Results 61 to 70 of about 2,893 (161)

Scalable Engineering of Bio‐Manufactured Extracellular Vesicles for Selective Delivery in Ovarian Cancer Patient‐Derived Models

Advanced Science, Volume 13, Issue 40, 17 July 2026.
Engineered extracellular vesicles displaying Ephrin‐B2 selectively target Ephrin‐B4–expressing ovarian cancer cells, enabling precise delivery in patient‐derived models. This scalable bio‐manufacturing platform reveals a versatile strategy to exploit Ephrin signaling for highly specific therapeutic payload delivery and motivates exploration of tailored
Nihar Godbole, Andrew Lai, Alexander Quinn, Marianne Gillard, Dominic Guanzon, Katherin Scholz‐Romero, Alexis Salas‐Burgos, Bastián Lillo Dapremont, Rohan Lourie, Naven Chetty, Richard Lobb, Kate Beecher, Amy E. McCart Reed, Kaltin Ferguson, Biao Sun, Yaowu He, John D. Hooper, Carlos Salomon   +17 more
wiley   +1 more source

Organometallic Complex Strongly Impairs Chikungunya Virus Entry to the Host Cells

Frontiers in Microbiology, 2020
Chikungunya fever is a disease caused by the Chikungunya virus (CHIKV) that is transmitted by the bite of the female of Aedes sp. mosquito. The symptoms include fever, muscle aches, skin rash, and severe joint pains.
Débora Moraes de Oliveira, Igor de Andrade Santos, Daniel Oliveira Silva Martins, Daniel Oliveira Silva Martins, Yasmim Garcia Gonçalves, Léia Cardoso-Sousa, Robinson Sabino-Silva, Gustavo Von Poelhsitz, Eduardo de Faria Franca, Nilson Nicolau-Junior, Carolina Colombelli Pacca, Carolina Colombelli Pacca, Andres Merits, Mark Harris, Ana Carolina Gomes Jardim, Ana Carolina Gomes Jardim   +15 more
doaj   +1 more source

Integrated Immunological Engineering Platform for Scalable Production of iPSC‐Derived Megakaryocytes

HLA, Volume 108, Issue 1, July 2026.
ABSTRACT Severe thrombocytopenia and impaired platelet function remain major clinical challenges in hematologic diseases, chemotherapy, bone marrow failure, surgery and obstetric haemorrhage. Conventional platelet transfusions are limited by donor dependency, short shelf life, storage constraints, and immune‐mediated platelet refractoriness caused ...
Rabea Dettmer, Alice Rovai, Linus Schröder, Thea Reinkens, Willem Wolkers, Rainer Blasczyk, Constanca Figueiredo   +6 more
wiley   +1 more source

Rapid and Wash-Free Anti-Drug Antibody Assay Enabled by a Complementary Nanoluciferase Biosensor

Analytical Chemistry
Abstract Biotherapeutics have demonstrated remarkable therapeutic efficacy across a wide range of diseases; however, their clinical application is often hindered by the development of anti-drug antibodies (ADAs). Traditionally, ADA monitoring has relied on ligand-binding assays (LBAs) such as enzyme-linked immunosorbent assays (ELISA ...
Ruoxuan Sun, Mark G. Qian, Xiaobin Zhang
openaire   +2 more sources

A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays

Communications Biology, 2023
Transgenic luciferase-expressing Plasmodium falciparum parasites have been widely used for the evaluation of anti-malarial compounds. Here, to screen for anti-malarial drugs effective against multiple stages of the parasite, we generate a P.
Yukiko Miyazaki, Martijn W. Vos, Fiona J. A. Geurten, Pierre Bigeard, Hans Kroeze, Shohei Yoshioka, Mitsuhiro Arisawa, Daniel Ken Inaoka, Valerie Soulard, Koen J. Dechering, Blandine Franke-Fayard, Shinya Miyazaki   +11 more
doaj   +1 more source

A novel TOX-nanoluciferase reporter mouse for exploring modulators of T cell exhaustion

The Journal of Immunology
Abstract Cytotoxic T cell (CTL) exhaustion is driven by chronic T cell receptor (TCR) stimulation, leading to a dysfunctional state of cells. Exhausted CTLs exhibit diminished effector function against chronic infections and cancers. Therefore, reducing CTL exhaustion may re-establish effective adaptive immune responses.
Li, Ling, Mueller, Yvonne M., Hioki, Kou, den Dekker, Renske J.H., Brouwers-Haspels, Inge, Mezzanotte, Laura, Maas, Alex, Erkeland, Stefan, Katsikis, Peter D.   +8 more
openaire   +3 more sources

A nano-luciferase expressing human coronavirus OC43 for countermeasure development

Virus Research
The genetic diversity of the coronavirus (CoV) family poses a significant challenge for drug discovery and development. Traditional antiviral drugs often target specific viral proteins from specific viruses which limits their use, especially against ...
Meghan V. Diefenbacher, Thomas J. Baric, David R. Martinez, Ralph S. Baric, Nicholas J. Catanzaro, Timothy P. Sheahan   +5 more
doaj   +1 more source

Microbial Metabolite‐Stimulated Bitter Taste Receptor T2R14 Signaling Is Modulated by CFTR Interactions

The FASEB Journal, Volume 40, Issue 11, 15 June 2026.
Bitter taste receptors (T2Rs) play an important role in physiological and cellular responses. Bitter tasting chemicals and microbial metabolites can bind to T2R14, which enables interactions between T2R14 and specific intracellular domains of the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel.
Tejas Gupte, Nisha Singh, Vikram Bhatia, Kavisha Arora, Shayan Amiri, Paul Fernyhough, Anjaparavanda P. Naren, Shyamala Dakshinamurti, Prashen Chelikani   +8 more
wiley   +1 more source

A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging

PLOS ONE, 2018
Chagas disease, caused by the intracellular protozoan Trypanosoma cruzi, affects 8-10 million people worldwide and represents a major public health challenge. There is no effective treatment or vaccine to control the disease that is characterized by a mild acute phase followed by a chronic life-long infection.
Erica Silberstein, Carylinda Serna, Stenio Perdigão Fragoso, Rana Nagarkatti, Alain Debrabant   +4 more
openaire   +4 more sources

Reduced Maintenance DNA Methylation Thresholds Enable Sensitive Reporter Assays for UHRF1 and DNMT1 Inhibition

Advanced Science, Volume 13, Issue 32, 9 June 2026.
The authors engineer colorectal cancer cells with reduced redundancy of UHRF1 and DNMT1, key DNA methylation maintenance factors, lowering the maintenance threshold and sensitizing cells to inhibition. They develop reporter assays driven by endogenous tumor‐suppressor gene promoters with enhanced sensitivity and dynamic range to demethylating drugs ...
Cuicui Xia, Ying Cui, Jiongliang Wang, Wenjing Bai, Danyang Wang, Yao Chen, Huahui Guo, Liangyi Zong, Bing Qin, Xintong Cai, Jie Wang, Jing Huang, Scott B. Rothbart, Zufeng Guo, Stephen B. Baylin, Xiangqian Kong   +15 more
wiley   +1 more source