Results 41 to 50 of about 2,893 (161)

Bioluminescent and fluorescent reporter-expressing recombinant Akabane virus (AKAV): an excellent tool to dissect viral replication

Frontiers in Microbiology
IntroductionAkabane virus (AKAV) is a worldwide epidemic arbovirus belonging to the Bunyavirales order that predominantly infects livestock and causes severe congenital malformations.
Jingjing Liu, Jingjing Liu, Fang Wang, Jiangang Zhao, Jiangang Zhao, Yinglin Qi, Jitao Chang, Jitao Chang, Chao Sun, Zhigang Jiang, Junwei Ge, Xin Yin   +11 more
doaj   +1 more source

A cell-free platform to measure coronavirus membrane fusion

STAR Protocols, 2023
Summary: Here we present a protocol to measure coronavirus-mediated membrane fusion, an essential event in coronavirus cell entry. The approach uses nanoluciferase (Nluc) “HiBiT”-tagged corona virus-like particles (VLPs) and Nluc “LgBiT”-containing ...
Thomas Kicmal, Enya Qing, Grant M. Hawkins, Alexandria Wilcox, Tom Gallagher   +4 more
doaj   +1 more source

Trimerization and Genotype–Phenotype Correlation of COL4A5 Mutants in Alport Syndrome

Kidney International Reports, 2020
Introduction: Alport syndrome is a hereditary glomerulonephritis that results from the disruption of collagen α345(IV) heterotrimerization caused by mutation in COL4A3, COL4A4 or COL4A5 genes. Many clinical studies have elucidated the correlation between
Misato Kamura, Tomohiko Yamamura, Kohei Omachi, Mary Ann Suico, Kandai Nozu, Shota Kaseda, Jun Kuwazuru, Tsuyoshi Shuto, Kazumoto Iijima, Hirofumi Kai   +9 more
doaj   +1 more source

RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System

Advanced Science, EarlyView.
Condensates at synapses organize synaptic vesicles (SVs) and are crucial for efficient neurotransmitter release, yet how RNA contributes to this mesoscale architecture remains poorly understood. Here we uncover that RNA modulates synapsin–driven condensates.
Branislava Rankovic, Zachary M. Geisterfer, Akshita Chhabra, Vladimir M. Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, Jingao Sun, Kiara Freitag, Katja Nowick, Marina Jendrach, Frank L. Heppner, Vladimir Despic, Michaela Müller‐McNicoll, Stephan Sigrist, Geert van den Bogaart, Sofiia Reshetniak, Amy S. Gladfelter, Dragomir Milovanovic   +21 more
wiley   +1 more source

A luminescent attenuated SARS-CoV-2 for the identification and validation of drug-resistant mutants

Journal of Virology
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has necessitated a continuous updating of vaccines. In contrast, antivirals remained effective as they target conserved viral proteins that are essential for the viral
Yao Ma, Chengjin Ye, Ahmed Magdy Khalil, Sara H. Mahmoud, Elizabeth B. Sobolik, Alexander L. Greninger, Esteban Castro, Nathaniel Jackson, Mahmoud Bayoumi, Richard K. Plemper, Luis Martinez-Sobrido   +10 more
doaj   +1 more source

Von In‐Silico bis In‐Cerebro – Entwicklung des Orexinrezeptor Antagonisten „Photorexant“ zur Photomodulation In Vitro und im Gehirn der Maus

Angewandte Chemie, EarlyView.
Durch einen strukturbasierten Designansatz wurden photoschaltbare Derivate des von der FDA zugelassenen Arzneistoffes Suvorexant entwickelt, gedockt, synthetisiert und pharmakologisch an den Orexin‐1‐ und 2‐Rezeptoren (OX1R und OX2R) charakterisiert.
Marcus Angermann, Marie H. Deventer, Vicente Ledesma‐Martin, Nuno Martinho, Alix Flake, Silvia Mori, Angel Nuñez, Jaime Pignatelli, Rita C. Guedes, Christophe P. Stove, Michael Decker   +10 more
wiley   +1 more source

Modelling Absorption Spectra of Furimamide-Nanoluciferase System

, 2022
Bioluminescence is a biological phenomenon of light production by living species with many applications[1], notably for the detection of cancer cells[2]. The present study deals with a ligand-protein bioluminescent system (furimazine-Nanoluciferase) derived from a shrimp called Oplophorus gracilirostri with a very high luminescence intensity[3].
Moumene, Houda, Mangaud, Etienne, Navizet, Isabelle   +2 more
openaire   +1 more source

Bioluminescent reporter influenza A viruses to track viral infections

Microbiology Spectrum
Influenza A viruses (IAVs) infect a wide range of mammalian and bird species and are responsible for seasonal outbreaks and occasional pandemics of great consequences in humans.
Ramya S. Barre, Ahmed Mostafa, Kevin Chiem, Rebecca L. Pearl, Roy N. Platt, Anastasija Cupic, Timothy J. C. Anderson, Ulla G. Knaus, Randy A. Albrecht, Adolfo García-Sastre, James J. Kobie, Aitor Nogales, Luis Martinez-Sobrido   +12 more
doaj   +1 more source

In Silico to In Cerebro—Development of the Orexin Receptor Antagonist “Photorexant” for Photomodulation In Vitro and in Mouse Brains

Angewandte Chemie International Edition, EarlyView.
Following a structure‐based design approach, photoswitchable derivatives of the FDA‐approved drug suvorexant were designed, docked, synthesized, and characterized pharmacologically at the orexin 1 and 2 receptors (OX1R and OX2R). Optimized “photorexant” showed up to an 11‐fold difference in IC50 between photoisomers, enabled dynamic and reversible ...
Marcus Angermann, Marie H. Deventer, Vicente Ledesma‐Martin, Nuno Martinho, Alix Flake, Silvia Mori, Angel Nuñez, Jaime Pignatelli, Rita C. Guedes, Christophe P. Stove, Michael Decker   +10 more
wiley   +1 more source

Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy. [PDF]

PLoS ONE, 2018
Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most
JingZhe Li, ZhiLan Guo, Takashi Sato, Bo Yuan, YanYan Ma, Dan Qian, JuYing Zhong, MengMeng Jin, Peng Huang, LuYang Che, Yi Wang, Yan Lei, ChangZhen Liu   +12 more
doaj   +1 more source