Results 51 to 60 of about 38,547 (210)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Evaluation of suitability of substituting wheat flour with sweet potato and tiger nut flours in bread making

Open Agriculture, 2016
The study was carried out to assess the nutritional quality and palatability of bread after partially substituting wheat flour (WF) with sweet potato (SF) and tiger nut (TF) flours.
Ezeocha Chinelo V., Onwuneme Nnenna A.
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Morphological Classification of 52 Accessions of White Yam (Dioscorea rotundata) Species at the National Root Crops Research Institute, Umudike, Abia State, Nigeria

Journal of Applied Sciences and Environmental Management
The classification and identification of white yam (Dioscorea rotundata) species are often confusing and poorly characterized, hindering breeding programmes and conservation efforts. The objective of this paper was classification of 52 Accessions of white yam (Dioscorea rotundata) species at African yam screen house, National Root Crops Research ...
S. I. Akpanudo, J. O. Ekanem, O. V. Andrew, J. S. Nwogha, E. M. Nyahette, B. I. Essien   +5 more
openaire   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Genotype-by-environment interaction and stability of root mealiness and other organoleptic properties of boiled cassava roots

Scientific Reports, 2022
Genetic enhancement of cassava aimed at improving cooking and eating quality traits is a major goal for cassava breeders to address the demand for varieties that are desirable for the fresh consumption market segment.
Kelechi Uchendu, Damian N. Njoku, Ugochukwu N. Ikeogu, Daniel Dzidzienyo, Pangirayi Tongoona, Samuel Offei, Chiedozie Egesi   +6 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Novel SNP markers and other stress-related genomic regions associated with nitrogen use efficiency in cassava

Frontiers in Plant Science
Cassava productivity is constrained by low soil nitrogen, which is predominant in most cassava-growing regions in the tropics and subtropical agroecology. Improving the low nitrogen tolerance of cassava has become an important breeding objective.
Joseph Okpani Mbe, Joseph Okpani Mbe, Daniel Kwadjo Dzidzienyo, Daniel Kwadjo Dzidzienyo, Simon Peter Abah, Simon Peter Abah, Damian Ndubuisi Njoku, Joseph Onyeka, Pangirayi Tongoona, Chiedozie Egesi, Chiedozie Egesi   +10 more
doaj   +1 more source

DESIGN OF SOLAR PHOTOVOLTAIC SYSTEM FOR UNDERGROUND IRRIGATION PUMPING WATER FOR THE AGRICULTURAL FARMLAND IN NATIONAL ROOT CROPS RESEARCH INSTITUTE, UMUDIKE (NRCRI), ABIA STATE, NIGERIA.

Journal of Science and Technology
This study addresses the critical challenge of water scarcity and high energy costs impacting agricultural productivity in regions like Abia State, Nigeria, by proposing a sustainable solar photovoltaic (SPV) irrigation pumping system. The objective was to design and evaluate an SPV system for underground irrigation at the National Root Crops Research ...
Igwe E. Johnson, Hyginus Obinna Ozioko, Igwe C. Harryson, Nwankwojike B. Nduka, Emmanuel Ebube Eze   +4 more
openaire   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source