Crop Journal, 2020 Rye (Secale cereale L.) has been widely used to improve wheat (Triticum aestivum L.) cultivars. Oligo probes combined with non-denaturing fluorescence in situ hybridization (ND-FISH) technology provide a convenient and efficient way to identify ...
Wei Xi +5 more
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Novel simple sequence repeats (SSRs) detected by ND-FISH in heterochromatin of
BMC Genomics, 2011 Background In recent years, substantial progress has been made in understanding the organization of sequences in heterochromatin regions containing single-copy genes and transposable elements.
Jouve Nicolás, Cuadrado Ángeles
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Development and application of universal ND-FISH probes for detecting P-genome chromosomes based on Agropyron cristatum transposable elements. [PDF]
Mol Breed, 2022 Fluorescence in situ hybridization (FISH) is a basic tool that is widely used in cytogenetic research. The detection efficiency of conventional FISH is limited due to its time-consuming nature. Oligonucleotide (oligo) probes with fluorescent labels have been applied in non-denaturing FISH (ND-FISH) assays, which greatly streamline experimental ...
Sun Y +13 more
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New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds. [PDF]
Int J Mol Sci, 2019 Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds.
Xi W +5 more
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Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes. [PDF]
Sci Rep, 2015 AbstractGenomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of ...
Fu S +8 more
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New Oligonucleotide Probes for ND-FISH Analysis to Identify Barley Chromosomes and to Investigate Polymorphisms of Wheat Chromosomes. [PDF]
Genes (Basel), 2016 Oligonucleotide probes that can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) analysis are convenient tools for identifying chromosomes of wheat (Triticum aestivum L.) and its relatives. New oligonucleotide probes, Oligo-HvT01, Oligo-pTa71-1, Oligo-s120.1, Oligo-s120.2, Oligo-s120.3, Oligo-275.1, Oligo-275.2, Oligo-k566 and ...
Tang S, Qiu L, Xiao Z, Fu S, Tang Z.
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Frequent variations and phylogenetic relationships within the genus Secale identified by ND-FISH according to the genome-wide universal oligonucleotides chromosome probes. [PDF]
Front Plant SciIntroductionRye (Secale cereale L.) played a very important role in wheat genetic improvement and forage production worldwide. However, since rye is a kind of cross-pollinated plant, high levels of genetic heterozygosity and heterogeneity existed in the genome.
Li Z, Sun Z, Ren T.
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Evaluating the effect of γ-oryzanol on MASLD pathology using a medaka fish model. [PDF]
FEBS Open BioThis study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Ito Y +7 more
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Global Ecology and Conservation, 2023 Grasslands are among the most impacted ecosystems globally. In the midcontinent of North America, a > 80% loss of grasslands has made their conservation a major priority for resource managers.
Michael J. Anteau +5 more
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Experimental Investigation of a Prototype Thermal Refuge for Trout
Energies, 2022 Recent years have seen an increase in surface water temperatures in several streams and fisheries, which has a detrimental effect on cold-water species such as trout.
Rajib Uddin Rony +2 more
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