, Suzanne Peyrottes, Jean‐Jacques Vasseur, Michael Smietana, Christophe Mathé   +6 more
wiley   +1 more source

One-Step Ultrasensitive Bioluminescent Enzyme Immunoassay Based on Nanobody/Nanoluciferase Fusion for Detection of Aflatoxin B₁ in Cereal

, 2019
Nanoluciferase (Nluc), the smallest luciferase known, was used as the fusion partner with a nanobody against aflatoxin B1 to develop a bioluminescent enzyme immunoassay (BLEIA) for detection of the aflatoxin B1 in cereal.
Bogdan Barnych (1548784), Jinheng Fu, Debin Wan (1996066), Qinghua He (214040), Zhui Tu (760330), Jinheng Fu (6629996), Yanping Li (90163), Wenjie Ren (3715270), Bruce D. Hammock (65754), Yang Xu (178421), Yang Xu, Bruce D. Hammock, Bogdan Barnych, Zhui Tu, Wenjie Ren, Zhenfeng Li (5865902), Debin Wan, Qinghua He, Zhenfeng Li, Yanping Li   +19 more
core   +1 more source

Microbial Metabolite‐Stimulated Bitter Taste Receptor T2R14 Signaling Is Modulated by CFTR Interactions

The FASEB Journal, Volume 40, Issue 11, 15 June 2026.
Bitter taste receptors (T2Rs) play an important role in physiological and cellular responses. Bitter tasting chemicals and microbial metabolites can bind to T2R14, which enables interactions between T2R14 and specific intracellular domains of the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel.
Tejas Gupte, Nisha Singh, Vikram Bhatia, Kavisha Arora, Shayan Amiri, Paul Fernyhough, Anjaparavanda P. Naren, Shyamala Dakshinamurti, Prashen Chelikani   +8 more
wiley   +1 more source

Lysosomal cholesterol export reconstituted from fragments of Niemann-Pick C1

eLife, 2018
Niemann-Pick C1 (NPC1) is a polytopic membrane protein with 13 transmembrane helices that exports LDL-derived cholesterol from lysosomes by carrying it through the 80 Å glycocalyx and the 40 Å lipid bilayer. Transport begins when cholesterol binds to the
Michael Nguyen Trinh, Michael S Brown, Joachim Seemann, Joseph L Goldstein, Feiran Lu   +4 more
doaj   +1 more source

Reduced Maintenance DNA Methylation Thresholds Enable Sensitive Reporter Assays for UHRF1 and DNMT1 Inhibition

Advanced Science, Volume 13, Issue 32, 9 June 2026.
The authors engineer colorectal cancer cells with reduced redundancy of UHRF1 and DNMT1, key DNA methylation maintenance factors, lowering the maintenance threshold and sensitizing cells to inhibition. They develop reporter assays driven by endogenous tumor‐suppressor gene promoters with enhanced sensitivity and dynamic range to demethylating drugs ...
Cuicui Xia, Ying Cui, Jiongliang Wang, Wenjing Bai, Danyang Wang, Yao Chen, Huahui Guo, Liangyi Zong, Bing Qin, Xintong Cai, Jie Wang, Jing Huang, Scott B. Rothbart, Zufeng Guo, Stephen B. Baylin, Xiangqian Kong   +15 more
wiley   +1 more source

Cells emerging from the screen are less susceptible to infection with C. parvum.

, 2022
The population of Cas9-Clone K expressing cells transduced with the library prior to the screen were compared to the population following the last round of selection.
Boris Striepen (86887), Alexis R. Gibson (12563046), Daniel P. Beiting (7329887), Jodi A. Gullicksrud (12563055), Julie B. Engiles (9756268), Keenan M. O’Dea (12700490), John G. Doench (9465667), Christopher A. Hunter (142425), Adam Sateriale (504694), Ryan D. Pardy (3775132), Jennifer E. Dumaine (12563049)   +10 more
core   +1 more source

A cautionary note on the potential pitfalls of using N-terminal truncated CD63 to label small extracellular vesicles

Scientific Reports
Small extracellular vesicles (sEV) are nanosized vesicles that facilitate intracellular communication. A significant research obstacle is the isolation of sEV devoid of non-sEV contaminants.
Elias Sulaiman, Derek M. Yellon, Sean M. Davidson   +2 more
doaj   +1 more source

NanoLuX: a network-wide Nanoluciferase-based platform to monitor activation of classical and atypical chemokine receptors

, 2019
The activity of chemokine receptors is dependent on G proteins that, upon chemokine binding, trigger various intracellular signalling cascades, as well as on b-arrestins that, following receptor phosphorylation by GRK, orchestrate its desensitization,
Ollert, Markus, Szpakowska, Martyna, Meyrath, Max Marc Roger, Counson, Manuel, Beaupain, Nadia, Reynders, Nathan, Chevigne, Andy   +6 more
core   +1 more source