Results 111 to 120 of about 436,148 (290)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Estimating bolt tightness using transverse natural frequencies [PDF]
, 2018 Structural health monitoring techniques based on vibration measurements have been receiving large attention in the last decades, including techniques for estimating bolted joint tightness and detecting loosened bolts. Due to the exposure of bolted joints
Fidlin, Alexander +14 more
core +1 more source
Experimental Definition of Defects in the Material of the Elastic Body
Механика машин, механизмов и материалов, 2012 In given article it is offered to use own forms of fluctuations as a diagnostic sign for detection of defects in a material of an elastic body. At realization of a way choose some own forms, define supervision parameters in various points of reference ...
A.V. Kasitsyn +2 more
doaj
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Using neural networks to predict the effect of the preload location on the natural frequencies of a cantilever beam. [PDF]
Heliyon, 2022 Paridie AM, Ene NM, Mohamed YS.
europepmc +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Natural frequencies of neural activities and cognitions may serve as precise targets of rhythmic interventions to the aging brain. [PDF]
Front Aging Neurosci, 2022 Qiao J, Wang Y, Wang S.
europepmc +1 more source
FEBS Open Bio, EarlyView.Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley +1 more source
FACILITATING EFFECT OF NATURAL FREQUENCIES: SIZE DOES NOT MATTER [PDF]
, 2009 The question of whether humans are able to work in a Bayesian way is currently a topic of substantial investigation. An important finding, reported by Gigerenzer and Hoffrage in 1995 is that Bayesian reasoning is facilitated when the information format ...
R. Misuraca +7 more
core +1 more source