Results 21 to 30 of about 2,893 (161)
A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
Nature Communications, 2021 Serological tests are important diagnostic and disease surveillance tools for addressing the COVID-19 pandemic. Here, the authors present a tri-part Nanoluciferase based assay to detect antibodies against SARS-CoV-2.
Zhong Yao +23 more
doaj +1 more source
A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
Communications Biology, 2022 Membrane protein trafficking is monitored using split nanoluciferase. Receptor internalization leads to complementation on the early endosome and a bioluminescent response, and is applied to receptor internalization/recycling, antibody-mediated ...
Arfaxad Reyes-Alcaraz +5 more
doaj +1 more source
Advancing Luciferase-Based Antibody Immunoassays to Next-Generation Mix and Read Testing
Biosensors, 2023 Antibody measurements play a central role in the diagnosis of many autoimmune and infectious diseases. One antibody detection technology, Luciferase Immunoprecipitation Systems (LIPS), utilizes genetically encoded recombinant luciferase antigen fusion ...
Peter D. Burbelo +2 more
doaj +1 more source
Nanoluciferase Assay in C. elegans v1 [PDF]
, 2019 A highly sensitive Nanoluciferase (NanoLuc)-based method in multi-well format to detect constitutive and inducible gene expression in whole C. elegans. Worms have to be lysed for this end-point assay, no spatial resolution of expression can be detected with this protocol.
Emily Troemel +3 more
openaire +1 more source
EndoBind detects endogenous protein-protein interactions in real time
Communications Biology, 2021 Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system.
Anke Bill +12 more
doaj +1 more source
Revisiting Hydrazine Moiety as a Scaffold for the Synthesis of Potential Antiviral Pyrazole Ribonucleoside Analogues. [PDF]
ChemMedChemThree series of pyrazole ribonucleoside analogues were designed and synthesized. They were evaluated, along with some of their pronucleotides, for their antiviral activity against RNA viruses. Pyrazole ribonucleoside analogues were prepared via a linear approach starting with the reaction of D‐ribosylhydrazine with 1,3‐dicarbonyl or acrylonitrile ...
Guillou A +6 more
europepmc +2 more sources
NanoLuciferase technology-based detection of TMPRSS2 as attempt to develop anti-coronavirus agents
Biochemistry and Biophysics Reports•Utilized NanoBiT technology for high-throughput screening.•Identified compounds reducing TMPRSS2 expression, a crucial step for SARS-CoV-2 entry.•Explored a broad range of compound libraries.
Yanwen Chen +5 more
doaj +3 more sources
The Journal of Physical Chemistry B, 2020 Luminescence of furimamide is 150 times brighter than oxidized luciferins in firefly and renilla luciferase. However, we do not have a clear understanding of the structure, function, and dynamic behavior of the nanoluciferase-furimamide complex. Here, for the first time, the absorption and emission properties of eight different possible light emitter ...
Sahihi, Mehdi +2 more
openaire +2 more sources
Nghiên cứu tạo protein tín hiệu nanoluciferase: Ứng dụng tạo cảm biến sinh học nhận diện kháng sinh
Tạp chí Khoa học Đại học Cần Thơ, 2020 Nghiên cứu được thực hiện nhằm tổng hợp protein tín hiệu nanoluciferase trong điều kiện phòng thí nghiệm (in vitro), sử dụng hệ thống phiên mã dịch mã ngoài tế bào (cell-free synthesis) để ứng dụng tạo cảm biến sinh học nhận diện nhóm kháng sinh ức chế ...
Trần Thị Mỹ Duyên +1 more
doaj +3 more sources
Journal of Molecular Cell Biology, 2022 Abstract The coronavirus disease 2019 (COVID-19) pandemic has progressed over 2 years since its onset causing significant health concerns all over the world and is currently curtailed by mass vaccination. Immunity acquired against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be following either infection or ...
Jeremiah, Sundararaj Stanleyraj +2 more
openaire +2 more sources