Isolation and Characterization of a Double Stranded DNA Megavirus Infecting the Toxin-Producing Haptophyte Prymnesium parvum [PDF]
Prymnesium parvum is a toxin-producing haptophyte that causes harmful algal blooms globally, leading to large-scale fish kills that have severe ecological and economic implications.
Ben A. Wagstaff +5 more
doaj +5 more sources
Dynamic genome evolution and complex virocell metabolism of globally-distributed giant viruses
Nucleocytoplasmic large DNA viruses (NCLDV) exhibit a large genomic repertoire and complex evolutionary history. Here, the authors generate 501 metagenome-assembled genomes from diverse environments and show NCLDVs to harbor a wide range of potential ...
Mohammad Moniruzzaman +2 more
exaly +2 more sources
Phaeocystis globosa Virus DNA Polymerase X: a “Swiss Army knife”, Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair [PDF]
Phaeocystis globosa virus 16T is a giant virus that belongs to the so-called nucleo-cytoplasmic large DNA virus (NCLDV) group. Its linear dsDNA genome contains an almost full complement of genes required to participate in viral base excision repair (BER).
José L. Fernández-García +3 more
doaj +5 more sources
Background Nucleo-Cytoplasmic Large DNA viruses (NCLDV), a diverse group that infects a wide range of eukaryotic hosts, exhibit a large heterogeneity in genome size (between 100 kb and 1.2 Mb) but have been suggested to form a monophyletic group on the ...
Jonathan Filée
exaly +3 more sources
The nucleocytoplasmic large DNA viruses (NCLDV) of eukaryotes (proposed order, “Megavirales”) include the families Poxviridae, Asfarviridae, Iridoviridae, Ascoviridae, Phycodnaviridae, Marseilleviridae, and Mimiviridae, as well as still unclassified ...
Natalya Yutin +2 more
exaly +3 more sources
Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus [PDF]
African swine fever virus (ASFV) belongs to the family of Asfarviridae, part of the group of nucleocytoplasmic large DNA viruses (NCLDV). Little is known about the internalization of ASFV in the host cell and the fusion membrane events that take place at
Jesús Urquiza +6 more
doaj +2 more sources
Characterization of Medusavirus encoded histones reveals nucleosome-like structures and a unique linker histone [PDF]
The organization of DNA into nucleosomes is a ubiquitous and ancestral feature that was once thought to be exclusive to the eukaryotic domain of life. Intriguingly, several representatives of the Nucleocytoplasmic Large DNA Viruses (NCLDV) encode histone-
Chelsea M. Toner +3 more
doaj +2 more sources
High-throughput generic single-entity sequencing using droplet microfluidics. [PDF]
We present Generic Single Entity Sequencing (GSE‐Seq), a droplet‐based workflow that generates monoclonal barcodes by one‐step PCR, performs dissolvable hydrogel‐enabled reactions for genome processing, and attaches barcodes during in‐droplet library preparation. Barcoded fragments are pooled and sequenced with PacBio long‐read sequencing. Barcodes are
Wang G +14 more
europepmc +2 more sources
ViralRecall—A Flexible Command-Line Tool for the Detection of Giant Virus Signatures in ‘Omic Data
Giant viruses are widespread in the biosphere and play important roles in biogeochemical cycling and host genome evolution. Also known as nucleo-cytoplasmic large DNA viruses (NCLDVs), these eukaryotic viruses harbor the largest and most complex viral ...
Frank O. Aylward, Mohammad Moniruzzaman
doaj +1 more source
The nucleocytoplasmic large DNA viruses (NCLDV) possess unique characteristics that have drawn the attention of the scientific community, and they are now classified in the phylum Nucleocytoviricota.
Fernanda Gil de Souza +2 more
doaj +1 more source

