Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Identification of the Natural Frequencies of Oscillations of Perforated Vibrosurfaces with Holes of Complex Geometry. [PDF]
Materials (Basel), 2023 Kharchenko S +5 more
europepmc +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Control of deposits on pipeline systems by the method of free oscillations
Известия высших учебных заведений: Проблемы энергетикиRELEVANCE. There are a large number of heat exchangers in operation enterprises that operate under various temperature conditions. Steam, hot water, heated products of oil refining and other industries, which may contain oxides of iron, aluminum, calcium
E. V. Garnyshova, E. V. Izmaylova
doaj +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis
FEBS Open Bio, EarlyView.MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss +7 more
wiley +1 more source
Influence of the nonlocal parameter on the transverse vibration of double-walled carbon nanotubes
Journal of the Mechanical Behavior of Materials, 2015 A high-order nonlocal continuum beam model is proposed, which can be applied to study the transverse vibrations of double-walled carbon nanotubes (DWCNTs), including those that could have initial deformations due to defects or external actions.
de Borbón Fernanda, Ambrosini Daniel
doaj +1 more source
Using neural networks to predict the effect of the preload location on the natural frequencies of a cantilever beam. [PDF]
Heliyon, 2022 Paridie AM, Ene NM, Mohamed YS.
europepmc +1 more source
On the bipenalty method: why is it advantageous to add stiffness and mass [PDF]
, 2011 In a recent paper, Askes et al [1] proposed the simultaneous use of stiffness and inertia of large magnitude to model constraints in time domain analysis.
Ilanko, Sinniah +1 more
core
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
FEBS Open Bio, EarlyView.HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source