Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer [PDF]
Frontiers in Microbiology, 2021 Riemerella anatipestifer is a gram-negative bacterium that is the first naturally competent bacterium identified in the family Flavobacteriaceae. However, the determinants that influence the natural transformation and the underlying mechanism remain ...
Li Zhang +55 more
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The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation. [PDF]
Mol Microbiol, 2021 In the process of natural transformation bacteria import extracellular DNA molecules for integration into their genome. One strand of the incoming DNA molecule is degraded, whereas the remaining strand is transported across the cytoplasmic membrane.
Silale A, Lea SM, Berks BC.
europepmc +2 more sources
Genetic modification of Streptococcus dysgalactiae by natural transformation [PDF]
mSphereStreptococcus dysgalactiae is an emerging human and animal pathogen. Functional studies of genes involved in virulence of S. dysgalactiae and other pyogenic group streptococci are often hampered by limited genetic tractability.
Marita Torrissen Mårli +4 more
doaj +2 more sources
Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation
mSphere, 2020 Natural transformation is a broadly conserved mechanism of horizontal gene transfer (HGT) in bacteria that can shape their evolution through the acquisition of genes that promote virulence, antibiotic resistance, and other traits.
Roberto C. Molina-Quiroz +4 more
doaj +2 more sources
Automating Cloning by Natural Transformation. [PDF]
ACS Synthetic Biology, 2020 Affordable and automated cloning platforms are essential to many synthetic biology studies. However, the traditional E. coli-based cloning is a major bottleneck as it requires heat shock or electroporation implemented in the robotic workflows.
Xinglin Jiang +7 more
semanticscholar +5 more sources
Markerless gene deletion in Ralstonia solanacearum based on its natural transformation competence [PDF]
Frontiers in Microbiology, 2022 Ralstonia solanacearum species complex (RSSC) is a group of Gram-negative bacterial pathogen capable of infecting numerous plants and crops, causing severe vascular wilt diseases.
Jinli Yan +15 more
doaj +2 more sources
The molecular diversity of transcriptional factor TfoX is a determinant in natural transformation in Glaesserella parasuis [PDF]
Frontiers in Microbiology, 2022 Natural transformation is a mechanism by which a particular bacterial species takes up foreign DNA and integrates it into its genome. The swine pathogen Glaesserella parasuis (G. parasuis) is a naturally transformable bacterium.
Xiaoyu Tang +19 more
doaj +2 more sources
An Improved Natural Transformation Protocol for the Cyanobacterium Synechocystis sp. PCC 6803
Frontiers in Plant Science, 2020 The naturally transformable cyanobacterium Synechocystis sp. PCC 6803 is a widely used chassis strain for the photosynthetic production of chemicals. However, Synechocystis possesses multiple genome copies per cell which means that segregating mutations ...
Matthew A. Pope +2 more
doaj +2 more sources
Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis [PDF]
Frontiers in Microbiology, 2021 In our previous study, it was shown that Riemerella anatipestifer, a Gram-negative bacterium, is naturally competent, but the genes involved in the process of natural transformation remain largely unknown.
Li Huang +51 more
doaj +2 more sources
Involvement of the Histone-Like Nucleoid Structuring Protein (H-NS) in Acinetobacter baumannii’s Natural Transformation [PDF]
Pathogens, 2021 Most Acinetobacter baumannii strains are naturally competent. Although some information is available about factors that enhance or reduce the frequency of the transformation of this bacterium, the regulatory elements and mechanisms are barely understood.
Casin Le +11 more
doaj +2 more sources