Results 11 to 20 of about 3,520 (152)
STAR Protocols, 2023 Summary: Prior to receptor engagement, a specific, non-electrostatic glycan-glycan interaction between viral particles and host cells may lengthen the dwell time of the virus at the cellular surface, thereby facilitating subsequent virus entry.
Belinda L. Spillings, Johnson Mak
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A nanoluciferase biosensor to investigate endogenous chemokine secretion and receptor binding [PDF]
iScience, 2021 Summary: Secreted chemokines are critical mediators of cellular communication that elicit intracellular signaling by binding membrane-bound receptors. Here we demonstrate the development and use of a sensitive real-time approach to quantify secretion and
Carl W. White +3 more
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Nature Communications, 2020 A high-throughput platform would greatly facilitate coronavirus disease 2019 (COVID-19) serological testing and antiviral screening. To address this, Shi and colleagues present a high-throughput nanoluciferase severe respiratory syndrome coronavirus 2 ...
Xuping Xie +14 more
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Nghiên cứu tạo protein tín hiệu nanoluciferase: Ứng dụng tạo cảm biến sinh học nhận diện kháng sinh [PDF]
Tạp chí Khoa học Đại học Cần Thơ, 2020 Nghiên cứu được thực hiện nhằm tổng hợp protein tín hiệu nanoluciferase trong điều kiện phòng thí nghiệm (in vitro), sử dụng hệ thống phiên mã dịch mã ngoài tế bào (cell-free synthesis) để ứng dụng tạo cảm biến sinh học nhận diện nhóm kháng sinh ức chế ...
Trần Thị Mỹ Duyên +1 more
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Biochemistry and Biophysics ReportsLuciferase complementation assays have emerged as a simple means of monitoring receptor-effector interactions in living cells in a time-resolved manner.
Viktor Burström +5 more
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Cells, 2020 The ability to quantitate a protein of interest temporally and spatially at subcellular resolution in living cells would generate new opportunities for research and drug discovery, but remains a major technical challenge.
Ben C. Calverley +2 more
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Modelling Absorption Spectra of Furimamide-Nanoluciferase System
, 2022 International audienceBioluminescence is a biological phenomenon of light production by living species with many applications[1], notably for the detection of cancer cells[2].
Moumene, Houda +2 more
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A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
Nature Communications, 2021 Serological tests are important diagnostic and disease surveillance tools for addressing the COVID-19 pandemic. Here, the authors present a tri-part Nanoluciferase based assay to detect antibodies against SARS-CoV-2.
Zhong Yao +23 more
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A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
Communications Biology, 2022 Membrane protein trafficking is monitored using split nanoluciferase. Receptor internalization leads to complementation on the early endosome and a bioluminescent response, and is applied to receptor internalization/recycling, antibody-mediated ...
Arfaxad Reyes-Alcaraz +5 more
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Advancing Luciferase-Based Antibody Immunoassays to Next-Generation Mix and Read Testing
Biosensors, 2023 Antibody measurements play a central role in the diagnosis of many autoimmune and infectious diseases. One antibody detection technology, Luciferase Immunoprecipitation Systems (LIPS), utilizes genetically encoded recombinant luciferase antigen fusion ...
Peter D. Burbelo +2 more
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