Results 1 to 10 of about 871 (124)

High-throughput generic single-entity sequencing using droplet microfluidics. [PDF]

open access: yesImeta
We present Generic Single Entity Sequencing (GSE‐Seq), a droplet‐based workflow that generates monoclonal barcodes by one‐step PCR, performs dissolvable hydrogel‐enabled reactions for genome processing, and attaches barcodes during in‐droplet library preparation. Barcoded fragments are pooled and sequenced with PacBio long‐read sequencing. Barcodes are
Wang G   +14 more
europepmc   +2 more sources

Giant viruses as reservoirs of antibiotic resistance genes [PDF]

open access: yesNature Communications
Nucleocytoplasmic large DNA viruses (NCLDVs; also called giant viruses), constituting the phylum Nucleocytoviricota, can infect a wide range of eukaryotes and exchange genetic material with not only their hosts but also prokaryotes and phages.
Xinzhu Yi   +13 more
doaj   +2 more sources

Covariance of Marine Nucleocytoplasmic Large DNA Viruses with Eukaryotic Plankton Communities in the Sub-Arctic Kongsfjorden Ecosystem: A Metagenomic Analysis of Marine Microbial Ecosystems [PDF]

open access: yesMicroorganisms, 2023
Nucleocytoplasmic large DNA viruses (NCLDVs) infect various marine eukaryotes. However, little is known about NCLDV diversity and their relationships with eukaryotic hosts in marine environments, the elucidation of which will advance the current ...
Kang Eun Kim   +7 more
doaj   +2 more sources

Quantitative Assessment of Nucleocytoplasmic Large DNA Virus and Host Interactions Predicted by Co-occurrence Analyses

open access: yesMSphere, 2021
NCLDVs can infect a wide range of eukaryotes, although their life cycle is less dependent on hosts compared to other viruses. However, our understanding of NCLDV-host systems is highly limited because few of these viruses have been isolated so far.
Lingjie Meng   +2 more
exaly   +3 more sources

Advantages and Limits of Metagenomic Assembly and Binning of a Giant Virus

open access: yesMSystems, 2020
Giant viruses have large genomes, often within the size range of cellular organisms. This distinguishes them from most other viruses and demands additional effort for the successful recovery of their genomes from environmental sequence data.
Frederik Schulz   +2 more
exaly   +5 more sources

Hidden evolutionary complexity of Nucleo-Cytoplasmic Large DNA viruses of eukaryotes

open access: yesVirology Journal, 2012
Background The Nucleo-Cytoplasmic Large DNA Viruses (NCLDV) constitute an apparently monophyletic group that consists of at least 6 families of viruses infecting a broad variety of eukaryotic hosts.
Natalya Yutin   +2 more
exaly   +3 more sources

Promoter Motifs in NCLDVs: An Evolutionary Perspective

open access: yesViruses, 2017
For many years, gene expression in the three cellular domains has been studied in an attempt to discover sequences associated with the regulation of the transcription process. Some specific transcriptional features were described in viruses, although few
GRAZIELE Pereira Oliveira   +2 more
exaly   +3 more sources

Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution

open access: yesVirology Journal, 2009
Background The Nucleo-Cytoplasmic Large DNA Viruses (NCLDV) comprise an apparently monophyletic class of viruses that infect a broad variety of eukaryotic hosts.
Natalya Yutin   +2 more
exaly   +3 more sources

Evolution of DNA ligases of Nucleo-Cytoplasmic Large DNA viruses of eukaryotes: a case of hidden complexity

open access: yesBiology Direct, 2009
Background Eukaryotic Nucleo-Cytoplasmic Large DNA Viruses (NCLDV) encode most if not all of the enzymes involved in their DNA replication. It has been inferred that genes for these enzymes were already present in the last common ancestor of the NCLDV ...
Natalya Yutin   +2 more
exaly   +3 more sources

Genomic and biogeographic characterisation of the novel prasinovirus Mantoniella tinhauana virus 1. [PDF]

open access: yesEnviron Microbiol Rep
Phylogenetic tree, virion morphology and global metagenomic abundance of Mantoniella tinhauana virus 1, a newly isolated and characterised prasinovirus. Abstract Mamiellophyceae are a ubiquitous class of unicellular green algae in the global ocean.
Rey Redondo E, Leung SKK, Yung CCM.
europepmc   +2 more sources

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