Results 31 to 40 of about 24,584,878 (168)

Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Anti-GD1a antibodies activate complement and calpain to injure distal motor nodes of Ranvier in mice [PDF]

, 2010
The motor axonal variant of Guillain-Barré syndrome is associated with anti-GD1a immunoglobulin antibodies, which are believed to be the pathogenic factor. In previous studies we have demonstrated the motor terminal to be a vulnerable site.
Halstead, S., Furukawa, K., Humphreys, P.D, Willison, H.J., McGonigal, R., Rowan, E.G., Greenshields, K.N, Rother, R.P   +7 more
core   +2 more sources

Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB₂ in malaria exposed individuals [PDF]

, 2009
Background MB2 protein is a sporozoite surface antigen on the human malaria parasite Plasmodium falciparum. MB2 was identified by screening a P. falciparum sporozoite cDNA expression library using immune sera from a protected donor immunized via the ...
James Anthony A, de la Vega, Patricia, John, Chandy C, Thanh V Nguyen, Nguyen, Thanh V, de la Vega, P, John B Sacci, Patricia de la Vega, James, Anthony A, Kang Angray S, John Chandy C, James, AA, Nguyen, T.V., Sacci, John B, Kang, A.S., Sacci John B, de la Vega, P., Nguyen Thanh V, Anthony A James, de la Vega Patricia, John, C.C., Kang, Angray S, Angray S Kang, Sacci, J.B., Patricia Vega, Chandy C John, John, CC, Nguyen, TV, Kang, AS, Jr, SJB, James, A.A.   +30 more
core   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis [PDF]

, 2009
Antibodies to citrulline-modifi ed proteins have a high diagnostic value in rheumatoid arthritis (RA). However, their biological role in disease development is still unclear.
Rikard Holmdahl, Bockermann, Robert, Hüseyin Uysal, Sehnert, Bettina,, Harald Burkhardt, Robert Bockermann, Bockermann, R, Nandakumar, Kutty S, Bajtner, Estelle,, Engstrom, A, Thunnissen, Marjolein M G M, Uysal, H,, Serre, Guy, Serre, G, Holmdahl, R,, Nandakumar, Kutty Selva,, Bajtner, E, Holmdahl, Rikard, Uysal, Hüseyin,, Burkhardt, Harald,, Burkhardt, H, Kutty Selva, Nandakumar,, Kutty S. Nandakumar, Bettina Sehnert, Thunnissen, MMGM, Serre, Guy,, Nandakumar, Kutty S., Burkhardt, Harald, Engström, Åke, Thunnissen, Marjolein,, Guy Serre, Engström, Åke,, Thunnissen, Marjolein M. G. M., Thunnissen, Marjolein M. G. M.,, Sehnert, B, Åke Engström, Sehnert, Bettina, Kutty Selva, Nandakumar, Nandakumar, KS, Estelle Bajtner, Lund University., Bajtner, Estelle, Thunnissen, Marjolein, Marjolein M.G.M. Thunnissen, Bockermann, Robert,, Holmdahl, Rikard,, Uysal, Hüseyin   +46 more
core   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source

An investigation of the molecular basis of interactions between human monoclonal antibodies and antigens that are clinically relevant in systemic lupus erythematosus and the Antiphospholipid Syndrome. [PDF]

, 2007
Autontibodies to a wide variety of antigens are associated with systemic lupus erythematosus (SLE) and the Antiphospholipid Syndrome (APS).
Lambrianides, A.
core