Results 1 to 10 of about 47,286 (121)

The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH [PDF]

Molecules, 2019
Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome.
Tianheng Ren, Maojie He, Zixin Sun, Feiquan Tan, Peigao Luo, Zongxiang Tang, Shulan Fu, Benju Yan, Zhenglong Ren, Zhi Li   +9 more
doaj   +5 more sources

Chromosome Rearrangement in Elymus dahuricus Revealed by ND-FISH and Oligo-FISH Painting [PDF]

Plants, 2023
As a perennial herb in Triticeae, Elymus dahuricus is widely distributed in Qinghai–Tibetan Plateau and Central Asia. It has been used as high-quality fodders for improving degraded grassland. The genomic constitution of E.
Chengzhi Jiang, Xiaodan Liu, Zujun Yang, Guangrong Li   +3 more
doaj   +4 more sources

Precise Identification of Chromosome Constitution and Rearrangements in Wheat–Thinopyrum intermedium Derivatives by ND-FISH and Oligo-FISH Painting [PDF]

Plants, 2022
Thinopyrum intermedium possesses many desirable agronomic traits that make it a valuable genetic source for wheat improvement. The precise identification of individual chromosomes of allohexaploid Th.
Zhihui Yu, Hongjin Wang, Ennian Yang, Guangrong Li, Zujun Yang   +4 more
doaj   +4 more sources

Chromosomal analysis of progenies between Lilium intersectional hybrids and wild species using ND-FISH and GISH [PDF]

Frontiers in Plant Science
IntroductionIntersectional hybrids in lilies possess significant breeding value, but the lack of complete lily genomes and complex genotypes pose challenges for early identification of lily hybrids. This study aimed to use intersectional hybrid cultivars
Mei Zhou, Xue Yong, Jungang Zhu, Qian Xu, Xiaodan Liu, Lu Zhang, Lisha Mou, Lijia Zeng, Mengxi Wu, Beibei Jiang, Yin Jia, Peihua Zhang, Yuanzhi Pan   +12 more
doaj   +4 more sources

ND-FISH with New Oligo Probes for Chromosome Identification of Cichorium intybus Revealing Karyotypic Variation and Divergence of Asteraceae Species [PDF]

Plants
Chicory (Cichorium intybus L., 2n = 18), belonging to the Asteraceae family, exhibits significant edible, medicinal, and pasture values. Moderate research has been performed on identifying Chicory species’ chromosomes using fluorescence in situ ...
Meiling Chen, Chengzhi Jiang, Doudou Huang, Zhiqiang Zheng, Wenzhuo Yang, Guangrong Li, Chun Fu, Hong Liao, Wencong Long, Zujun Yang, Yaojun Yang   +10 more
doaj   +4 more sources

Developing New Oligo Probes to Distinguish Specific Chromosomal Segments and the A, B, D Genomes of Wheat (Triticum aestivum L.) Using ND-FISH [PDF]

Frontiers in Plant Science, 2018
Non-denaturing FISH (ND-FISH) technology has been widely used to study the chromosomes of Triticeae species because of its convenience. The oligo probes for ND-FISH analysis of wheat (Triticum aestivum L.) chromosomes are still limited.
Shuyao Tang, Shuyao Tang, Zongxiang Tang, Zongxiang Tang, Ling Qiu, Zujun Yang, Guangrong Li, Tao Lang, Wenqian Zhu, Wenqian Zhu, Jiehong Zhang, Shulan Fu   +11 more
doaj   +5 more sources

Oligonucleotides and ND-FISH Displaying Different Arrangements of Tandem Repeats and Identification of Dasypyrum villosum Chromosomes in Wheat Backgrounds [PDF]

Molecules, 2017
Oligonucleotide probes and the non-denaturing fluorescence in situ hybridization (ND-FISH) technique are widely used to analyze plant chromosomes because they are convenient tools. New oligonucleotide probes, Oligo-Ku, Oligo-3B117.1, Oligo-3B117.2, Oligo-
Zhiqiang Xiao, Shuyao Tang, Ling Qiu, Zongxiang Tang, Shulan Fu   +4 more
doaj   +5 more sources

Development and application of universal ND-FISH probes for detecting P-genome chromosomes based on Agropyron cristatum transposable elements. [PDF]

Mol Breed, 2022
Fluorescence in situ hybridization (FISH) is a basic tool that is widely used in cytogenetic research. The detection efficiency of conventional FISH is limited due to its time-consuming nature. Oligonucleotide (oligo) probes with fluorescent labels have been applied in non-denaturing FISH (ND-FISH) assays, which greatly streamline experimental ...
Sun Y, Han H, Wang X, Han B, Zhou S, Zhang M, Liu W, Li X, Guo X, Lu Y, Yang X, Zhang J, Liu X, Li L.   +13 more
europepmc   +3 more sources

New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds. [PDF]

Int J Mol Sci, 2019
Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds.
Xi W, Tang Z, Tang S, Yang Z, Luo J, Fu S.   +5 more
europepmc   +4 more sources

Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes. [PDF]

Sci Rep, 2015
AbstractGenomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of ...
Fu S, Chen L, Wang Y, Li M, Yang Z, Qiu L, Yan B, Ren Z, Tang Z.   +8 more
europepmc   +4 more sources