Results 1 to 10 of about 2,824 (109)

Construction of a Recombinant Porcine Epidemic Diarrhea Virus Encoding Nanoluciferase for High-Throughput Screening of Natural Antiviral Products

Viruses, 2021
Porcine epidemic diarrhea virus (PEDV) is the predominant cause of an acute, highly contagious enteric disease in neonatal piglets. There are currently no approved drugs against PEDV infection.
Qigai He, Rui Luo
exaly   +3 more sources

Construction, Characterization and Application of Recombinant Porcine Deltacoronavirus Expressing Nanoluciferase

Viruses, 2021
Porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus, causes diarrhoea in suckling piglets and has the potential for cross-species transmission. No effective PDCoV vaccines or antiviral drugs are currently available.
Shaobo Xiao, Liurong Fang, Puxian Fang   +2 more
exaly   +3 more sources

Surrogate Virus Neutralisation Test Based on Nanoluciferase-Tagged Antigens to Quantify Inhibitory Antibodies against SARS-CoV-2 and Characterise Omicron-Specific Reactivity in a Vaccination Cohort

Vaccines, 2023
Virus-specific antibodies are crucial for protective immunity against SARS-CoV-2. Assessing functional antibodies through conventional or pseudotyped virus neutralisation tests (pVNT) requires high biosafety levels.
Stephan Ludwig, Eva Lorentzen, Eike R Hrincius   +2 more
exaly   +3 more sources

NanoFIRE: A NanoLuciferase and Fluorescent Integrated Reporter Element for Robust and Sensitive Investigation of HIF and Other Signalling Pathways

Biomolecules, 2023
The Hypoxia Inducible Factor (HIF) transcription factors are imperative for cell adaption to low oxygen conditions and development; however, they also contribute to ischaemic disease and cancer.
Michael Beard, Darryl Russell, Daniel Peet   +2 more
exaly   +3 more sources

Development of a novel NanoBRET high-throughput drug screening assay for human GnRH receptor using sulfo-cyanine 5 fluorophore [PDF]

Journal of Pharmaceutical Analysis
G protein-coupled receptors (GPCRs), the largest superfamily of cell surface receptors and targets for over 30% of current clinical drugs, remain crucial for future therapeutic development.
Li Shen, Xiaozhe Du, Yakai Yang, Ming Su, Rong Rong, Jia Meng, Lee Wei Lim, David G. Fernig, Zhi-Liang Lu   +8 more
doaj   +2 more sources

Nanoluciferase Reporter Zika Viruses as Tools for Assessing Infection Kinetics and Antibody Potency

Viruses, 2023
Zika virus (ZIKV) has become endemic in multiple tropical and subtropical regions and has the potential to become widespread in countries with limited prior exposure to this infection.
Maria Rios, Evi B Struble, Krishnamurthy Konduru   +2 more
exaly   +3 more sources

A nanoluciferase complementation-based assay for monitoring β-arrestin2 recruitment to the dopamine D3 receptor [PDF]

Biochemistry and Biophysics Reports
Luciferase complementation assays have emerged as a simple means of monitoring receptor-effector interactions in living cells in a time-resolved manner.
Viktor Burström, Kuiying Xu, Emilio Garro-Martínez, Robert H. Mach, Kristoffer Sahlholm, Nibal Betari   +5 more
doaj   +2 more sources

Novel Mode of nanoLuciferase Packaging in SARS-CoV-2 Virions and VLPs Provides Versatile Reporters for Virus Production

Viruses, 2023
SARS-CoV-2 is a positive-strand RNA virus in the Coronaviridae family that is responsible for morbidity and mortality worldwide. To better understand the molecular pathways leading to SARS-CoV-2 virus assembly, we examined a virus-like particle (VLP ...
Rebekah Gullberg, Judith Frydman, Frydman Judith   +2 more
exaly   +3 more sources

A cautionary note on the potential pitfalls of using N-terminal truncated CD63 to label small extracellular vesicles [PDF]

Scientific Reports
Small extracellular vesicles (sEV) are nanosized vesicles that facilitate intracellular communication. A significant research obstacle is the isolation of sEV devoid of non-sEV contaminants.
Elias Sulaiman, Derek M. Yellon, Sean M. Davidson   +2 more
doaj   +2 more sources

A dual-reporter mouse for therapeutic discovery in Angelman syndrome [PDF]

JCI Insight
Angelman syndrome is a neurodevelopmental disorder caused by loss of the maternal UBE3A allele, the sole source of UBE3A in mature neurons owing to epigenetic silencing of the paternal allele.
Hanna Vihma, Lucas M. James, Hannah C. Nourie, Audrey L. Smith, Siyuan Liang, Carlee A. Friar, Tasmai Vulli, Lei Xing, Dale O. Cowley, Alain C. Burette, Benjamin D. Philpot   +10 more
doaj   +2 more sources