Results 11 to 20 of about 847 (121)

High-throughput generic single-entity sequencing using droplet microfluidics. [PDF]

Imeta
We present Generic Single Entity Sequencing (GSE‐Seq), a droplet‐based workflow that generates monoclonal barcodes by one‐step PCR, performs dissolvable hydrogel‐enabled reactions for genome processing, and attaches barcodes during in‐droplet library preparation. Barcoded fragments are pooled and sequenced with PacBio long‐read sequencing. Barcodes are
Wang G, Zhao L, Shi Y, Qu F, Ding Y, Liu W, Liu C, Luo G, Li M, Bai X, Li L, Wang L, Wong CC, Ho YP, Yu J.   +14 more
europepmc   +2 more sources

Genomic and biogeographic characterisation of the novel prasinovirus Mantoniella tinhauana virus 1. [PDF]

Environ Microbiol Rep
Phylogenetic tree, virion morphology and global metagenomic abundance of Mantoniella tinhauana virus 1, a newly isolated and characterised prasinovirus. Abstract Mamiellophyceae are a ubiquitous class of unicellular green algae in the global ocean.
Rey Redondo E, Leung SKK, Yung CCM.
europepmc   +2 more sources

Quantitative Assessment of Nucleocytoplasmic Large DNA Virus and Host Interactions Predicted by Co-occurrence Analyses

mSphere, 2021
NCLDVs can infect a wide range of eukaryotes, although their life cycle is less dependent on hosts compared to other viruses. However, our understanding of NCLDV-host systems is highly limited because few of these viruses have been isolated so far.
Lingjie Meng, Hisashi Endo, Romain Blanc-Mathieu, Samuel Chaffron, Rodrigo Hernández-Velázquez, Hiroto Kaneko, Hiroyuki Ogata   +6 more
doaj   +1 more source

Metatranscriptomics reveals a shift in microbial community composition and function during summer months in a coastal marine environment

Environmental DNA, Volume 5, Issue 5, Page 920-933, September 2023., 2023
The composition and microbial activity of marine water is expected to vary with seasons and abiotic variables. To characterize both the microbial compositional and functional changes associated with abiotic factors such as pH, temperature, alkalinity, aragonite, calcite and pCO2, here we generate a reference metatranscriptome from the Strait of Georgia,
Ben J. G. Sutherland, Jan F. Finke, Robert Saunders, Snehal Warne, Angela D. Schulze, Jeff H. T. Strohm, Amy M. Chan, Curtis A. Suttle, Kristina M. Miller   +8 more
wiley   +1 more source

Tracing the Origin of Genotype II African Swine Fever Virus in China by Genomic Epidemiology Analysis

Transboundary and Emerging Diseases, Volume 2023, Issue 1, 2023., 2023
The pandemic spread of African swine fever (ASF) has caused serious effects on the global pig industry. Virus genome sequencing and genomic epidemiology analysis play an important role in tracking the outbreaks of the disease and tracing the transmission of the virus.
Yong Zhang, Qinghua Wang, Zhongyi Zhu, Shujuan Wang, Shuyang Tu, Yongqiang Zhang, Yanli Zou, Yutian Liu, Chunju Liu, Weijie Ren, Dongxia Zheng, Yunling Zhao, Yongxin Hu, Lin Li, Chuan Shi, Shengqiang Ge, Peng Lin, Fengping Xu, Jinmin Ma, Xiaodong Wu, Hongchao Ma, Zhiliang Wang, Jingyue Bao, Jordi Casal   +23 more
wiley   +1 more source

Informational gene phylogenies do not support a fourth domain of life for nucleocytoplasmic large DNA viruses. [PDF]

PLoS ONE, 2011
Mimivirus is a nucleocytoplasmic large DNA virus (NCLDV) with a genome size (1.2 Mb) and coding capacity ( 1000 genes) comparable to that of some cellular organisms.
Tom A Williams, T Martin Embley, Eva Heinz   +2 more
doaj   +1 more source

Bacteriophages pass through candle‐shaped porous ceramic filters: Application for the collection of viruses in soil water

MicrobiologyOpen, Volume 11, Issue 5, October 2022., 2022
This study proposes to use porous candles as a new tool for collecting soil water viruses. In the first step, the passage of model viruses through the ceramic part of the candles was investigated. Regarding the field application, the experiments carried out showed a successful recovery of viruses, demonstrating the relevance and efficiency of such a ...
Perrine Florent, Henry‐Michel Cauchie, Malte Herold, Leslie Ogorzaly   +3 more
wiley   +1 more source

A comparative study of flow cytometry‐sorted communities and shotgun viral metagenomics in a Singapore municipal wastewater treatment plant

iMeta, Volume 1, Issue 3, September 2022., 2022
This study elucidates the practical protocol of applying fluorescence‐activated cell sorting (FACS) and metagenomics on DNA and RNA viromes. This is the first systematic analysis of viral metagenomics in a municipal wastewater treatment plant with the Modified Ludzack–Ettinger (MLE) process.
Xiaoqiong Gu, Yi Yang, Feijian Mao, Wei Lin Lee, Federica Armas, Fang You, David M. Needham, Charmaine Ng, Hongjie Chen, Franciscus Chandra, Karina Yew‐Hoong Gin   +10 more
wiley   +1 more source

Hidden evolutionary complexity of Nucleo-Cytoplasmic Large DNA viruses of eukaryotes

Virology Journal, 2012
Background The Nucleo-Cytoplasmic Large DNA Viruses (NCLDV) constitute an apparently monophyletic group that consists of at least 6 families of viruses infecting a broad variety of eukaryotic hosts.
Yutin Natalya, Koonin Eugene V
doaj   +1 more source

Adaptive evolution of viruses infecting marine microalgae (haptophytes), from acute infections to stable coexistence

Biological Reviews, Volume 97, Issue 1, Page 179-194, February 2022., 2022
ABSTRACT Collectively known as phytoplankton, photosynthetic microbes form the base of the marine food web, and account for up to half of the primary production on Earth. Haptophytes are key components of this phytoplankton community, playing important roles both as primary producers and as mixotrophs that graze on bacteria and protists.
Ruth‐Anne Sandaa, Marius R. Saltvedt, Håkon Dahle, Haina Wang, Selina Våge, Romain Blanc‐Mathieu, Ida H. Steen, Nigel Grimsley, Bente Edvardsen, Hiroyuki Ogata, Janice Lawrence   +10 more
wiley   +1 more source