Results 71 to 80 of about 2,893 (161)
Lysosomal cholesterol export reconstituted from fragments of Niemann-Pick C1
eLife, 2018 Niemann-Pick C1 (NPC1) is a polytopic membrane protein with 13 transmembrane helices that exports LDL-derived cholesterol from lysosomes by carrying it through the 80 Å glycocalyx and the 40 Å lipid bilayer. Transport begins when cholesterol binds to the
Michael Nguyen Trinh +4 more
doaj +1 more source
Characterizing the Fate of Anti‐CS1 Nanobody Displaying Extracellular Vesicles in Multiple Myeloma
Journal of Extracellular Vesicles, Volume 15, Issue 6, June 2026.ABSTRACT Extracellular vesicles (EVs) are promising delivery vehicles capable of transporting therapeutic agents across biological barriers. However, native EVs primarily accumulate in liver, spleen and lungs, limiting targeted delivery to disease sites.
Michiel De Coster +13 more
wiley +1 more source
Development of a BRET based chloride biosensor for high throughput screening of KCC2 modulators
SLAS DiscoveryThe generation of action potentials in neuronal cells and many other physiological processes involve the transport of chloride ions. Whilst there have been advances in chloride imaging techniques utilizing FRET biosensors, there is a lack of ...
Charles S. Lay +4 more
doaj +1 more source
Nano-Luciferase complementation assay of human herpesvirus 8 chemo/cytokine-receptor interactions
Scientific ReportsSome viruses encode proteins that mimic host chemokines and/or cytokines to modulate the host immune responses against infection. Human herpesvirus 8 (HHV-8), also termed Kaposi sarcoma-associated herpesvirus (KSHV), encodes three viral CC-type ...
Vikrant Palande +2 more
doaj +1 more source
ISME CommunicationsAbstract Bacteriophages (phages) are increasingly considered for both treatment and early detection of bacterial pathogens given their specificity and rapid infection kinetics. Here, we exploit an engineered phage expressing nanoluciferase to detect signals associated with Pseudomonas aeruginosa lysis spanning single cells to populations.
Zborowsky, Sophia +7 more
openaire +4 more sources
STAR ProtocolsSummary: Neoblasts are the only cells capable of proliferation in planarians. The traditional flow cytometry protocol using Hoechst inhibits the cell cycle. Here, we present a protocol for culturing and functionally manipulating planarian neoblasts using
Wenya Zhang +3 more
doaj +1 more source
Correction to: Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction [PDF]
, 2021 Céline, Laschet, Julien, Hanson
openaire +2 more sources
Bio-ProtocolOrganoids as a drug discovery platform represent an emerging field that continues to refine its tools. NanoBRET (bioluminescence resonance energy transfer) has emerged as a proximity-based and highly sensitive assay to measure protein–protein and protein–
Hammed Badmos, Colin Steele, Ross Cagan
doaj +1 more source
Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time
Scientific Reports, 2017 Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation.
Carl W. White +4 more
doaj +1 more source
Reporter assays for HPV16 E1/E1C and E2 mRNA splicing using nanoluciferase and secreted luciferase
Journal of Virological MethodsWe have generated two reporter plasmids that either monitor expression of human papillomavirus type 16 (HPV16) E2 mRNAs using splice site SA2709 or HPV16 E1C mRNAs utilizing splice site SA2582. In plasmid pE2sLuc, the entire E2 open reading frame was replaced by the secreted luciferase (sLuc) reporter gene and in the pE1nLuc plasmid the E1/E1C open ...
Zixiao Jiang +8 more
openaire +3 more sources