Results 11 to 20 of about 2,893 (161)

A nanoluciferase biosensor to investigate endogenous chemokine secretion and receptor binding [PDF]

iScience, 2021
Summary: Secreted chemokines are critical mediators of cellular communication that elicit intracellular signaling by binding membrane-bound receptors. Here we demonstrate the development and use of a sensitive real-time approach to quantify secretion and
Carl W. White, Laura E. Kilpatrick, Kevin D.G. Pfleger, Stephen J. Hill   +3 more
doaj   +3 more sources

Dynamic High-Sensitivity Quantitation of Procollagen-I by Endogenous CRISPR-Cas9 NanoLuciferase Tagging [PDF]

Cells, 2020
The ability to quantitate a protein of interest temporally and spatially at subcellular resolution in living cells would generate new opportunities for research and drug discovery, but remains a major technical challenge.
Ben C. Calverley, Karl E. Kadler, Adam Pickard   +2 more
doaj   +4 more sources

A novel nanoluciferase transgenic reporter measures proteinuria in zebrafish [PDF]

Kidney International, 2021
Abstract The zebrafish is an important animal system for modelling human diseases. This includes kidney dysfunction as the embryonic kidney (pronephros) shares considerable molecular and morphological homology with the human nephron. Zebrafish also have a high fecundity, with females capable of laying 200-300 eggs per
Richard W. Naylor, Emmanuel Lemarie, Anthony Jackson-Crawford, J. Bernard Davenport, Aleksandr Mironov, Martin Lowe, Rachel Lennon   +6 more
openaire   +4 more sources

Nanoluciferase-Based Method for Detecting Gene Expression in Caenorhabditis elegans [PDF]

Genetics, 2019
Abstract Genetic reporters such as the green fluorescent protein (GFP) can facilitate measurement of promoter activity and gene expression. However, animal autofluorescence limits the sensitivity of GFP and other fluorescent reporters in whole-animal settings like in the nematode Caenorhabditis elegans.
Sfarcic, Ivana, Bui, Theresa, Daniels, Erin C, Troemel, Emily R   +3 more
openaire   +6 more sources

Construction and Generation of a Recombinant Senecavirus a Stably Expressing the NanoLuc Luciferase for Quantitative Antiviral Assay

Frontiers in Microbiology, 2021
Senecavirus A (SVA), also known as Seneca Valley virus, is a recently emerged picornavirus that can cause swine vesicular disease, posing a great threat to the global swine industry.
Xiaoran Guo, Kuan Zhao, Xiaona Liu, Baishi Lei, Wuchao Zhang, Xiuli Li, Wanzhe Yuan, Wanzhe Yuan, Wanzhe Yuan   +8 more
doaj   +1 more source

Sweet and Bright: Illuminating Glycoprotein-Mediated Endocytosis via Metabolic Labeling and NanoLuciferase. [PDF]

ACS Chem Biol
Peer ...
Soliman MO, Koivuniemi A, Freiburghaus V, Garbujo S, Urech L, Frascotti G, Prosperi D, Colombo M, Hanzlikova M, Hartrampf N, Laaksonen T, Wang S.   +11 more
europepmc   +3 more sources

Investigation of adenosine A1 receptor-mediated β-arrestin 2 recruitment using a split-luciferase assay

Frontiers in Pharmacology, 2023
Background: Adenosine A1 receptor (A1AR) plays a prominent role in neurological and cardiac diseases and inflammatory processes. Its endogenous ligand adenosine is known to be one of the key players in the sleep–wake cycle.
Luisa Saecker, Hanns Häberlein, Sebastian Franken   +2 more
doaj   +1 more source

Protocol to quantify glycan-mediated binding between viral particles and human peripheral blood mononuclear cells using nanoluciferase

STAR Protocols, 2023
Summary: Prior to receptor engagement, a specific, non-electrostatic glycan-glycan interaction between viral particles and host cells may lengthen the dwell time of the virus at the cellular surface, thereby facilitating subsequent virus entry.
Belinda L. Spillings, Johnson Mak
doaj   +1 more source

A Nanoluciferase biosensor to investigate endogenous chemokine secretion and receptor binding [PDF]

, 2020
Summary Secreted chemokines are critical mediators of cellular communication that elicit intracellular signalling by binding membrane-bound receptors. Here we demonstrate the development and use of a sensitive real-time approach to quantify secretion and receptor binding of native chemokines in live cells to better understand their ...
Carl W. White, Kevin D. G. Pfleger, Stephen J. Hill   +2 more
openaire   +1 more source

Nanobody-Nanoluciferase Fusion Protein-Enabled Immunoassay for Ochratoxin A in Coffee with Enhanced Specificity and Sensitivity

Toxins, 2022
Ochratoxin A (OTA), one of the best-known mycotoxins, causes problems concerning food safety with potential toxic effects in humans and animals. So, it is crucial to develop simple and sensitive methods for the detection of OTA. Herein, a nanoluciferase–nanobody fusion protein (Nb28-Nluc)-retaining antibody recognition and enzymatic activity was first ...
Kunlu Bao, Xing Liu, Yujing Liao, Zilong Liu, Hongmei Cao, Long Wu, Qi Chen   +6 more
openaire   +8 more sources