Results 31 to 40 of about 2,893 (161)

Incorporation of reporter genes into mitochondrial DNA in budding yeast

STAR Protocols, 2022
Summary: Many aspects of mitochondrial gene expression are still unknown, which can be attributed to limitations in molecular tools. Here, we present a protocol to introduce reporter genes into the mitochondrial genome of budding yeast, Saccharomyces ...
Magdalena Rzepka, Tamara Suhm, Martin Ott   +2 more
doaj   +1 more source

Glutathione‐Responsive Acyl‐Modifications for Targeted RNA Decaging and Prolonged Protein Synthesis

Angewandte Chemie, EarlyView.
The self‐immolation of disulfide‐based mRNA modifications in response to endogenous glutathione (GSH) promotes a gradual release of translatable mRNA within the cell, leading to improved nuclease resistance, tunable release properties, and a significant increase (up to 600%) of target protein production over time. This strategy offers an exciting proof
Mary E. Flood, Sathishkumar Kurusamy, Mark Berney, C. Mark Smales, Elizabeth M. Topp, Joanna F. McGouran, Steven Ferguson   +6 more
wiley   +2 more sources

Periplasmic Nanobody-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus

Viruses, 2019
We explore evolved soybean ascorbate peroxidase (APEX2) as a reporter when fused to the C-termini of llama nanobodies (single-domain antibodies, sdAb; variable domains of heavy chain-only antibodies, VHH) targeted to the E. coli periplasm.
Laura J. Sherwood, Andrew Hayhurst
doaj   +1 more source

A nanoluciferase-tagged Schmallenberg virus (SBV): an efficient tool for measuring and tracking viral infection dynamics. [PDF]

J Med Microbiol
Introduction. Schmallenberg virus (SBV) is an arthropod-borne virus and belongs to the Simbu serogroup within the family Peribunyaviridae, genus Orthobunyavirus. Infection of naïve ruminants at critical stages of gestation can result in severe congenital malformations or abortion.
Sick F, Aebischer A, Beer M, Wernike K.
europepmc   +3 more sources

A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19

Nature Communications, 2020
A high-throughput platform would greatly facilitate coronavirus disease 2019 (COVID-19) serological testing and antiviral screening. To address this, Shi and colleagues present a high-throughput nanoluciferase severe respiratory syndrome coronavirus 2 ...
Xuping Xie, Antonio E. Muruato, Xianwen Zhang, Kumari G. Lokugamage, Camila R. Fontes-Garfias, Jing Zou, Jianying Liu, Ping Ren, Mini Balakrishnan, Tomas Cihlar, Chien-Te K. Tseng, Shinji Makino, Vineet D. Menachery, John P. Bilello, Pei-Yong Shi   +14 more
doaj   +1 more source

Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and Replication

Viruses, 2022
The COVID-19 pandemic continues to threaten healthcare systems worldwide due to the limited access to vaccines, suboptimal treatment options, and the continuous emergence of new and more transmissible SARS-CoV-2 variants.
Ulrik Fahnøe, Long V. Pham, Carlota Fernandez-Antunez, Rui Costa, Lizandro René Rivera-Rangel, Andrea Galli, Shan Feng, Lotte S. Mikkelsen, Judith M. Gottwein, Troels K. H. Scheel, Santseharay Ramirez, Jens Bukh   +11 more
doaj   +1 more source

Novel repertoire of tau biosensors to monitor pathological tau transformation and seeding activity in living cells

eLife, 2023
Aggregates of the tau protein are a well-known hallmark of several neurodegenerative diseases, collectively referred to as tauopathies, including frontal temporal dementia and Alzheimer’s disease (AD).
Erika Cecon, Atsuro Oishi, Marine Luka, Delphine Ndiaye-Lobry, Arnaud François, Mathias Lescuyer, Fany Panayi, Julie Dam, Patricia Machado, Ralf Jockers   +9 more
doaj   +1 more source

218. Monitoring ER Stress Activation of the ATF6 Pathway Using Nanoluciferase [PDF]

Molecular Therapy, 2016
The endoplasmic reticulum (ER) is an invaluable component of the secretory pathway; serving as the main site for protein synthesis and modification, lipid metabolism, lipoprotein secretion, and calcium homeostasis. In response to “stress”, the ER maintains homeostasis through the unfolded protein response (UPR). The UPR is an adaptive response employed
Emily S. Wires, Doug Howard, Mark J. Henderson, Xiaokang Yan, Kathleen A. Trychta, Emily J. Heathward, Yajun Zhang, Molly Lutrey, Christopher Richie, Brandon K. Harvey   +9 more
openaire   +1 more source

Are we serologically prepared against an avian influenza pandemic and could seasonal flu vaccines help us?

mBio
The current situation with H5N1 highly pathogenic avian influenza virus (HPAI) is causing a worldwide concern due to multiple outbreaks in wild birds, poultry, and mammals. Moreover, multiple zoonotic infections in humans have been reported. Importantly,
Iván Sanz-Muñoz, Javier Sánchez-Martínez, Carla Rodríguez-Crespo, Corina S. Concha-Santos, Marta Hernández, Silvia Rojo-Rello, Marta Domínguez-Gil, Ahmed Mostafa, Luis Martinez-Sobrido, Jose M. Eiros, Aitor Nogales   +10 more
doaj   +1 more source

CRISPR-Mediated Protein Tagging with Nanoluciferase to Investigate Native Chemokine Receptor Function and Conformational Changes [PDF]

Cell Chemical Biology, 2020
G protein-coupled receptors are a major class of membrane receptors that mediate physiological and pathophysiological cellular signaling. Many aspects of receptor activation and signaling can be investigated using genetically encoded luminescent fusion proteins. However, the use of these biosensors in live cell systems requires the exogenous expression
Carl W. White, Birgit Caspar, Hannah K. Vanyai, Kevin D.G. Pfleger, Stephen J. Hill   +4 more
openaire   +2 more sources