Expression of nanoluciferase in transgenic TcCOL-NLuc parasites.
, 2018 (A) Luciferase activity was measured in 5 x103 live TcCOL-NLuc and TcCOL-wt cells using the Nano-Glo® Live Cell Assay System. (B) Luciferase activity was measured in lysates of 105 TcCOL-NLuc and TcCOL-wt cells using the Nano-Glo® Luciferase Assay System.
Stenio Perdigão Fragoso (218271) +4 more
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A dual-reporter mouse for therapeutic discovery in Angelman syndrome
JCI InsightAngelman syndrome is a neurodevelopmental disorder caused by loss of the maternal UBE3A allele, the sole source of UBE3A in mature neurons owing to epigenetic silencing of the paternal allele.
Hanna Vihma +10 more
doaj +1 more source
Development of a BRET based chloride biosensor for high throughput screening of KCC2 modulators
SLAS DiscoveryThe generation of action potentials in neuronal cells and many other physiological processes involve the transport of chloride ions. Whilst there have been advances in chloride imaging techniques utilizing FRET biosensors, there is a lack of ...
Charles S. Lay +4 more
doaj +1 more source
Live-Cell Identification of Inhibitors of the Lipid Transfer Protein CERT Using Nanoluciferase Bioluminescence Resonance Energy Transfer (NanoBRET) [PDF]
A BRET system is described, in which Nanoluciferase was fused to the lipid transfer protein CERT for efficient energy transfer to a Nile red-labeled ceramide, which is either directly bound to CERT or transported to the adjacent Golgi membrane.
Kirmpaki, Maria-Anna +5 more
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Nano-Luciferase complementation assay of human herpesvirus 8 chemo/cytokine-receptor interactions
Scientific ReportsSome viruses encode proteins that mimic host chemokines and/or cytokines to modulate the host immune responses against infection. Human herpesvirus 8 (HHV-8), also termed Kaposi sarcoma-associated herpesvirus (KSHV), encodes three viral CC-type ...
Vikrant Palande +2 more
doaj +1 more source
STAR ProtocolsSummary: Neoblasts are the only cells capable of proliferation in planarians. The traditional flow cytometry protocol using Hoechst inhibits the cell cycle. Here, we present a protocol for culturing and functionally manipulating planarian neoblasts using
Wenya Zhang +3 more
doaj +1 more source
Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time
Scientific Reports, 2017 Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation.
Carl W. White +4 more
doaj +1 more source
A novel TOX-nanoluciferase reporter mouse for exploring modulators of T cell exhaustion [PDF]
Cytotoxic T cell (CTL) exhaustion is driven by chronic T cell receptor (TCR) stimulation, leading to a dysfunctional state of cells. Exhausted CTLs exhibit diminished effector function against chronic infections and cancers.
den Dekker, Renske J.H. +8 more
core +1 more source
459 Development of small molecules targeting an epigenetic modulator for pediatric neuroblastoma
Journal of Clinical and Translational ScienceObjectives/Goals: The bromodomain PHD finger transcription factor (BPTF) is an oncogenic driver of neuroblastoma. Our objective is to pioneer the discovery of the first class of chemical compounds that engage the PHD finger of BPTF and inhibit its ...
Joseph McPherson +7 more
doaj +1 more source
Self-Assembling Nanoluciferase Fragments as Probes for Alpha-Synuclein Aggregation
, 2018 Alpha-Synuclein (ASYN) is a protein that is abundant in the human brain and is found mainly at the tips of nerve cells (neurons) in specialized structures called presynaptic terminals.
Bilyeu, Camden +2 more
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