Results 131 to 140 of about 63,196 (293)

Combined Long‐Read Genome and Transcriptome Sequencing Establishes Novel Variants in MEGF8 as the Cause for Carpenter Syndrome Type 2

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT Carpenter syndrome type 2 (CRPT2) is a rare autosomal recessive disease mainly characterized by craniosynostosis and polysyndactyly. CRPT2 is the rarer subtype of Carpenter syndrome (CRPTS) and is caused by biallelic variants in the multiple epidermal growth factor‐like domains 8 gene (MEGF8).
Kiana Rashidi, Bhavi P. Modi, Phillip A. Richmond, Massimo Mangino, Loryn Byres, Cassie McDonald, Joshua Dalmann, Simran Samra, Kate L. Del Bel, Anna Lehman, Jessica L. Zambonin, Stuart E. Turvey   +11 more
wiley   +1 more source

One‐step generation of heritable mitochondrial DNA multiplex‐engineered rats using DddA‐derived cytosine base editor

Animal Models and Experimental Medicine, EarlyView.
We established that mixed DdCBE microinjection is an efficient, heritable, and precise strategy for generating multiplex mtDNA mutant rats. This advancement significantly expands the utility of DdCBEs for mitochondrial disease modeling, providing a robust platform for exploring the pathogenic mechanisms of complex mtDNA mutations and developing ...
Xu Zhang, Keru Li, Lei Tan, Wei Chen, Shan Gao, Chenyang Liu, Shuo Pan, Jiayue He, Ning Liu, Gefan Wan, Wei Dong, Weining Kong, Bin Shen, Xiaolong Qi, Yuanwu Ma   +14 more
wiley   +1 more source

Nanopore amplicon sequencing v2

, 2022
This protocol describes the step-by-step procedures for performing nanopore amplicon sequencing using the latest Kit 14 chemistry. The method is versatile and can be applied to a wide range of sequence-based analyses, including human microbiome profiling and biodiversity assessment using environmental DNA (eDNA metabarcoding).
openaire   +1 more source

How does the efficiency of Nanopore compare to Illumina sequencing in tracking the spread of aquatic invasive species?

Water Biology and Security
eDNA-based methods offer a non-invasive, easy-to-use approach for monitoring aquatic biodiversity, particularly for tracking invasive or cryptic species.
Théo Deremarque, Rodolphe Elie Gozlan, Ravo Ravaozafindrasoa, Giuliano Mucci, Lucie Delalex, Jean-Michel Foissy, Michaël Cagnant, Mathieu Clair, Justina Givens, Fabienne Justy, Alice Valentini, Delphine Nicolas, Pascal Contournet, Claire Tetrel, Marc Thibault, Marine Combe   +15 more
doaj   +1 more source

An information theory approach to quantifying the sequence-dependent response of nucleic acid motors with applications to nanopore DNA sequencing

Nature Communications
Motor enzymes that interact with DNA are essential for replicative biological processes. In nanopore sequencing, a motor enzyme controls the motion of a nucleic acid through a protein nanopore, and sequence-dependent blockages of an ion current flowing ...
Jonathan M. Craig, Andrew H. Laszlo, Henry Brinkerhoff, Christopher A. Thomas, Sinduja K. Marx, Eric F. Lebo, Sarah J. Abell, Michaela C. Franzi, Jesse R. Huang, Hwanhee C. Kim, Jessica D. Carrasco, Jens H. Gundlach   +11 more
doaj   +1 more source

Enhanced Rheological and Filtration Performance of Drilling Fluids Using Fe3O4/Saponin/Cu(II) Nanocomposites: Synthesis, Characterization, and Application

Asia-Pacific Journal of Chemical Engineering, EarlyView.
ABSTRACT Drilling fluids used in high‐performance well operations often struggle to maintain rheological stability, colloidal dispersion, and filtration control under harsh downhole conditions. This study engineered a multifunctional Fe3O4@Saponin/Cu(II) nanocomposite to address these challenges.
Kassem Al Attabi, Farag M. A. Altalbawy, Hana Ihsan Hassan, Ahmed Salih Sahib, Zahraa Saad Abdulali, Mariem Alwan, Mahmood Jawad, Hiba Mushtaq, Aseel Smerat, Fatimah Pashtun   +9 more
wiley   +1 more source

More accurate nanopore sequencing [PDF]

Nature Methods, 2019
openaire   +2 more sources

A cost‐effective DNA extraction protocol for long‐read sequencing in non‐model plants

Applications in Plant Sciences, EarlyView.
Abstract Premise Third‐generation sequencing has revolutionized genomics, enabling in‐depth analysis of genome sequence, structure, and epigenetic features. Yet, extracting high‐quality DNA for long‐read sequencing remains a bottleneck—particularly in non‐model plants, such as mature trees growing in natural environments, which often contain abundant ...
Sofia Gaischuk, Cintia F. Marchetti, Ezequiel Petrillo, Fernando A. Rabanal, Detlef Weigel, Nicolás Bellora, M. Verónica Arana   +6 more
wiley   +1 more source

Halide perovskites in wide‐spectrum photodetection

Bulletin of the Korean Chemical Society, EarlyView.
Recent advances in perovskite‐based photodetectors (PPDs), phototransistors, and photoconductors are technically discussed in this review. Moreover, the mechanism behind different harvesting processes in perovskite following the energy of photons is explained, where the gamma or x‐rays are ionizing.
Somayeh Gholipour, Reza Rahighi, Mohammad Panahi, Muhammad Naufal Lintangpradipto, Reza Ghayoor, Michael Saliba   +5 more
wiley   +1 more source

Genetic code expansion for chemosensing and chemical actuation of biological functions

Bulletin of the Korean Chemical Society, EarlyView.
Genetic code expansion (GCE) broadens the chemical repertoire for precise control of protein function in biological systems. This review highlights GCE‐based sensing and actuating systems that operate through selective interactions between noncanonical amino acids and chemical triggers.
Jieun Bae, Dong‐Hyun Kim, Minseob Koh
wiley   +1 more source