Results 41 to 50 of about 3,520 (152)
Bioluminescent reporter influenza A viruses to track viral infections
Microbiology SpectrumInfluenza A viruses (IAVs) infect a wide range of mammalian and bird species and are responsible for seasonal outbreaks and occasional pandemics of great consequences in humans.
Ramya S. Barre +12 more
doaj +1 more source
Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy. [PDF]
PLoS ONE, 2018 Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most
JingZhe Li +12 more
doaj +1 more source
The c‐Src inhibitor eCF506 diminishes opioid tolerance and reduces β‐arrestin2 recruitment
British Journal of Pharmacology, EarlyView.Morphine activation of μ receptors causes tolerance through c‐Src activation and β‐arrestin2 recruitment. c‐Src inhibition or degradation reduces β‐arrestin2 recruitment, μ receptor endocytosis, while causing receptor upregulation, all likely contributing to reduced morphine tolerance.
Samuel Singleton +6 more
wiley +1 more source
Transgenic selection and underlying mechanisms in apicomplexan parasites
The FEBS Journal, EarlyView.Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju +2 more
wiley +1 more source
Role of Extracellular Vesicles in Shaping Immune Responses in the Lymph Node Microenvironment
Biology of the Cell, Volume 118, Issue 10, October 2026.Functional alliances of stromal, innate and adaptive immune cells in EV immunity in the lymph node. EV reflect the phenotype of their mother cell and cluster material from their surroundings. In the lymph node, EV preserve valuable context information between multiple cellular effectors to coordinate immunity in adaption to change.
Alicia Charrier +4 more
wiley +1 more source
A novel nanoluciferase transgenic reporter to measure proteinuria in zebrafish
, 2021 The zebrafish is an important animal system for modelling human diseases. This includes kidney dysfunction as the embryonic kidney (pronephros) shares considerable molecular and morphological homology with the human nephron.
Naylor, Richard W.; id_orcid +6 more
core +1 more source
New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
PPAR Research, 2017 The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development.
Séverine A. Degrelle +2 more
doaj +1 more source
iScience, 2023 Summary: E-selectin is expressed on endothelial cells in response to inflammatory cytokines and mediates leukocyte rolling and extravasation. However, studies have been hampered by lack of experimental approaches to monitor expression in real time in ...
Lydia Ogrodzinski +7 more
doaj +1 more source
A Sensitive Reporter Mouse Model to Study Adipocyte‐Derived Extracellular Vesicles In Vivo
Journal of Extracellular VesiclesExtracellular vesicles (EVs) affect the function of cells in living animals. Yet, cell type‐specific EV abundances and their distribution in biological fluids are technically challenging to study.
Didde Riisager Hansen +3 more
doaj +1 more source
Engineered reporter phages for detection of Escherichia coli, Enterococcus, and Klebsiella in urine
Nature Communications, 2023 The rapid detection and species-level differentiation of bacterial pathogens facilitates antibiotic stewardship and improves disease management. Here, we develop a rapid bacteriophage-based diagnostic assay to detect the most prevalent pathogens causing ...
Susanne Meile +22 more
doaj +1 more source